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Thin Layer Chromatography bsapp.com. Preparing a Sample Space Cut a sheet of paper so it may hang free within the intended container Mark a sample line.

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Presentation on theme: "Thin Layer Chromatography bsapp.com. Preparing a Sample Space Cut a sheet of paper so it may hang free within the intended container Mark a sample line."— Presentation transcript:

1 Thin Layer Chromatography bsapp.com

2 Preparing a Sample Space Cut a sheet of paper so it may hang free within the intended container Mark a sample line ½ inch to an inch from the bottom bsapp.com

3 Place your samples on the sheets bsapp.com

4 Starting the Sample Place the sheet in a container with a solvent The bottom of the paper should be submerged into the solvent, but not above the samples bsapp.com

5 Allow your samples to run at least ½ way up the sample space bsapp.com

6 bsapp.com

7 Allow your samples to drybsapp.com

8 Matching Samples Note component colors Calculate Rf values bsapp.com

9 Calculating Rf Values Rf value is a ratio of distance a component sample moves to the distance the solvent moves This number is usually expressed as a decimal bsapp.com

10 Calculating Rf Values  Starting point  8 cm  4 cm  Solvent finish 10 cm Rf = 4/10 = 0.4 Rf = 8/10 = 0.8 bsapp.com


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