1. Figure S1 Approximate positions of primers listed in Supplementary Table S1 for F3H (a), Tdic101 (b) and dTdic102 (c). Black arrow heads show primers and their orientation. Coding regions (shaded boxes) in F3'H, the transposase (black bar) in Tdic101 and the TIRs (terminal inverted repeats, open triangles) are indicated. a bc 9 15 19 1618 13 7 4 1 2 (G) HindIII 3 5 6 8 10 14 Tdic101 insertion site 17 20 21 23 HindIII 11 122224 2526 27 28 29 30 31 32 33 F3H Tdic101dTdic102

2. 0.8 1.1 23.1 6.6 2.3 9.4 2.0 (kbp) MPu MPi 1 2 3 4 5 6 7 8 Fig. S2 Analysis of the vacated site of the 4.3 kb (Tdic101) insertion in the F3H gene. PCR products are shown that are obtained with gene- specific primers on DNAs isolated from six different reverted, purple regions emerging in MPi flowers (1-6) or from pink regions in partly reverted variegated MPi flowers (7-8). Analysis by PCR of DNA isolated from MPu and MPi is shown and the migration of molecular weight markers is indicated on the left.

3. Fig. S3 Schematic structure of the putative Tdic101 transposase (TPase). Black boxes represent the six conserved hAT-transposase motifs reported by Rubin et al. (2001). The number of amino acids (aa) separating these motifs in the Tdic101 TPase are indicated. A; CKFNLTADVWDSD; VLDLLLWWKGQ B; HVRCACHVLNLCVE; LPVMSAMAQDFLSIQVSSVASERAFS C; TRWNSMYIMLF; SKRVLDEKRTSLRSDTLEMCVCYKDW ABCDEF 88 aa50 aa226 aa3 aa1 aa320 aa28 aa TPase (814 aa)