1. LFA = little fire ant Photo © Alex Wild

2. If you’ve been stung by ants, they were probably Tropical Fire Ants, not LFA Tropical Fire Ant Widespread Live in sunny, dry, open areas like parks Nests in the ground have distinct openings Size: 1/4 inch; about 1/3 the size of a penny Little Fire Ants Present on Hawaii island, limited elsewhere Tend to live in trees and plants in moist, shady areas Nests have no distinct opening 1/16 inch; as long as a penny is thick

3. This 7 minute video is a good summary of the little fire ant problem and why we are so concerned. http://www.youtube.com/watch?v=mJ8TQ_ASsYQ http://www.youtube.com/watch?v=mJ8TQ_ASsYQ Populations reach 244 million ants/hectare = about 11 million ants/5000 sf lot What are Little Fire Ants (LFA)? Little fire ants attracted to peanut butter on half of a chopstick

4. Little Fire Ants stings are unavoidable in forests, fields & homes… live in trees, on the ground,

5. stings are small and painful and create itchy rashes Little Fire Ants

6. Eye stings result in clouded cornea and blindness in animals

7. Little Fire Ants weaken plants by farming insects like aphids and mealy bugs

8. www.littlefireants.com Little fire ants are originally from tropical forest regions in South America (orange area) They have now spread to tropical areas worldwide (black dots) LFA one of the “100 Worst Invasive Species”

9. www.littlefireants.com From: Foucaud, J. Orivel, J. Loiseau, A. Delabie, J.H.C. Jourdan, H. Konghouleux, D. Vonshak, M. Tindo, M. Mercier, J. Fresneau, D. Mikissa, J. McGlynn, T. Mikheyev, A.S. Oettler, J. and Estoup, A. (2010). Worldwide invasion by the little fire ant: routes of introduction and eco-evolutionary pathways. Evolutionary Applications. 1-13 Tracing LFA genes (follows plant/ag trade patterns)

10. Current LFA Infestation

11. 2013 From infested areas—East Hawai ʻ i (maybe Oahu?) 1999—1 site) 2013—10+ sites, 2014—1 site 3-10 yrs old? 2009—1 site eradicated, 2013—1 site 2014—3 sites 1999 2013—4+ sites Plants Cut flowers & ti leaves Fruit & produce Planting materials like hapu`u, greenwaste Non-ag products--cinder LFA colonies are moving in: LFA colony in macadamia nut shell

12. Ants have been around for at least 130 million years Ants found in 96 million year old amber look pretty much the same as today’s ants; they already had a complex social structure There are tens of thousands of species of ants in the world Ants: the nearly perfect beast In order to control ant populations, we must understand them

13. How the Queen Gets her Meals carbohydrates (sugar food) Proteins (animal-based food) Lipids (fatty food) Other workers Nurse workers Other worker Queen attendants larvae Foraging worker (picks up carbs & lipids, feeds to workers) Nurse worker 4 th instar larvae Queen attendants QUEEN Foraging worker (picks up proteins, feeds to nurse workers)

14. A little about ant colony structure: The colony is a super-organism – a group of animals that work together with one goal: to support the queen A queen – the only member of the colony that produces offspring* (LFA colonies can have more than 1 queen, but no colony can exist without at least 1 queen) Within the worker caste, some workers act as guards, some are hunter-gatherers or farmers, some are nurses to look after the larvae, others are attendants to look after the queen Foraging workers – all sterile daughters of the queen (these are the ants we usually see—workers are easily replaced) Males – produced and utilized as needed for reproduction Larvae – infant workers, new queens and males which are cared for by the nurse workers

15. But how can we hope to win? The queen is the answer—without a queen, the colony dies We need to use the right ant “baits”—attractive food items with slow-acting insecticide or insect growth regulator—near colonies, foraging workers will unknowingly help us feed it to the queen But we can’t kill them if we can’t find them…we need everyone’s help

16. 10+ nurseries/garden shops that sold infested hapu`u being treated every 6 weeks for 1 year; with monitoring for 1 additional year after Ongoing: HDOA & OISC have a list of nearly 175 nurseries/plant businesses prioritized for baseline survey— very small populations of LFA are still being found, treated April 2014: Found on Waimanalo unmanaged lands; site treated 8x over 1 year, will be surveyed quarterly for 3 years June 2014: Mililani Mauka resident saw LFA media and tested yard for ants, turned in sample to HDOA. 20 properties & greenspace treated 8x, will be surveyed quarterly for 3 years The good news: These ants are easy to kill and even eradicate (there are a variety of products available)—if we find them before they form large populations! Oahu Situation Report

17. Spot the Ant, Stop the Ant 2015 Oahu schools/public ant survey locations to date (9/15)…has your neighborhood or community been surveyed?

18. The ONLY people that can relax are living at those 20 Mililani Mauka properties. Everyone else should test their yard for LFA! 3 minute video on how to test for LFA: How to test for Little Fire Ant-HD.mp4 (available online: http://vimeo.com/97558997)http://vimeo.com/97558997

19. Take 1 zip top bag & 5-15 chopsticks (or craft sticks) home. Plan survey for 1 hour in morning or evening (do not survey when raining) Spot the Ant, Stop the Ant: Testing for LFA Smear a tiny amount of peanut butter on one end of each chopstick. Scrape off almost all of the peanut butter back into the container. (use mayonnaise if you are allergic). Remember, more is NOT better, its just messier. Place in shady, moist areas, in and around plants, pet food sites, garbage cans, etc.

20. LFA Survey As you place your chopsticks, draw an easy diagram of your survey site (yard, park or school). Number the approximate locations of each chopstick so you can find them Wait 45 min-1 hour (not longer!) Label your zip top bag with your name and address using permanent marker Check each chopstick for ants—if there are ants, carefully pick up the chopstick and place it in the zip top bag, then seal it Place the bag in the freezer overnight to kill the ants before bringing them to school! At school we will look at the ants under the microscopes (or your teacher will send to the HI Department of Agriculture)

21. In September, Oahu residents can participate in the KSSK/IHeartMedia Stop the Ant Photo Contest SPOT the giant Little Fire Ant around Oahu, snap a photo, and post your photo on www.StopTheAnt.org, or tag us in your Instagram photo with @stoptheant and be sure to use #StopTheAntOahu. Spot the Ant, Stop the Ant Month on Oahu

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