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Published byJared Alexander Modified over 9 years ago
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Thin Layer Chromatography bsapp.com
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Preparing a Sample Space Cut a sheet of paper so it may hang free within the intended container Mark a sample line ½ inch to an inch from the bottom bsapp.com
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Place your samples on the sheets bsapp.com
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Starting the Sample Place the sheet in a container with a solvent The bottom of the paper should be submerged into the solvent, but not above the samples bsapp.com
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Allow your samples to run at least ½ way up the sample space bsapp.com
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Allow your samples to drybsapp.com
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Matching Samples Note component colors Calculate Rf values bsapp.com
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Calculating Rf Values Rf value is a ratio of distance a component sample moves to the distance the solvent moves This number is usually expressed as a decimal bsapp.com
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Calculating Rf Values Starting point 8 cm 4 cm Solvent finish 10 cm Rf = 4/10 = 0.4 Rf = 8/10 = 0.8 bsapp.com
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