1. Chapter 14 Pathology

2. Definitions! Pathology – study of disease Etiology – cause of disease Pathogenicity – how a pathogen overcomes host defenses to produce disease Pathogenesis – development and progression of disease Epidemiology – occurrence and spread of disease

3. Symbiosis A relationship between two organisms Mutualism – both benefit Commensalism – one benefits, other is unaffected Parasitism – one benefits, other is harmed

4. Normal Flora in Humans Resident flora – permanent microbes in the body –Most are commensals or mutualistic Transient flora – temporary microbes in the body

5. Symbiosis – Special Cases Microbial antagonism – competition between microbes. Normal flora outcompete pathogens in our bodies. This is a form of mutualism between human and normal flora. Opportunism – disease caused when organism is in a new environment, the host is immunocompromised, or normal flora disturbed –E.g. E. coli in UTI, Pneumocystis in immunodeficient patients, overuse of antibiotics

6. Koch’s Postulates – Proves Etiology

7. Summary of Koch’s Postulates 1.The same pathogen must be present in every case of disease. 2.The pathogen must be able to be isolated and cultured in pure media. 3.The cultured pathogen from step 2 must be able to cause disease again. 4.The same pathogen must be able to be isolated from the organism given disease.

8. Exceptions to Koch’s Postulates The pathogen may not be able to be cultured in pure media (e.g. viruses, Rickettsia). Diseases may have multiple causes (e.g. nephritis, UTI) One pathogen causes multiple diseases/symptoms (e.g. Mycobacterium tuberculosis affects lung, brain, kidney, skin and bones)

9. Pathogenicity in Bacteria Adherence – binding to host cells Penetration – entry into host cells Toxins – compounds that harm the host

10. Adherence Bacterial structures (pili, fimbriae, capsules, slime layers) allow attachment to host Specific proteins such as adhesins may be used for specific attachment to receptors on the host

11. Biofilms Mass of pathogens in cooperative adherence. First organisms that attach secrete materials that assist others to attach and colonize E.g. dental plaques on teeth.

12. Penetration Endocytosis – adherence can trigger endocytosis in host cell. Invasins may be involved that help rearrange cytoskeleton to facilitate entry and intracellular movements.

13. Penetration Tissue degradation – enzymes secreted that dissolve barriers. E.g. hyaluronidase breaks down hyaluronic acid, a sugar that holds cells together. This is the cause of gangrene.

14. Penetration Clotting –Coagulase produced by staph allows clots to form, protecting the colony. –Streptokinase can dissolve clots to free pathogen so they can spread.

15. Toxins Endotoxins –Produced by Gram- –Part of the LPS –Nonspecific –Low toxicity (fever, aches) –Released when bacteria die or divide Exotoxins –Produced mostly by Gram+ –Secreted –Specific targets –High toxicity

16. Exotoxins Types Hemolysins – destroy red blood cells. Neurotoxins – attacks nervous system. Superantigens – provoke intense immune response.

17. Pathogenesis Types of Infections –Duration and severity –Placement of infection –Sequence Disease Progression –Stages of infection

18. Severity and Duration of Disease Acute – quick (weeks) but severe –E.g. flu, ebola Chronic – long lasting (months-years) and continuous –E.g. tuberculosis Latent – has an inactive phase –E.g. HIV

20. Placement Local – confined to one area Focal – localized to one area but toxins/pathogens can affect other areas Systemic – affects entire body

21. Sequence of Infections Primary infection – the first infection of a healthy person Secondary infection – the second pathogen. Usually opportunistic –Superinfection – a type of opportunistic infection when the normal flora is destroyed.

22. Disease Progression 1.Incubation – infection, no symptoms 2.Prodromal – early/mild symptoms 3.Invasive – most acute and dangerous. Acme is the peak 4.Decline – begin recovery, symptoms subside 5.Convalescence – fully recovered, body regains strength

23. Disease Progression