1. Eukaryotic Gene Expression The “More Complex” Genome

2. genome characteristics differ dramatically Table 14.1

3. E. coli and yeast, the “eukaryotic E. coli” Table 14.2

4. Table 14.3

5. Table 14.4

6. The Eukaryotic Genome prokaryotic and eukaryotic genomes encode many of the same functions eukaryotes encode additional functions associated with organelles genomes of multicellular eukaryotes encode additional functions each eukaryotic kingdom encodes specialized products –so, eukaryotic genomes are larger –but why so much?

7. Genomes Vary in Size OrganismGenome Size (bp)Genes E. coli4,460,000 (h)4,300 Yeast24,136,0006,200 Nematode97,000,00019,099 Fruit fly180,000,00013,600 Puffer fish365,000,000~30,000 Human6,200,000,00022,000 Arabidopsis119,000,00015,000 (26,000) Rice389,000,00037,544 Lily600,000,000,000??????????????

8. Table 14.5

9. The Eukaryotic Genome Genomics –analyzes and compares entire genomes of different organisms –sequences of many genomes are complete Proteomics –analyzes and compares the functions of the proteins in an cells, tissues, organs, organisms

10. The Eukaryotic Genome repetitive DNA sequences –highly repetitive sequences (10 3 - 10 6 each) tandemly repeated satellites (5-50 bp) –mainly at centromeres minisatellites (12-100 bp) –Variable Number Tandem Repeats microsatellites (1-5 bp x 10-50) –small, scattered clusters untranslated

11. Figure 11.18

12. rRNA genes are tandemly repeated Figure 14.2

13. The Eukaryotic Genome repetitive DNA sequences –moderately repetitive sequences telomeres (~2500 x TTAGGG per chromosome end - human) clustered tRNA, rRNA genes (~280 rRNA coding units on 5 chromosomes - human) transposable elements (transposons)

14. The Eukaryotic Genome transposable elements (transposons) –SINES: transcribed elements ~500 bp long –LINES: elements ~7000 bp long; some are expressed >100,000 copies retrotransposition –retrotransposons: like retroviral genomes –DNA transposons: translocating DNAs

15. Figure 14.3

16. gene expression in eukaryotes Figure 14.1

17. The Eukaryotic Genome Gene expression –protein-coding genes contain non-coding sequences –promoter –terminator –introns interrupt the coding sequence found in exons the primary transcript is processed to produce an mRNA

18. eukaryotic genes contain non-coding regions Figure 14.4

19. Figure 14.5

20. DNA-mRNA hybrids revealed the presence of introns Figure 14.6

21. capping tailing the ends of primary transcripts are processed Figure 14.9

22. The Eukaryotic Genome Gene expression –protein-coding genes primary transcripts are processed to produce mRNAs –the 5’ end is capped with reversed GTP –the 3’ end is given a “poly (A)” tail at the polyadenylation site, AAUAAA –introns are removed during splicing by snRNPs of the spliceosome

23. introns are removed from primary transcripts by spliceosomes Figure 14.10

24. regulation of eukaryotic gene expression may occur at many different points Figure 14.11

25. transcription factors assist RNA polymerase to bind to the promoter Figure 14.12

26. The Eukaryotic Genome expression of eukaryotic genes is highly regulated –three different RNA polymerases transcribe different classes of genes –each RNA polymerase binds to a different class of promoters –RNA polymerases require transcription factors in order to bind to their promoters –transcriptional activators may bind far from the promoter

27. DNA elements are binding sites for proteins of the transcription machinery Figure 14.13

28. DNA looping can bring distant protein factors into contact with the promoter complex Figure 14.13

29. The Eukaryotic Genome expression of eukaryotic genes is highly regulated –eukaryotes do not group genes with related functions together in operons –genes that are coordinately expressed share DNA elements that bind the same transcriptional regulator proteins

30. common response elements enable coordinated expression of independent genes Figure 14.14

31. gene regulators bind to DNA elements common motifs are found among gene regulators Figure 14.15

32. The Eukaryotic Genome many genes are present in single copies –some genes are present in a few similar copies in “gene families” one or more expressed, functional genes non-functional pseudogenes

33. nonfunctional pseudogenes human globin genes are found in two gene families Figure 14.7

34. changes in expression of alternate globin genes Figure 14.8

35. transcription factors remodel chromatin to bind promoters Figure 14.16

36. The Eukaryotic Genome DNA is packaged as chromatin in the nucleus –transcription factors remodel chromatin to bind promoters condensed DNA can “turn off” entire regions of chromosomes

37. The Eukaryotic Genome one gene can encode more than one polypeptide –some primary transcripts undergo alternative splicing

38. alternate splicing: multiple polypeptides from single genes Figure 14.20

39. The Eukaryotic Genome proteins are ultimately removed, degraded and replaced –the proteasome degrades proteins that are tagged for degradation

40. the proteasome recognizes ubiquitin-bound polypeptides Figure 14.22