2. Friday, 11/19/2010 Objective: Chromatography Lab! Get your folder and get ready to start the TIMED BELLRINGER QUESTION.

3. BELL RINGER 1. How do plants get energy? 2. Why do leaves change colors in the fall? 5 MINUTES

4. BELL RINGER 1. How do plants get energy? 2. Why do leaves change colors in the fall? 4 MINUTES

5. BELL RINGER 1. How do plants get energy? 2. Why do leaves change colors in the fall? 3 MINUTES

6. BELL RINGER 1. How do plants get energy? 2. Why do leaves change colors in the fall? 2 MINUTES

7. BELL RINGER 1. How do plants get energy? 2. Why do leaves change colors in the fall? 1 MINUTE

8. TIME’S UP!!!!!

9. Paper Chromatography Lab

10. What is Chromatography? Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components. Separate Analyze Identify Purify Quantify ComponentsMixture

11. Uses for Chromatography Chromatography is used by scientists to: Analyze – examine a mixture, its components, and their relations to one another Identify – determine the identity of a mixture or components based on known components Purify – separate components in order to isolate one of interest for further study Quantify – determine the amount of the a mixture and/or the components present in the sample

12. Illustration of Chromatography MixtureComponents Separation

13. Uses for Chromatography Real-life examples of uses for chromatography: Pharmaceutical Company – determine amount of each chemical found in new product Hospital – detect blood or alcohol levels in a patient’s blood stream Law Enforcement – to compare a sample found at a crime scene to samples from suspects Environmental Agency – determine the level of pollutants in the water supply Manufacturing Plant – to purify a chemical needed to make a product

14. Overview of the Experiment Purpose: To explain the relationship between pigments and photosynthesis. To predict which pigment colors would be best for photosynthesis.

15. 100 mL beaker Isopropyl Alcohol (10 mL) Chromatography paper strip Spinach leaf solution – Red leaf lettuce solution Disposable Pipette Pencil Ruler Scissors Tape Materials List

16. Preparing the Chromatography Strips 2. Starting at the line, taper the bottom of the paper with scissors. 3. Place 1-2 drops of the spinach leaf solution on the starting line and allow to dry 1. Draw a line 1.5 cm above the bottom edge of the strip with the pencil

17. Developing the Chromatograms Place the strip in the beaker Make sure the solution does not come above the drop on your start line Fold the top of the strip over your beaker and tape to keep in place Let strips develop for approximately 15 minutes Remove the strips and let them dry

18. Use the table on your lab handout to analyze your chromatography strip Answer the analysis questions in your lab notebook RESULTS

19. Debrief/Wrap Up Knowledge of Inquiry *Scientific investigation begin with a problem but don’t necessarily test a hypothesis *There is no single scientific method *Inquiry procedures are guided by a question *All scientists performing the same procedures may not get the same results *Inquiry procedures can influence results *Conclusions must be consistent with the data *Scientific data is not the same as scientific evidence *Explanations are developed from the data and what is known NOS * Tentativeness *Creativity *Observation vs. Inference *Subjectivity *Theory and law *Socially/culturally embedded *Empircally based Suggestions/Comments: