1. Photosynthesis: The Reaction

2. Recap…….. 6 CO 2 + 6 H 2 0  C 6 H 12 O 6 + 6 O 2 http://www.youtube.com/watch?v=lqEDz2vfhp Q&feature=related

3. Leaf Structure Most photosynthesis occurs in the palisade layer. Gas exchange of CO 2 and O 2 occurs at openings called stomata surrounded by guard cells on the lower leaf surface. Palisade Spongy

4. Chloroplast Structure Inner membrane called the thylakoid membrane. Thickened regions called thylakoids. A stack of thylakoids is called a granum. (Plural – grana) Stroma is a liquid surrounding the thylakoids.

5. Chloroplast Structure Inner membrane called the thylakoid membrane. Thickened regions called thylakoids. A stack of thylakoids is called a granum. (Plural – grana) Stroma is a liquid surrounding the thylakoids.

6. Pigments Chlorophyll A is the most important photosynthetic pigment. Other pigments called antenna or accessory pigments are also present in the leaf. o Chlorophyll B o Carotenoids (orange / red) o Xanthophylls (yellow / brown) These pigments are embedded in the membranes of the chloroplast in groups called photosystems.

8. Photosynthesis Occurs in 2 Steps Light Reaction –Light reactions are the “photo” part of photosynthesis. Light is absorbed by pigments –Energy from light drives the reaction Light Independent Reaction or Dark Reaction The Calvin Cycle the “synthesis” part of photosynthesis. Trapped energy from the sun is converted to the chemical energy of sugars. Doesn’t need direct light energy, but it does need the high-energy products from the Light Reaction

9. Photon Water-splitting photosystem NADPH-producing photosystem ATP mill Two types of photosystems cooperate in the light reactions

10. Light Reactions Light-dependent reactions occur on the thylakoid membranes. o Light and water are required for this process. o Energy storage molecules are formed. (ATP and NADPH) o Oxygen gas is made as a waste product.

11. Light Reaction Drawing first…….

13. Light Reaction Now words……. STEP 1 - Light energy is absorbed by chlorophyll in the thylakoid membranes of the chloroplast STEP 2 - In Photosystem II the electrons become energized or “excited”.

14. STEP 3 - The excited electrons are passed down a series of molecules called the electron transport chain. STEP 4 - To replace the electrons that are lost, some are stolen from water. This breaks the water molecule apart like this: 2H 2 O  4H + + O 2 STEP 5 - As electrons move down the chain, their energy is used to make ATP (a high- energy molecule)

15. 6.STEP 6 - The electrons now go to Photosystem I. Light energizes new electrons in PI and they combine with NADP+ to make NADPH (another high-energy molecule).

16. Reactants used during the light reaction: 1.Water 2.Also used sunlight

17. The final products of the light reaction 1.ATP 2.NADPH 3.O 2

18. Review Light Reaction Light Reaction – Where does this happen: – Needs: – Makes:

21. That’s only HALF of the Photosynthesis Reaction…… light H 2 O ---------------→ O 2 + ATP + NADPH chlorophyll

22. Dark Reactions Dark reactions (light-independent) occur in the stroma. This reaction is ENZYMATIC not ELECTRONIC Doesn’t ACTUALLY happen in the dark…. o Carbon dioxide is “fixed” into the sugar glucose. o ATP and NADPH molecules created during the light reactions power the production of this glucose.

23. Why fix CO2? By fixing CO2, energy is stored in organic compounds

24. Dark Reaction 3 steps: – Carboxylation – Reduction – Regeneration

26. *It takes 3 turns of the Calvin Cycle to release 1 PGAL* * 2 PGAL = 1 Glucose*

28. Dark Reaction Write it: Step 1: CO2 (1C) is added to RuBP (5C) making an unstable 6C molecule that is quickly converted to 2 PGA (3C each) molecules. Catalyzed by rubisco Step 2: PGA (3C) is reduced to PGAL (3C) with the help of NADPH and ATP. 2 PGA (3C)  2 PGAL (3C) Step 3: PGAL is converted to RuBP with the help of ATP or “siphoned” off to create glucose (later)

29. Dark Reaction Step 4: REPEAT 2 more times At third turn: 1 PGAL is released. When 2 PGALs combine, glucose is created

30. Dark Reaction What we need from the Light Reaction: – CO2 – NADPH – ATP

31. Dark Reaction Products: – SUGAR

32. The Calvin cycle makes sugar from carbon dioxide o ATP generated by the light reactions provides the energy for sugar synthesis o The NADPH produced by the light reactions provides the electrons for the reduction of carbon dioxide to glucose Light Chloroplast Light reactions Calvin cycle NADP  ADP + P The light reactions convert solar energy to chemical energy o Produce ATP & NADPH AN OVERVIEW OF PHOTOSYNTHESIS

33. Dark Reaction Build it….

34. Photosynthesis Act it….

35. 1,2,3 http://www.youtube.com/watch?v=yrQzEw9x Y5k&feature=related http://www.youtube.com/watch?v=yrQzEw9x Y5k&feature=related Rap http://www.youtube.com/watch?v=pdgkuT12 e14&feature=related