1. Ta-3 MKTFPVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-41 MKTFPVFALPAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-43 MKTFPVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-4 MKTFPVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-42 MKTFPVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-45 MKKNLVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQRQPFPQQQQPPFSQQQPSFSQRQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-5 MKKNLVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-44 MKKNLVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPSFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-27 MKTFLVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-28 MKTFLVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-19 MKTFLIFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-21 MKTFLIFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-17 MKTFLIFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-26 MKTFLVFALLAIVATSVIAQVETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-31 MRTFLVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSSSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-33 MRTFLVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-37 MRTFLVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-38 MRTFLVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQLPFSQQQQPALPQQSPFL 101 Ta-24 MKTFLVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-32 MRTFLVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-29 MKTFLVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-30 MKTFLVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-35 MRTFLVFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQPPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 Ta-22 MKTFLIFALLAIVATSVIAQMETSCIPGLERPWQQQPLPPQQTLFPQQQPFPQQQQPPFSQQQPSFSQQQLPFSQQQPILPQQPPFSQQQQPALPQQSPFL 101 QQQQLVLPPQQQHQQLLQQQIPIVQPSVLQQLNPCKVFLQQKCSPVAMPQRLARSQMWQQSSCHVMQQQCCQQLPQIPEQSRYEAIRAITYSIILQEQQQG 202 QQQQLVLPPQQQHQQLLQQQIPIVQPSVLQQLNPCKVFLQQKCSPVAMPQRLARSQMWQQSSCHVMQQQCCQQLPQIPEQSRYEAIRAITYSTILQEQQQG 202 QQQQLVLPPQQQHQQLLQQQIPIVQPSVLQQLNPCKVFLQQKCSPVAMPQRLARSQMWQQSSCHVMQQQCCQQLPQIPEQSRYEAIRAITYPIILQEQQQG 202 QQQQLVLPPQQQHQQLLQQQIPIVQPSVLQQLNPCKVFLQQKCSPVAMPQRLARSQMWQQSSCHVMQQQCCQQLPQIPEQSRYEAIRAITYSIILQEQQQG 202 QQQQLVLPPQQQHQQLLQQQIPIVQPSVLQQLNSCKVFLQQKCSPVAMPQRLARSQMWQQSSCHVMQQQCCQQLPQIPEQPRYEAIRAITYSIILQEQQQG 202 QQQQLVLPPQQQHQQLLQQQIPIVQPSVLQQLNPCKVFLQQKCSPVAMPQRLARSQMWQQSSCHVMQQQCCQQLPQIPEQSRYEAIRAITYSIILQEQQQG 202 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVGAY 303 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVGA 302 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVGSY 303 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVAAY 303 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVSAY 303 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQRPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVSAY 303 FVQPQQQQPQQSGQGVSQSQQQSQQQPGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVAAY 303 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQVAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVGAY 303 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLRPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVSAY 303 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVAAY 303 FVQPQQQRPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVSAY 303 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVAAY 303 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVGA 302 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVGAY 303 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVGA 302 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCLFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVSAY 303 FVQPQQQQPQQSGQGASQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVAAY 303 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVGVY 303 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQRPQQQQVLQGTFLQPHQIAHLEVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVGPY 303 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLKVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVGSY 303 FVQPQQQQPQQSGQGVSQSQQQSQQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLGVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVGGY 303 FVQPQQQQPQQSGQGVSQSQQQSRQQLGQCSFQQPQQQLGQQPQQQQVLQGTFLQPHQIAHLGVMTSIALRTLPTMCSVNVPLYSSTTSVPFSVGTGVGPY 303 a Signal peptide N-terminal Repetitive domain C-terminal domain( )

2. Ta-18 MKTFLIFALLATAATSAIAQMETSRVPGLEKPWQQQPLSPQQQPPCSQQQQPLPQQQQPIIILQQPPFSQQQQPVLPQQQQPVIILQQ 88 Ta-20 MKTFLIFALLAIAATSAIAQMETSRVPGLEKPWQQQPLSPQQQPPCSQQQQPLPQQQQPIIILQQPPFSRQQQPVLPQQQQPVIILQQ 88 Ta-16 MKTFLIFALLAIAATSAIAQMETSRVPGLEKPWQQQPLSPQQQPPCSQQQQPLPQQQQPIIILQQPPFSQQQQPVLPQQQQPVIILQQ 88 Ta-25 MKTFLVFALLAIAATSAIAQMETSRVPGLEKPWQQQPLSPQQQPPCSQQQQPLPQQQQPIIILQQPPFSQQQQPVLPQQQQPVIILQQ 88 Ta-36 MRTFLVFALLAIAATSAIAQMETSRVPGLEKPWQQQPLSPQQQPPCSQQQQPLPQQQQPIIILQQPPFSQQQQPVLPQQQQPVIILQQ 88 Ta-7 MKTFPVFALLAIAATSAIAQMETSRVPGLEKPWQQQPLQPQQQPPCSQQQQPLPQQQQPIIILQQPPFSQQQQPVLPQQQQPVIILQQ 88 Ta-10 MKTFLIFALLAIAATSAIAQMETSRVPGLEKPWQQQPLPPPQQPPCSQQQQPLPQQQQPIIILQQPPFSQQQQPVLPQQRQPVIILQQ 88 Ta-11 MKTFLVFALLAIAATSAITQMETSRVPGLEKPWQQQPLPPPQQPPCSQQQQPLPQQQQPIIILQQPPFSQQQQPVLPQQQQPVIILQQ 88 Ta-13 MRTFLVFALLAIAATSAIAQMETSRVPGLEKPWQQQPLPPPQQPPCSQQQQPLPQQQQPIIILQQPPFSQQQQPVLPQQQQPVIILQQ 88 PPFLEQQQPVLPQQPSFSQQQQQQQQQPPFLEQQQPVLPQQPSFSQQQQQQQQPFPQQQQPSSQQQPFPQQHQHLLQQQIPVVQPSVL 176 PPFLEQQQPVLPQQPSFSQQQQQQQQQPPFLEQQQPVLPQQPSFSQQQQQQQQPFPQQQQPSSQQQPFSQQHQHLLQQQIPVVQPSVL 176 PPFLEQQQPVLPQQPSFSQQQQQQQQQPPFLEQQQPVLPQQPSFSQQQQQQQQPFPQQQQPSSQQQPFPQQHQHLLQQQIPVVQPSVL 176 PPFLEQQQPVLPQQPSFSQQQQQQ..QPPFLEQQQPVLPQQPSFSQQ......PFPQQQQPSSQQQPFPQQHQHLLRQQIPVVQPSVL 168 PPFLEQQQPVLPQQPSFSQQQQQQ..QQPFLEQQQPVLPQQPSFSQQQQQQQ.PFPQQQQPSSQQQPFPQQHQHPLQQQIPVVQPSVL 173 PPFLEQQQPVLPQQPPFSQQQQQQ..QQPFLEQQQPVLPQQPSFSQQQQQQQ.PFPQQQQPSSQQQPFPQQHQHPLQQQIPVVQPSVL 173 PPFLEQQQPVLPRQPSFSQQQQQQ..QQPFLEQQQPVLPQQPSFSQQQQQQQ.PFPQQQQPSSQQQPFPQQHQHPLQQQIPVVQPSVL 173 QQLHPCKVFLQQQCSHVAMSQRLARSQMWQQSSCHVMQQQCCQQLPQIPEQSRYEAIRTIVYSIILQEQQQGFVQPQQQQPQQLGQGV 264 QQLHPCKVFLQQQCSHVAMSQRLARSQMWQQSSCHVMQQQCCQQLPQIPEQSRYEAIRTIVYSIILQEQRQGFVQPQQQQPQQLGQGV 264 QQLHPCKVFLQQQCSHVAMSQRLARSQMWQQSSCHVMQQQCCQQLPQIPEQSRYEAIRTIVYSIILQEQQQGFVQPQQQQPQQLGQGV 264 QQLHPCKVFLQQQCSHVAMSQRLARSQMWQQSSCHVMQQQCCQQLPQIPEQSRYEAVRTIVYSIILQEQQQGFVQPQQQQPQQLGQGV 264 QQLHPCKVFLQQQCSHVAMSQRLARSQMWQQSSCHVMQQQCCQQLPQIPEQSRYEAIRTIVYSIILQEQQQGFVQPQQQQPQQLGQGV 264 QQLHPCKVFLQQQCSHVAMSQRLARSQMWQQSSCHVMQQQCCQQLPQIPEQSRYEAIRAIVYSIILQEQQQGFVQPQQQQPQQLGQGV 256 QQLHPCKVFLQQQCSHVAMSQRLARSQMWQQSSCHVMQQQCCQQLPQIPEQSRYEAIRAIVYSIILQEQQQGFVQPQQQQPQQLGQGV 261 QQLHPCKVFLQQQCSLVAMSQRLARSQMWQQSSCHVMQQQCCQQLPQIPEQSRYEAIRAIVYSIILQEQQQGFVQPQQQQPQQLGQGV 261 QQLHPCKVFLQQQCSHVAMSQRLARSQMWQQSSCHVMQQQCCQQLPQIPEQSRYEAIRAIVYSIILQEQQQGFVQPQQQQPQQLGQGV 261 SQPQQQSQQQQLGQCSFQQPQQQQLGQQPQQQQIPQGTFLQPHQISQLEVMTSIALRTLPTICGVNVPLYSSTTSVPFGIGTGVGGY 351 SQPQQQSQQQQLGQCSFQQPQQQQLGQQPQQQQIPQGTFLQPHQISQLEVMTSIALRTLPTICGVNVPLYSSTTSVPFGIGTGVSAY 351 SQPQQQSQQQQLGQCSFQQPQQQQLGQQPQQQQIPQGTFLQPHQISQLEVMTSIALRTLPTICGVNVPLYSSTTSVPFGIGTGVGVY 351 SQPQQQSQQQQLGQCSFQQPQQQQLGQQPQQQQIPQGTFLQPHQISQLEVMTSIALRTLPTICGVNVPLYSSTTSVPFGIGTGVGGY 351 SQPQQQSQQQQLGQCSFQQPQQQQLGQQPQQQQIPQGTFLQPHQISQLEVMTSIALRTLPTICGVNVPLYSSTTSVPFGIGTGVGVY 343 SQPQQQSQQQQLGQCSFQQPQQQQLGQQPQQQQIPQGTFLQPHQISQLEVMTSIALRTLPTICGVNVPLYSSTTSVPFGIGTGVAAY 348 SQPQQQSQQQQLGQCSFQQPQQQQLGQQPQQQQIPQGTFLQPHQISQLEVMTSIALRTLPTICGVNVPLYSSTTSVPFGIGTGVSAY 348 b Signal peptide N-terminal Repetitive domain C-terminal domain( ) Supplementary Fig.1 Multiple sequence alignments of the deduced amino acid sequences of 33 genes in Taeniatherum. The 24 and 9 genes with N-terminal sequence METSCIPG- and METSRVP are shown in a and b, respectively. Cysteine residues (c) are underlined, hydrophobic clusters are boxed