1. Microbiology for Heath Sciences Laboratory Laboratory Safety

2. BEFORE EACH LAB MUST COME PREPARED FOR EACH LAB MUST COME PREPARED FOR EACH LAB By reading the material before coming to lab By reading the material before coming to lab Knowing the dangers associated with each lab Knowing the dangers associated with each lab Knowing the proper ways of using materials provided for lab exercise Knowing the proper ways of using materials provided for lab exercise Knowing all the protective measures you must use during lab exercise Knowing all the protective measures you must use during lab exercise

3. PERSONAL PROTECTION Place personal belongings in designated areas. Keep ONLY your lab notebook on lab bench top. Place personal belongings in designated areas. Keep ONLY your lab notebook on lab bench top. Restrain long hair to prevent it from catching on fire Restrain long hair to prevent it from catching on fire Wear shoes that enclose your entire feet Wear shoes that enclose your entire feet Wash your hands with soap and water for at least 30 seconds before you begin lab and after you complete your work. Wash your hands with soap and water for at least 30 seconds before you begin lab and after you complete your work.

4. EMERGENCY PROCEDURES Know where all the exit doors are located Know where all the exit doors are located Know emergency procedure Know emergency procedure Know where the fire extinguisher and fire blanket are located Know where the fire extinguisher and fire blanket are located Notify instructor immediately for any spills or broken glassware. Notify instructor immediately for any spills or broken glassware. Report all accidents, injuries, and close calls immediately to instructor. Report all accidents, injuries, and close calls immediately to instructor. If you spill anything on your skin, immediately wash the affected area If you spill anything on your skin, immediately wash the affected area If culture gets into your eyes, immediately wash your eyes for 5 minutes If culture gets into your eyes, immediately wash your eyes for 5 minutes

5. GOOD LABORTORY ETIQUETTE Follow all instructions Follow all instructions Do not play in laboratory Do not play in laboratory No cell phones No cell phones Do not use any laboratory equipment until you are told to do so Do not use any laboratory equipment until you are told to do so Do not perform any laboratory procedure until you are told to do so Do not perform any laboratory procedure until you are told to do so

6. GOOD LABORTORY ETIQUETTE Place all contaminated tubes and plates into BIOHAZARD area Place all contaminated tubes and plates into BIOHAZARD area Keep cultures in test tube racks when working at lab bench or walking around the lab. Keep cultures in test tube racks when working at lab bench or walking around the lab.

7. GOOD LABORTORY ETIQUETTE Do not bring food or beverages into the laboratory Do not bring food or beverages into the laboratory We are working with microorganisms and you do not want them to contaminate your food or drink We are working with microorganisms and you do not want them to contaminate your food or drink You will have points deducted from your test if you are caught eating or drinking You will have points deducted from your test if you are caught eating or drinking Keep hands away from mouth and eyes. Do not apply cosmetics in the laboratory, handle contact lenses, or placing food items into mouth Keep hands away from mouth and eyes. Do not apply cosmetics in the laboratory, handle contact lenses, or placing food items into mouth Restrain loose clothing and jewelry Restrain loose clothing and jewelry No baseball caps or hats No baseball caps or hats And finally…BE ON TIME! And finally…BE ON TIME!

8. BEFORE LEAVING THE LABORATORY Clean your workbench with disinfectant at the beginning and at the end of each laboratory exercise Clean your workbench with disinfectant at the beginning and at the end of each laboratory exercise Wash your hands with soap and water for at least 1 min before you begin lab and after you complete your work. Wash your hands with soap and water for at least 1 min before you begin lab and after you complete your work.