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Xspecies project Wuhan 2011. Xspecies project R / BioConductor training AffylmGUI training Xspecies analysis training Normalisation, filtering and annotation.CDF,

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Presentation on theme: "Xspecies project Wuhan 2011. Xspecies project R / BioConductor training AffylmGUI training Xspecies analysis training Normalisation, filtering and annotation.CDF,"— Presentation transcript:

1 Xspecies project Wuhan 2011

2 Xspecies project

3 R / BioConductor training AffylmGUI training Xspecies analysis training Normalisation, filtering and annotation.CDF, filtering, stats and annotation RMA Normalisation

4 Xspecies project Hevea Brasiliensis Rubber Tree Chin Wei (Jonathan) Ong Samples from Kew Gardens

5 Xspecies project PxLyS1 PxLyS2 PxLyS3 RNAlater Sampling strategy Two plants (young / old) Three samples per leaf complex 6 total chips. Annotation: PxLySz Sampling strategy Two plants (young / old) Three samples per leaf complex 6 total chips. Annotation: PxLySz cRNA gDNA.cel Xspecies CDF100 RNA.cel

6 Xspecies project Chinese Purpleblow Maple (Acer truncatum) Yuan Bao Feng Zhongxiu Zhang Xspecies CDF150 Horqin Desert Pilot Sampling strategy 7 plants (6 month old) - 7 total chips. 3 Beijing Cultivar 3 Horquin Wildtype 1 drought challenged from Beijing Cultivar Pilot Sampling strategy 7 plants (6 month old) - 7 total chips. 3 Beijing Cultivar 3 Horquin Wildtype 1 drought challenged from Beijing Cultivar

7 Xspecies project Saccharum sp. Sugar Cane Natalia Martins Xspecies CDF150 Sampling strategy 3 stages x 3 replicates = 9 total chips Sampling strategy 3 stages x 3 replicates = 9 total chips T1 T2 T3

8 Xspecies project Mucuna pruriens var. utilis Velvetbean Sandeepkumar Patil Xspecies CDF100 Sampling strategy Many plants (3 month old) 3 root samples 3 shot samples = 6 total chips Sampling strategy Many plants (3 month old) 3 root samples 3 shot samples = 6 total chips

9 Xspecies project Remember to do the analysis - and + The cross species CDF 400 1000


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