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EDI - mRNAEDI + mRNA EDI pre-mRNA RT-PCR NH2 COOH EDI EDII IIICS 20 splicing isoforms PAGE EDI Minigene EDI Fibronectin Muñoz et al. Figure 1 ab cd.

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Presentation on theme: "EDI - mRNAEDI + mRNA EDI pre-mRNA RT-PCR NH2 COOH EDI EDII IIICS 20 splicing isoforms PAGE EDI Minigene EDI Fibronectin Muñoz et al. Figure 1 ab cd."— Presentation transcript:

1 EDI - mRNAEDI + mRNA EDI pre-mRNA RT-PCR NH2 COOH EDI EDII IIICS 20 splicing isoforms PAGE EDI Minigene EDI Fibronectin Muñoz et al. Figure 1 ab cd

2 EDI P hCMV*-1 7 tet operators pUHC-EDA tTA-Sp1 tetR aa 1 207 Sp1 Gln rich aa 268 499 P CMV tTA-VP16 tetR aa 1 207 VP16 acidic domain aa 363 490 P CMV Muñoz et al. Figure 2 a b

3 - amanitin resistant pol II (large pol II subunit) endogenous pol II - amanitin (24 hr) RNAPII - am R Endogenous pol II EDI - am WT o mutant EDI Hep3B (human hepatoma) Asn 792 Asp Muñoz et al. Figure 3 reporter minigene

4 Muñoz et al. Figure 4

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