1. Exposure and Effects Pilot Study Small Fish Mercury Project Ben Greenfield, Letitia Grenier, Andrew Jahn, Seth Shonkoff, Joy Andrews, Esther Letteny, Mark Sandheinrich

2. EEPS Small Fish Hg Project Goals Goals Characterize food-web mercury at finer spatial and temporal scales than sport fish studies Characterize food-web mercury at finer spatial and temporal scales than sport fish studies Detect regional trends in bioaccumulation of Hg related to wetland restoration Detect regional trends in bioaccumulation of Hg related to wetland restoration Identify differences in pattern between benthic and pelagic food webs Identify differences in pattern between benthic and pelagic food webs

3. EEPS Small Fish Hg Project Design Design 8 sites from South Bay to Suisun Bay 8 sites from South Bay to Suisun Bay 4 sites near restoration projects 4 sites near restoration projects 4 index sites near extant marshes 4 index sites near extant marshes Additional samples in deep water Additional samples in deep water Sample benthic and pelagic species at each site Sample benthic and pelagic species at each site Use related types of species to create data sets comparable across a wide range of salinities Use related types of species to create data sets comparable across a wide range of salinities Topsmelt/Mississippi (inland) silversides (Atherinopsidae) Topsmelt/Mississippi (inland) silversides (Atherinopsidae) Arrow goby, cheekspot, and Shimofuri goby (Tridentiger) Arrow goby, cheekspot, and Shimofuri goby (Tridentiger) Bay goby from deep water Bay goby from deep water

4. Design Design Four composites per species per site - allow statistical comparison Four composites per species per site - allow statistical comparison 5-10 individuals/composite 5-10 individuals/composite Minimize covariates by using consistent sizes Minimize covariates by using consistent sizes EEPS Small Fish Hg Project

5. Collaboration and leverage Collaboration and leverage Analytical lab - UW LaCrosse River Studies Center Analytical lab - UW LaCrosse River Studies Center Additional samples for individual Hg analysis by CSU - East Bay (Joy Andrews) Additional samples for individual Hg analysis by CSU - East Bay (Joy Andrews) Extra samples collected using clean techniques for trace organics analysis Extra samples collected using clean techniques for trace organics analysis Looking for funding for analysis Looking for funding for analysis Bay Gobies from IEP trawl surveys Bay Gobies from IEP trawl surveys EEPS Small Fish Hg Project

6. Coordination with other monitoring Coordination with other monitoring Geographic Geographic EEPS samples bay edge - South, Central and North Bay EEPS samples bay edge - South, Central and North Bay South Bay Salt Pond Hg project samples inside marshes, ponds, and sloughs South Bay Salt Pond Hg project samples inside marshes, ponds, and sloughs CBDA Fish Mercury Project - North Bay, more freshwater focus CBDA Fish Mercury Project - North Bay, more freshwater focus Species Species Intent to produce data set as comparable as possible across region Intent to produce data set as comparable as possible across region EEPS Small Fish Hg Project

8. Treatment Reference Bay Goby

9. EEPS Small Fish Hg Project Treatment Reference Bay Goby