1. Membranes: Structure & Function Biology 11 E. McIntyre

2. Properties of the plasma membrane Cells live in a fluid environment (i.e. blood, water, extra- cellular fluid). Cells exchange materials with this fluid. Each cell is a separate unit of life (remember cell theory?) It therefore has a boundary – the plasma membrane (AKA cell membrane).

3. ...Properties of the plasma membrane The plasma membrane is the cell’s “gate keeper” It is selectively permeable. It allows the passage of some materials in and out of the cell but not others. Ex a tea bag allows passage of H 2 O but not the leaves. The plasma membrane brings in the ‘good’ things and keeps them in, and it exports the ‘bad’ things and keeps them out.

4. ...Properties of the plasma membrane Small particles pass through easily. Lipids (and particles that are soluble in lipids) pass through with least difficulty. The plasma membrane tends not to be permeable to polar molecules unless they are small. lipid: –an organic compound with Carbon, Hydrogen and Oxygen. –The # of hydrogen atoms are MUCH greater than the # of oxygen atoms. –ex: fats, oils, waxes.

5. magnification of ~200 000X reveals the plasma membrane as having two layers. each of these layers are made of phospholipids, hence the term phospholipid bilayer. Structure of the plasma membrane

6. ...Structure of the plasma membrane a phospholipid molecule is made of two fatty acid tails, glycerol and a phosphate group.

8. Think Critically:Suppose you added some phospholipids to water. If you could see the individual molecules, how do you suppose they would be arranged? Draw a sketch. phosphate groups are polar and are therefore hydrophilic. They will point to the watery environments inside and outside the cell. the fatty acid tails are hydrophobic. They avoid water and seek ‘each other’ The properties of fatty acid molecules are the reason for the plasma membrane’s structure. It forms spontaneously. No energy is required of the cell to maintain the integrity of the plasma membrane. The plasma membrane is often referred to as a “phospholipid bilayer”...Structure of the plasma membrane

10. Why do the phospholipids assume this orientation?

11. ...Structure of the plasma membrane polar molecules have charged ends (+ and -) and are therefore soluble in water (another polar molecule) They are hydrophilic. non- polar molecules are insoluble in water. They are hydrophobic (avoid water).

12. The plasma membrane is fluid-like. The phospholipids can move and slide past each other. (pool-full-of-balls analogy). In some cells, cholesterol molecules bind to the phospholipids to help restrict their movement....Structure of the plasma membrane

13. Membrane Proteins the plasma membrane is embedded with proteins. They have polar and non-polar ends to determine their position in the bilayer. They can move sideways throughout the plasma membrane. The roles of these proteins include: regulation of the movement of particles across membrane; involvement in chemical reactions; act as ‘marker’ on the cell. Biologists now use the fluid mosaic model to describe the plasma membrane. Why is this term appropriate?

14. THINK! Draw two sketches of a plasma membranes with embedded membrane proteins. Label the parts of the proteins that are hydrophillic & hydrophobic –Drawing # 1 : protein goes through entire membrane –Drawing # 2 : protein does not pass through to inside of membrane

17. Osmosis Osmosis: a fancy word for diffusion of water across a semi­-permeable membrane. –occurs from a region of higher concentration of water to a region of low concentration of water. –Water molecules are small and can diffuse through cell membranes. The direction of the diffusion depends only on the difference in concentration of water on each side of the membrane. EFFECTS OF OSMOSIS Movement of water into or out of the cell depends on the concentration gradient. Cells will react differently to different concentrations of water. Isotonic solution: this solution has the same concentration of dissolved substances in the water as a living cell placed in it. Therefore there is no concentration gradient and the cell does not have a gain, or loss of water. Hypotonic solution: this solution contains a lower concentration of dissolved substances than that of a living cell. Therefore the concentration of water molecules outside the cell is higher than that inside the cell, creating a concentration gradient. This results in a movement of water into the cell (osmosis). animal cell placed in hypotonic solution  swells and bursts due to osmotic pressure. A plant cell placed a hypotonic solution  the large vacuole (which stores water) swells to fill the interior of the cell, pushing the cell contents against the cell wall. The resulting pressure in the plant cell is known as turgor pressure. Hypertonic solution: this solution contains a higher concentration of dissolved substances than that of the living cell. Therefore the concentration of water molecules inside the cell is higher than that outside the cell, creating a concentration gradient. This results in a movement of water out of the cell. An animal cell placed in a hypertonic solution will shrink. In a plant cell placed in a hypertonic solution, the vacuole collapses, causing the cytoplasm to shrink. This shrinking of cytoplasm by osmosis is called plasmolysis.

18. In each case, assume solutions ‘A’ and ‘B’ are separated by a semi-permeable membrane QuestionAnswer Solution A has 100 molecules of glucose per ml Solution B has 100 moecules of fructose per ml How will the water molecules move? There will be no net movement of water since the concentration of solute in each solution is equal Solution A has 100 molecules of glucose per ml Solution B has 75 moecules of fructose per ml How will the water molecules move? There will be a net movement of water from Solution B to Solution A until both solutions have equal concentrations of solute equal Solution A has 100 molecules of glucose per ml Solution B has 100 moecules of NaCl per ml How will the water molecules move? Each molecule of NaCl will dissociate to form a Na+ ion and a Cl- ion, making the final concentration of solutes 200 molecules per mil. Therefore, there will be a net movement of water from Solution A to Solution B until both solutions have equal concentrations of solute.

19. Osmotic Pressure Osmotic Pressure is the increased pressure resulting from osmosis. The rate of diffusion of water depends on concentration and pressure. Water will diffuse from an area of high pressure to an area of low pressure. As the pressure increases inside the membrane, the water will diffuse back across the membrane at a faster rate than before. Equilibrium is reached when: –water is still passing into the membrane because of the concentration level but… –water also passes out at the same rate because of the pressure gradient in the opposite direction.

20. Osmotic Pressure What will happen to the water levels over a period of time?

21. Passive Transport Passive transport is the movement of materials across the cell membrane without requiring energy from the cell. Materials move in the direction of the concentration gradient (high concentration to low concentration) Types of Passive transport: diffusion, facilitated diffusion, osmosis FACILITAED DIFFUSION The cell membrane contains glycoproteins which aid in the passage of certain particles across the membrane. These proteins are known as transport proteins. Certain particles that a cell needs are either too large or are not soluble in lipids to diffuse easily across the cell membrane. The transport proteins aid or facilitate these particles as they move from an area of high concentration to an area of low concentration. Transport proteins are selective because each one has a distinctive shape and charge to allow only one kind of particle to enter or leave the cell.

22. TYPES OF TRANSPORT PROTEINS USED IN FACILLITAED DIFFUSION: 1.CHANNEL PROTEINS: Also called pores. Water-filled passages through which small dissolved particles, such as ions, can diffuse. Ions pass through different pores depending on size and charge. 2.CARRIER PROTEINS: Change shape to allow different molecules to cross the plasma membrane. 3.GATE PROTEINS : Certain chemicals combine with the transport protein to signal it to “open”. Glucose diffuses into a cell this way. animation: facilitated diffusion …Passive Transport ligand

23. Scanning electron micrograph of hair cells from the bullfrog inner ear, which contain the mechanically-gated ION CHANNEL TRPA1. Sound waves bending the cilia-like projections on the hair cells of the inner ear open up ion channels leading to the creation of nerve impulses that the brain interprets as sound

24. ACTIVE TRANSPORT In active transport, cellular energy is used to change the shape of a carrier protein so that required particles move from an area of low concentration to an area of high concentration. Unlike diffusion, particles go against the concentration gradient. It is analogous to rowing a boat upstream against the current (concentration gradient).

25. Endocytosis Endocytosis is the process by which the plasma membrane of the cell surrounds the material, engulfs it and takes in the materials from the environment. THERE ARE THREE TYPES OF ENDOCYTOSIS: 1.PHAGOCYTOSIS –The transport of large particles, whole cells, or solids. –common in unicellular organisms 2.PINOCYTOSIS –The transport of solutes or fluids. –ingesting liquid droplets 3. RECEPTOR-AIDED ENDOCYTOSIS –protein receptors (in membrane) hook up with a specific molecule. Indentation in membrane forms and pinches off inside cell. Animation: Receptor-mediated endocytosis Receptor-mediated endocytosis Animation: Phagocytosis Endo and exocytosis

27. Receptor-Mediated Endocytosis:

29. Exocytosis a reverse process of endocytosis to get rid of wastes (indigestible substances) or secrete hormones. the substance isenclosed in a vesicle (surrounded by a membrane) -fuses with cell membrane -the fused membranes open to let material outside cell. Animation: Exocytosis