1. Topics 1.Introduction 2.System setup 3.Sleep stages 4.Analysis 5.Video Function Sleep Analysis Program SleepSign for Animal KISSEI AMERICA, INC.

2. 1. Introduction A fruit of a joint-research with the Osaka Bioscience Institute (Osaka, Japan) First version developed in 1998 by KISSEI COMTEC (ISO9001 registered) Introduced to USA in 2001 KISSEI AMERICA, INC.

3. 1. Introduction Benefits Reduce time and labor for sleep scoring Quick and sophisticated report generation KISSEI AMERICA, INC.

4. 2. System setup Multifunction A/D card ADA16-32/2(CB)F BNC analog input table ATP-32F Multifunction A/D board ADA16-32/2(PCI)F ADC interface for Desktop ADC interface for Laptop Analog output of an amplifier 96pin half pitch connector cable PCB96PS-0.5P 32ch BNC analog input table ATP-32F 32ch Import directly Data import - Biopic Acknowledge data file - DSI Dataquest A.R.T. data file - EDF (European Data Format) file KISSEI AMERICA, INC.

5. 3. Sleep stages Wakefulness NonREM sleep REM sleep stages EEG EMG High frequency (4-10Hz) Low amplitude ActiveNot active Low amplitude Low frequency (0.5-4Hz) High amplitude High frequency (4-10Hz) Theta wave Delta wave Light sleepDeep sleep

6. 4. Analysis Automatic scoring Review / Modification (1) Stage Graph(2) Vigilance State Parameters(3) Average FFT Amount of SleepQuality of SleepSleep-Wake transition pattern Generate reports KISSEI AMERICA, INC.

7. 4. Analysis KISSEI AMERICA, INC. Automatic Scoring

8. (1) Stage Graph % Time spent in each states 4. Analysis KISSEI AMERICA, INC. Amount of Sleep

9. (2) Vigilance State Parameters A list of each sleep bouts & summary of user defined time bin 4. Analysis KISSEI AMERICA, INC. Sleep-Wake transition pattern

10. (3) Average FFT EEG spectral power averaged by each sleep stages and time bin 4. Analysis KISSEI AMERICA, INC. Quality of Sleep

11. 5. Video Function Video monitor along with EEG, EMG. Enhance accuracy of sleep scoring Behavioral analysis KISSEI AMERICA, INC.

12. 5. Video Function KISSEI AMERICA, INC. MPEG encoder board MVR D2200V RCA cable Multiplexer Camera SleepSign for Animal Analog output of an amplifier 16ch (8) RCA jack to BNC pin adapter Desktop only Multifunction A/D board ADA16-32/2(PCI)F 96pin half pitch connector cable PCB96PS-0.5P BNC analog input table ATP-32F

13. 5. Video Function Video file Import KISSEI AMERICA, INC.