1. Cypress Creek Basin Clean Rivers Program Nutrient Special Study Update Report – May 16, 2007 Roy Darville

2. Study Objectives determine the quantities, seasonal patterns and source watersheds of nutrients (nitrogen and phosphorus) presently being supplied to plant and microbial populations in Caddo Lake over an annual cycle support the development of a nutrient budget to assess the trophic status (i.e., stage of reservoir eutrophication or nutrient enrichment) of Caddo Lake in comparison with similar systems within the Cypress Creek Basin and the surrounding area

3. Parameters Collected field: water temperature, dissolved oxygen, pH, specific conductivity flow: USGS & USACE internet data (except at James Bayou CR3312) nitrogen – nitrate nitrogen – nitrite total Kjeldahl nitrogen ammonia nitrogen phosphorus total organic carbon chloride

4. Study Sampling 6 sites 1) Willow Pass Spillway 2) James Bayou CR3312 3) Black Cypress SH49 4) Big Cypress SH43 5) Little Cypress US59 6) Big Cypress Below Lake O the Pines dam 19 sampling dates First sample July 13, 2006 Last sample May 6, 2007

6. Willow Pass spillway – Jan 28, 2007 5,300 cfs Caddo spillway – Jan 25, 2007 7,300 cfs

7. James Bayou CR3312 May 6, 2007 Little Cypress Bayou – US59 May 6, 2007 632 cfs

8. Black Cypress – SH49 May 6, 2007 239 cfs Big Cypress – SH43 May 6, 2007 906 cfs

9. Big Cypress – Below L.O.P. May 6, 2007 35 cfs Big Cypress – Below L.O.P. February 1, 2007 1,660 cfs

10. Sample dates