1. Utilizing Science & Technology and Innovation for Development Study the interaction of nanoparticles with proteins Marriott Hotel- Amman, August 13th, 2015

2. Project Team Dr. Mayyas Al-Remawi Taif University/KSA Dr. Diya Alsafadi Royal Scientific Society/Jordan

3. Brief Description The development in the nanotechnology in the last decade has dramatically widened the prospect of using nanoparticles (NPs) with unique properties.

4. - Due to size, nanoparticles more easily taken up by the human body and can cross biological membranes, cells, tissues and organs more efficiently than larger particles. - Less than 100nm enter cell, less than 40nm enter nucleus, less than 35nm pass Blood Brain Barrier. Brief Description Problem  Disposal and environmental fate?  Potential toxicity & Interactions with living cells

5. Nanoparticle-Protein Corona  Nano- particles in physiological fluids interact initially with the proteins.  Formation of the corona is a dynamic, competitive process

6. Justifications 100 nm Chitosan Nanoparticles Used as drug delivery Silver Nanoparticles Used in electronics, bio- sensing, clothing, paints, cosmetics and medical devices BSA & HSA

7. Objectives Evaluate the biological hazard of nanoparticles by investigating its interaction with proteins. Assess the effect of silver and chitosan nanoparticles on serum proteins using spectroscopic techniques.

8. Scope of work/Duration Estimated Budget Scope of work: Nanosafety and nanomedicine sciences. Duration: 24 months Estimated Budget : 210,000 JD

9. Methodology of Implementation Nanoparticle synthesis Protein Corona Functional impacts / toxicity In vivo effects Dispersion and characterisation

10. Expected output Deliver medicine/ treatment to new sites in the body and cure people. Avoid harming people through the application of nanotechnology. By understanding the bionanointeractions, we will be able to

11. Impact The project will provide a significant contribution to the implementation of community environmental policy and legislation in the field of nanosafety and nanotoxicity. The outcomes and recommendations of the project will be sent to Ministry of Environment, Ministry of Health and JFDA.

12. Sustainability Institutional sustainability  Build a life communications plan between the academy and industry.  Prepare and market short courses related to project out comes in collaboration with public and private sectors. Policy level sustainability Through proper channels we will create communications with the policy makers to approve legislations to encourage public and industrial sector to be aware for the using of NPs.

13. Action Plan Q1Q2Q3Q4 kkkkk NP- Protein Corona synthesis &characterization In vivo effects Chitosan NPs synthesis SEM, TEM, Zeta Potential Ag-NPs synthesis XRD, AA and ICP NP-synthesis & characterization Functional impacts / toxicity Royal Scientific Society Taif University Chitosan NP-protein corona Ag-NP-protein corona UV.VIS,CD and FTIR Fluorescence spectroscopy, SDS-PAGE & Gel filtration In vivo effects Functional impacts / toxicity

14. Thank You