1. Introduction to Antibiotics Prof. Azza ELMedany Pharmacology Department

2. Definition of Antibiotics Chemical substances produced by various microorganisms ( bacteria, fungi, actinomyctes) that have the capacity to inhibit or destroy other microorganisms. Now a day they are chemically synthesized. They either kill bacteria(bactericidal) or keep more bacteria from growing(bacteristatic). Antibiotics will not cure infections caused by viruses.

3. CLASSIFICATION OF ANTIBIOTICS ACCORDING TO MECHANISM OF ACTION INHIBITION OF CELL WALL SYNTHESIS e.g. Penicillins INHIBITION OF CELL WALL SYNTHESIS e.g. Penicillins INHIBITION OF PROTEIN SYNTHESIS e.g. Macrolides INHIBITION OF PROTEIN SYNTHESIS e.g. Macrolides INHIBITION OF NUCLEIC ACID SYNTHESIS e.g. Quinolones. INHIBITION OF NUCLEIC ACID SYNTHESIS e.g. Quinolones.

4. According to spectrum Narrow spectrum, e.g.: Narrow spectrum, e.g.: penicillin G, aminoglycosides penicillin G, aminoglycosides Broad spectrum, e.g.: Broad spectrum, e.g.: ampicillin, amoxicillin ampicillin, amoxicillin

5. Choice of Antibiotic A)Clinical diagnosis (e.g. syphylis) B)Microbiological information C)Pharmacological consideration

6. B)Bacteriological informations Advantages  T T T The exact antibiotic to be used  T he most effective and reject the one with little or no activity he least toxic he cheapest

7. Disadvantages  O ccasionally these tests do not parallel in vivo sensitivity  d o not take in consideration certain sites of infection  s ome bacteria cannot be cultivated or take time to grow ( e.g. M. Leprae, M. Tuberculosis )  B acteriological services are not available at all hospitals

8. Choice of Antibiotics(cont.) C)Pharmacological consideration 1. Site of infection 2. Host factors a) Immune system e.g. Alcoholism, diabetes, HIV, malnutrition, advanced age- (higher than usual doses or longer courses are required ). b) Genetic factors e.g. Patients with G-6-PD deficiency treated with sulfonamides and chloramphenicol (Hemolysis )

9. Choice of Antibiotics ( Cont.) c) Pregnancy and Lactation c) Pregnancy and Lactation Aminoglycosides- ( hearing loss) Aminoglycosides- ( hearing loss) Tetracyclines- (bone deformity) Tetracyclines- (bone deformity) d) Age of the patient d) Age of the patient e.g. Grey baby Syndrome- (chloramphenicol ) e.g. Grey baby Syndrome- (chloramphenicol ) e) Renal function e.g. Aminoglycosides ( renal failure ) e.g. Aminoglycosides ( renal failure ) f)Liver function e.g. Erythromycin( hepatic failure ) e.g. Erythromycin( hepatic failure ) g) Poor perfusion e.g. Lower limbs of diabetics e.g. Lower limbs of diabetics

10. Choice of Antibiotics ( Cont.) 3. Drug Allergy 4. Drug safety Chloramphenicol ( a plastic anaemia) Chloramphenicol ( a plastic anaemia) Fluoroquinolones in children&Preg. Fluoroquinolones in children&Preg. ( tendon damage ) ( tendon damage ) 5. The cost of therapy 5. The cost of therapy

11. Bacterial Resistance Bacterial Resistance Mechanism of Bacterial resistance:  Inactivation of antibiotics by enzymes produced by bacteria  Reduced bacterial permeability to antibiotics  Bacteria develops an altered receptor for the drug  Bacterial Mutation

12. Prevention of Resistance *Use antibiotics only when absolutely required *Use antibiotics in adequate dosage for sufficient period of time Not too brief therapy Not too brief therapy Not too prolonged therapy Not too prolonged therapy *Combination of antibiotics may be required to delay resistance ( e.g. TB )

13. General Principles of Chemotherapy AAAAdminister drug in full dose, at proper interval and by the best route WWWWhen apparent cure achieved, continue antibiotic for about 3 days further to avoid relapse  S S S Skipping doses may decrease effectiveness of antibiotic & increase the incidence of bacterial resistance.

14. General Principles of Chemotherapy(cont.)  Indications for antibiotic combinations: Mixed bacterial infections Mixed bacterial infections Ill patient of unknown etiology Ill patient of unknown etiology Prevent emergence of resistance Prevent emergence of resistance Achieve synergism Achieve synergism Disadvantages of multiple antibiotics Increased risk of sensitivity or toxicity Increased risk of sensitivity or toxicity Increased risk of bacterial resistant Increased risk of bacterial resistant Possibility of antagonism Possibility of antagonism Higher cost Higher cost

15. General principles ( Cont. )  I n some infections bacteriological proof of cure is required.  M easurement of plasma conc. of antibiotics is seldom needed.

16. Indications for antibiotics prophylaxis Surgical prophylaxis bowel surgery, joint replacement, and some gynecological interventions bowel surgery, joint replacement, and some gynecological interventions to prevent postoperative infections. to prevent postoperative infections. Immunosuppressed Patients Very old, very young Very old, very young Diabetics, …… Diabetics, …… Dental extractions Pts with total joint replacements Pts with total joint replacements Pts with cardiac abnormalities Pts with cardiac abnormalities

17. MISUSES OF ANTIBIOTICS  W W W Wrong diagnosis e.g. viral infections  I I I Improper dosage.  T T T Therapy of fever of unknown origin.  P P P Presence of pus or necrotic tissues, or blood at the surgical site  E E E Excessive use of prophylactic antibiotics  L L L Lack of adequate bacteriological information.