2. Program will begin shortly… “Inner Life of the Cell”, Harvard University © 2008 Up Next … Making Molecular Movies with Up Next … Making Molecular Movies with Insert video: InnerLifeTrimmed.m4v

3. Making Molecular Movies with Making Molecular Movies with Your Name, Institution

4. The Team Peter Kner Microscopist Jessica Winter Chemical Engineer Chemical Engineer Ge Yang Bioengineer Bioengineer Beth Brainerd Biologist University of Georgia

5. qstorm.org Visit us online!

6. qstorm.org Visit us online! Time is ticking. Can these folks make a major breakthrough in biological imaging?

7. 48 hours in 40 seconds Credit: www.broad.com; The Ark Hotel Insert video: TimeLapseConstruction.m4v

8. 10 microns Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center

9. 10 microns Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center Insert video: CellDivision-Short.m4v

10. 10 microns Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center Insert video: CellDivision-Short.m4v

11. 10 microns Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center Insert video: CellDivision-Short.m4v

12. 10 microns Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center Insert video: CellDivision-Short.m4v

13. The Team Peter Kner Microscopist Jessica Winter Chemical Engineer Chemical Engineer Ge Yang Bioengineer Bioengineer Beth Brainerd Biologist University of Georgia

14. Beth Brainerd Biologist (Muscle Cells)

15. Zebrafish Muscle Cells Credit: Mezzotint Insert video: loop-and-slow-motion-female-sp_prores.mov

16. Ge Yang Bioengineer (Nerve Cells)

17. Credit: “Inside the Brain”, NIH National Institute on Aging Nerve Cells

18. Insert video: InnerLifeTrimmed.m4v “Inner Life of the Cell”, Harvard University © 2008

19. ≈ 100 nanometers

20. “Inner Life of the Cell”, Harvard University © 2008 ≈ 100 nanometers

21. Motor Protein

22. Much smaller than a wavelength of visible light 30 nm

23. Point light source 30 nm

24. Optical Limit: 200 nanometers 200 nm

25. Overlapping Blurs 200 nm

26. The Team Peter Kner Microscopist Jessica Winter Chemical Engineer Chemical Engineer Ge Yang Bioengineer Bioengineer Beth Brainerd Biologist University of Georgia

27. Peter Kner Microscopist

28. STORM Credit: MovingImages Insert video: massive-electrical-storm-light_prores.m4v Insert sound: Perfect Thunder Storm-Sound.mp3 Insert video: massive-electrical-storm-light_prores.m4v Insert sound: Perfect Thunder Storm-Sound.mp3

29. STORM IMAGING

30. STORM ST ochastic O ptical R econstruction M icroscopy

31. STORM Xiaowei Zhuang 2006 Credit: FredField.com

32. Standard optical microscope image Credit: Bates et al. Science 317, 1749-1753 (2007) 3 μm

33. Loss of resolution when zoomed into the nanoscale Credit: Bates et al. Science 317, 1749-1753 (2007)

34. Standard Microscope Image Diffraction-Limited STORM Image Super Resolution

37. + + + + + + +

38. + + + + + + +

40. + + + + + + +

41. Localization + + + + + + +

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44. + + + + + + +

45. Insert video: Particle Detection.m4v

46. Greetings from Insert video: Reconstructed Image.m4v

47. Credit: Ricardo Henriques, Institut Pasteur STORM Imaging at Work Stochastic (Random) Blinking Reconstruction Insert video: Particle Detection.m4v (on left side) and Reconstructed Image.m4v (on right side)

48. Credit: Ricardo Henriques, Institut Pasteur STORM Imaging at Work Particle Detection and Localization Reconstruction

49. STORM Imaging of Cell Structures Credit: Rust et al. Nature Methods 3, 793-795 (2006) and Bates et al. Science 317, 1749-1753 (2007) Insert video: Zhuang_storm_short_slow.avi

50. Credit: Bates et al. Science 317, 1749-1753 (2007) STORM – Depends on bright light sources

51. Credit: Bates et al. Science 317, 1749-1753 (2007) Less than 30 nm ! STORM – Depends on bright light sources

52. Credit: Bates et al. Science 317, 1749-1753 (2007) STORM – How to Get Even Greater Resolution? Less than 30 nm ! We need more photons!

53. The Team Peter Kner Microscopist Jessica Winter Chemical Engineer Chemical Engineer Ge Yang Bioengineer Bioengineer Beth Brainerd Biologist University of Georgia

54. Jessica Winter Chemical Engineer

55. Quantum Dots in Solution Credit: Felice Frankel Nano Crystals of Different Sizes

56. Jessica Winter Chemical Engineer

57. Credit: Ricardo Henriques Insert video: Particle Detection.m4v

58. 10 nm

60. Linker

66. SuperHeroe s

67. Insert video: vim-NEiA_Fall 2010_MoS_QSTORM_Final.mov Or download or play from: http://vimeo.com/18462954http://vimeo.com/18462954 Insert video: vim-NEiA_Fall 2010_MoS_QSTORM_Final.mov Or download or play from: http://vimeo.com/18462954http://vimeo.com/18462954

68. qstorm.org Visit us online!

69. An MOS Strategic Projects Production by C.L. Alpert and K. Thate with assistance from J. Antill and the QSTORM Research Collaborative Production of this project was supported by the National Science Foundation, MCB-1052733. Dissemination of this project was supported by the NISE Network through the National Science Foundation under Award No. 0940143. Any opinions, findings, and conclusions or recommendations are those of the author(s) and do not necessarily reflect the views of the Foundation. Published under a Creative Commons Attribution-Noncommercial- ShareAlike license: http://creativecommons.org/licenses/by-nc-sa/3.0/ushttp://creativecommons.org/licenses/by-nc-sa/3.0/us Join us at www.QSTORM.org Making Molecular Movies with