1. pp65-specific all B: CD8 T-cells CD8/pp65 T cells per ml blood A: CD8 T-cells Indicated memory subset as a proportion of the reference T-cell subset FIG. S1. The size of pp65-specific responses determines the memory T-cell subset distribution within that response but also within the entire T-cell compartment. Scatter plots show the correlation of CD8/pp65 absolute response sizes (cells per ml of blood) versus the memory subset distribution within these responses (A) or in the entire CD8 T-cell compartments (B) in 39 donors. Significant correlations with R S >0.50 are highlighted bold. Counts are shown on a linear scale for better visualization of subtle changes. R S =-.387, p=.015 R S =.378, p=.018 R S =.026, ns R S =.010, ns R S =.318 p=.049 R S =.030, ns R S =-.215, ns R S =-.610, p=.000 T Naive T EMRA T CM T EM

2. Indicated memory subset as a proportion of the reference T-cell subset R S =-.302, ns R S =.440, p=.006 R S =-.142, ns R S =.304, ns CD4/pp65 T cells per ml blood R S =.573 p=.000 R S =-.489, p=.002 R S =-.230, ns pp65-specific all A: CD4 T-cellsB: CD4 T-cells R S =-.530, p=.001 FIG. S2. The size of pp65-specific responses determines the memory T-cell subset distribution within that response but also within the entire T-cell compartment. Scatter plots show the correlation of CD4/pp65 absolute response sizes (cells per ml of blood) versus the memory subset distribution within these responses (A) or in the entire CD4 T-cell compartments (B) in 38 donors. Significant correlations with R S >0.50 are highlighted bold. Counts are shown on a linear scale for better visualization of subtle changes. T Naive T EMRA T CM T EM

3. T cells with 4 activation markers T cells with 3 activation markers T cells with 2 activation markers T cells with 1 activation marker T cells without activation markers B IFN-g CD4 T-cells IFN-g A TNF-a CD8 T-cells TNF-a FIG. S3. Polyfunctional T-cells display larger quantities of each activation marker. Representative dot plots show the expression levels (MFI) of IFN- and TNF- of pp65/CD8 and pp65/CD4 T-cell subsets displaying different numbers of activation markers after stimulation with pp65 for 16 hours. T-cells expressing no (black), one (red), two (blue), three (green) or four (orange) activation markers are shown. Dot plots show log mean fluorescence intensities.