1. 1 STCU-NATO Workshop "From Science to Business" Kyiv October 11–12, 2006 Tel.: +380 (57) 341-02-72 E-mail: patsenker@isc.kharkov.com http://www.isc.kharkov.com/old Leonid Patsenker Ph.D., Head of Department Fluorescent Probes and Labels for Biomedical Applications National Academy of Sciences of Ukraine State Scientific Institution "Institute for Single Crystals" Department of Organic Luminophores and Dyes 60 Lenin Ave., Kharkov, 61001, Ukraine

2. 2 Talk outline 1.What we offer: brief technology description 2.Stage of development 3.The market for fluorescent probes and labels 4.Who needs it? 5.Advantages of our materials 6.Our competitors and how to beat them 7.Opportunity for joint work

3. 3 Proprietary information statement The materials presented in this talk are available for licensing and joint product development None of the slides contain any confidential or proprietary information

4. 4 What We Offer Fluorescent Dyes (Luminophores) Non-fluorescent Dyes (Quenchers) Dyes for Biomedical Applications Reactive Markers (Labels) Non-reactive Markers (Probes) Classification Dyes for Microspheres

5. 5 We Offer the Life Sciences Related Products and Technologies Fluorescent probes and labels but alsoFluorescent probes and labels but also Non-fluorescent quenchers for biological and pharmaceutical researches, and biomedical assaysNon-fluorescent quenchers for biological and pharmaceutical researches, and biomedical assays Classification dyes for single or multiple encoding of microspheres used in High-throughput Screening (HTS)Classification dyes for single or multiple encoding of microspheres used in High-throughput Screening (HTS) Technologies to synthesize, produce and use these materialsTechnologies to synthesize, produce and use these materials Custom synthesis, R&DCustom synthesis, R&D 100 products

6. 6 These materials and technologies are available for licensing Fluorescent probes, labels, and non-fluorescent dyes (quenchers) for biological and biomedical applications, pharmacology and environmental pollution monitoring Fluorescent probes, labels, and non-fluorescent dyes (quenchers) for biological and biomedical applications, pharmacology and environmental pollution monitoring Classification dyes for beads Classification dyes for beads Other luminescence-based materials Other luminescence-based materials Technologies to synthesize, produce and use these materials Technologies to synthesize, produce and use these materials Some of Our Existed Patents and Patent Applications: 1.PCT WO 2003/087052 A3 (2003) 2.US Patent 6538129 (2003) 3.US Patent Application 2004/0166515 A1 (2004) 4.US Patent Application 2003/0235846 A1 (2003) 5.US Patent Application 2005/0202565 A1 (2005) Stage of Development

7. 7 A Sample Page @ Online Product Catalog http://www.isc.kharkov.com/oldhttp://www.isc.kharkov.com/old

8. 8 MARKET for Fluorescent Probes & Labels: Biology, Medicine, Pharmacology, and Environmental Pollution Monitoring Biological Imaging Clinical Diagnostics High-throughput Screening (HTS) Real-time PCR Based Applications Cellular and Molecular Biology Cytology Immunology Genomics Proteomics Enzymatic Drug Screening Photodynamic Therapy Environmental (chemical and bacterial) pollution monitoring

9. 9 Who needs our markers? The potential application of fluorescent probes, labels, and classification dyes is very versatile. Therefore the market for these materials is huge Any company using fluorescence as a read-out parameter can be interested in our products These are organizations working in the life-science market: Biomedical companies Clinical laboratories Environmental monitoring laboratories Academic researchers in the biological, chemical and medical fields Fluorescent dyes for biomedical applications are a multi-billion dollar business!

10. 10 Our products have been tested at: Our products have been tested at: SETA Biomedicals (USA) SETA Biomedicals (USA) Perkin Elmer, Inc. (USA) Perkin Elmer, Inc. (USA) Tecan (Austria) Tecan (Austria) ReaMetrix, Inc. (USA) ReaMetrix, Inc. (USA) Dako (Denmark) Dako (Denmark) Luminex Corp. (USA) Luminex Corp. (USA) LJL Biosystems (USA) LJL Biosystems (USA) Molecular Devices (USA) Molecular Devices (USA) ISS (USA) ISS (USA) Avanti Polar Lipids, Inc. (USA) Avanti Polar Lipids, Inc. (USA) Edinburgh Instruments (Great Britain) Edinburgh Instruments (Great Britain) Becton Dickinson (USA) Becton Dickinson (USA) Pierce Biotechnology (USA) Pierce Biotechnology (USA) Glen Research Corp. (USA) Glen Research Corp. (USA) Potential customers: Abbot (USA) Abbot (USA) Bayer (Germany) Bayer (Germany) Pfizer (USA) Pfizer (USA) GE Healthcare (Amersham) (USA) GE Healthcare (Amersham) (USA) Invitrogen (Molecular Probes) (USA) Invitrogen (Molecular Probes) (USA) Glaxo Smith Kline (USA) Glaxo Smith Kline (USA) CIS Bio International (France) CIS Bio International (France) Alpha Diagnostics International (USA) Alpha Diagnostics International (USA) Dakota Technologies, Inc. (USA) Dakota Technologies, Inc. (USA) IQ Products (The Netherlands) IQ Products (The Netherlands) GeneFocus (Biomedical Photometrics Inc.) (Canada) GeneFocus (Biomedical Photometrics Inc.) (Canada) Many others... Many others...

11. 11 We offer biomedical markers for a wide spectral range, from the ultra-violet to the near-infrared As compared to materials from other producers our markers have certain advantages in most all characteristics

12. 12 New markers are excitable not only with red diode lasers but also with blue diode lasers, LEDs, and UV light New markers show noticeable increase of fluorescence intensity and longer fluorescence lifetimes in presence of protein Advantages of Our Markers Compatibility with Various Excitation Light Sources Response for Microenvironment

13. 13 New reactive labels demonstrate higher brightness when bound to protein as compared to e.g. Cy5 of GE Healthcare New markers can be made pH-sensitive Advantages of Our Markers High Brightness pH Sensitivity

14. 14 Decay of brightness of a biological image High Photostability Biological Imaging Advantages of Our Markers New markers are much more photostable compared to those from other producers, which is particularly important to obtain higher-stable biological images

15. 15 Advantages of Our Markers Fluorescent Probes and Reactive Labels Effective Quenchers (Real Dark Dyes)

16. 16 Competition Invitrogen (Molecular Probes, USA) GE Healthcare (Amersham, USA) Leading Competitors: Dyomics GmbH (Germany) Active Motif Chromeon GmbH (Germany) Several small companies Other Competitors:

17. 17 Our biomedical materials have better characteristics: Higher brightness Longer luminescence lifetimes, which make them useful for the FLT and polarization based assays Higher photostability Our materials are cheaper How will our materials beat the competitors?

18. 18 Competitive Matrix Characteristic Our Marker Seta-640-di-NHSInvitrogen (Molecular Probes) Alexa Fluor 647 GE Healthcare (Amersham) Cy5 NHS ester Absorption / Emission Wavelength 648 / 656 nm 650 / 668 nm 648 / 664 nm Quantum Yield of BSA Conjugate65%42%25% Luminescence Lifetime (Free / Bound to BSA) 0.7 / 3.0 ns 1.1 / 1.2 ns 1.0 / 1.2 ns Applicability for the polarization and FLT assaysYesNoNo Photostability90%80%60% Price $125 / 1 mg $2,500 / 50 mg $212 / 1 mg $4,300 / 50 mg $222 / 1 mg $4,455 / 50 mg

19. 19 Competitive Matrix Characteristic Our Marker SeTau-380- NHS Our Marker SeTau-425- NHS InvitrogenDPHInvitrogenNBD Absorption / Emission Wavelength 381 / 480 nm 425 / 545 nm 360 / 430 nm 470 / 530 nm Quantum Yield (Free / Bound to BSA) 56% / 44% 39% / 16% 0% / 80% 0% / 32% Fluorescence in waterYesYesNoNo Luminescence Lifetime (Free / Bound to BSA) 33 / 31 ns 26 / 13 ns 4 / 8 ns 5 / 10 ns Price per 1 mg$70$80$78$180

20. 20 Opportunities for Joint Work Search for the continuous customers of our products and technologies... Search for the continuous customers of our products and technologies... Collaboration with companies which are interested in further testing, licensing and commercialization of our materials and technologies... Collaboration with companies which are interested in further testing, licensing and commercialization of our materials and technologies... Collaboration with companies which are interested in further joint development of our materials towards making of new "Marker Specific Biological Species" conjugates such as fluorescently labeled peptides, oligonucleotides, proteins, antibodies, and antigens but also development of kits and assays based on our markers... Collaboration with companies which are interested in further joint development of our materials towards making of new "Marker Specific Biological Species" conjugates such as fluorescently labeled peptides, oligonucleotides, proteins, antibodies, and antigens but also development of kits and assays based on our markers...

21. 21 STCU Project U111 STCU Project U111 Red and NIR Absorbing and Emitting Fluorescent Probes and Labels for Use in Biological and Biomedical Research 2004 2006 Commercialization STCU 3804 Fluorescence Lifetime-based Probes and Labels for Biomedical Assays and Bacterial Pollution Monitoring Submitted STCU Project 3795 Improving Multiplexed Analysis Systems for Environmental Pollution Monitoring and Medical Diagnostics Using Novel Classification Dyes Submitted

22. 22 Leonid Patsenker Ph.D., Head of Department Tel.: +38 (057) 341-02-72 Fax: +38 (057) 340-93-43 E-mail: patsenker@isc.kharkov.com http://www.isc.kharkov.com National Academy of Sciences of Ukraine State Scientific Institution "Institute for Single Crystals" Department of Organic Luminophores and Dyes 60 Lenin Ave., Kharkov, 61001, Ukraine Contact Information