1. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Human Anatomy & Physiology, Sixth Edition Elaine N. Marieb PowerPoint ® Lecture Slides prepared by Vince Austin, University of Kentucky 8 Muscles and Muscle Tissue Part A

2. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Muscle Overview  Muscle tissue makes up nearly half the body mass.  The most distinguishing functional characteristic of muscles is their ability to transform chemical energy ATP into directed mechanical energy  The three types of muscle tissue are skeletal, cardiac, and smooth  These types differ in structure, location, function, and means of activation

3. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Muscle Similarities  Skeletal and smooth muscle cells are elongated and are called muscle fibers  Muscle contraction depends on two kinds of myofilaments – actin and myosin  Muscle terminology is similar  Sarcolemma – muscle plasma membrane  Sarcoplasm – cytoplasm of a muscle cell  Prefixes – myo, mys, and sarco all refer to muscle

4. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Skeletal Muscle Tissue  Packaged in skeletal muscles that attach to and cover the bony skeleton  Has obvious stripes called striations  Is controlled voluntarily (i.e., by conscious control)  Contracts rapidly but tires easily  Is responsible for overall body motility  Is extremely adaptable and can exert forces ranging from a fraction of an ounce to over 70 pounds  Key words: skeletal, striated and voluntary

5. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Cardiac Muscle Tissue  Occurs only in the heart (“blood pump”)  Is striated like skeletal muscle but is not voluntary  Contracts at a fairly steady rate set by the heart’s pacemaker  Neural controls allow the heart to respond to changes in bodily needs  Key words: cardiac, striated and involuntary

6. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Smooth Muscle Tissue  Found in the walls of hollow visceral organs, such as the stomach, urinary bladder, and respiratory passages  Forces food and other substances through internal body channels  It is not striated and is involuntary  Key words: visceral, non striated and involuntary

7. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Functional Characteristics of Muscle Tissue  Excitability, or irritability – the ability to receive and respond to stimuli  Contractility – the ability to shorten forcibly  Extensibility – the ability to be stretched or extended  Elasticity – the ability to recoil and resume the original resting length

8. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Muscle Function  Skeletal muscles are responsible for all locomotion  Cardiac muscle is responsible for coursing the blood through the body  Smooth muscle helps maintain blood pressure, and squeezes or propels substances (i.e., food, feces) through organs  Muscles also maintain posture, stabilize joints, and generate heat

9. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Skeletal Muscle Gross Anatomy Tissues: –Blood vessels –Nerves – branches to each fiber –Connective Tissue Endomysium –wraps each fiber Perimysium –wraps fibers into fascicles Epimysium –wraps fascicles into a muscle All are continuous with each other and the tendons.

10. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Skeletal Muscle ( Table 9.1) Figure 9.2 (a)

11. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Skeletal Muscle: Nerve and Blood Supply  Each muscle is served by one nerve, an artery, and one or more veins  Each skeletal muscle fiber is supplied with a nerve ending that controls contraction  Contracting fibers require continuous delivery of oxygen and nutrients via arteries  Wastes must be removed via veins

12. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Structural Organization of Skeletal Muscle Muscle (Organ) Fascicles (Bundles of fibers) Fiber (Cell) Myofibrils ( densely packed contractile elements) Myofilaments (contractile proteins) –Actin (thin filaments) –Myosin (thick filaments)

13. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Myofibrils Figure 9.3 (b)

14. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Sarcomeres  The smallest contractile unit of a muscle  The region of a myofibril between two successive Z discs  Composed of myofilaments made up of contractile proteins  Myofilaments are of two types – thick and thin

15. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Sarcomeres Figure 9.3 (c)

16. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Myofilaments: Banding Pattern  Thick filaments (composed by myosin) – extend the entire length of an A band  Thin filaments (composed by actin) – extend across the I band and partway into the A band  Z-disc – coin-shaped sheet of proteins (connectins) that anchors the thin filaments and connects myofibrils to one another

17. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Myofilaments: Banding Pattern Figure 9.3 (c, d)

18. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Ultrastructure of Myofilaments: Thick Filaments  Thick filaments are composed of the protein myosin  Each myosin molecule has a rodlike tail and two globular heads  Tails – two interwoven, heavy polypeptide chains  Heads – two smaller, light polypeptide chains called cross bridges

19. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Ultrastructure of Myofilaments: Thick Filaments Figure 9.4 (a)(b)

20. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Ultrastructure of Myofilaments: Thin Filaments  Thin filaments are chiefly composed of the protein actin  Each actin molecule is a helical polymer of globular subunits called G actin  The subunits contain the active sites to which myosin heads attach during contraction  Tropomyosin and troponin are regulatory subunits bound to actin

21. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Ultrastructure of Myofilaments: Thin Filaments Figure 9.4 (c)

22. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Arrangement of the Filaments in a Sarcomere  Longitudinal section within one sarcomere Figure 9.4 (d)

23. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Sliding Filament Model of Contraction  Thin filaments slide past the thick ones so that the actin and myosin filaments overlap to a greater degree  In the relaxed state, thin and thick filaments overlap only slightly  Upon stimulation, myosin heads bind to actin and sliding begins

24. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Sliding Filament Model of Contraction  Each myosin head binds and detaches several times during contraction, acting like a ratchet to generate tension and propel the thin filaments to the center of the sarcomere  As this event occurs throughout the sarcomeres, the muscle shortens

25. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Skeletal Muscle Contraction  In order to contract, a skeletal muscle must:  Be stimulated by a nerve ending  Propagate an electrical current, or action potential, along its sarcolemma  Have a rise in intracellular Ca 2+ levels, the final trigger for contraction

26. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Brain  Spinal Cord  Motor Neuron  Muscle Motor = movement Neuron = nerve cell

27. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Motor Neuron  Muscle Fiber Motor neuron releases neurotransmitter called acetylcholine (ACh). ACh causes muscle fiber to produce an electrical signal. Electrical signal causes actin & myosin to move, and this causes muscle to move.

28. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Destruction of Acetylcholine  ACh bound to ACh receptors is quickly destroyed by the enzyme acetylcholinesterase  This destruction prevents continued muscle fiber contraction in the absence of additional stimuli

29. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings A Myofibril at Rest

30. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings A Myofibril at Rest At rest” = fiber has not received message to move Actin (thin) attaches to Z-line. Myosin (thick) does not attach to actin or the Z-line. Myosin heads are bent back (cocked).

31. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Contraction Let’s compare length of sarcomeres: Relaxed: Contracted:

32. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Contraction in the Myofibril After contraction, muscle relaxes (back to original length). – Myosin heads pick up ATP & break it apart. – This cocks the head, detaching it from actin. Now ready to contract again. Head is Cocked ATP

33. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Sequential Events of Contraction  Cross bridge formation – myosin cross bridge attaches to actin filament  Working (power) stroke – myosin head pivots and pulls actin filament toward M line  Cross bridge detachment – ATP attaches to myosin head and the cross bridge detaches  “Cocking” of the myosin head – energy from hydrolysis of ATP cocks the myosin head into the high-energy state

34. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Contraction

35. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings

36. Contraction of Skeletal Muscle (Organ Level)  Contraction of muscle fibers (cells) and muscles (organs) is similar  The two types of muscle contractions are:  Isometric contraction – increasing muscle tension (muscle does not shorten during contraction)  Isotonic contraction – decreasing muscle length (muscle shortens during contraction)

37. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Types of muscular contraction Isometric –Muscular contraction where the tension developed occurs with no change in length –Otherwise known as static contraction or position. –Improves muscular strength at fixed joint angle –Does not develop aerobic fitness –Can be done anywhere  Examples; rugby scrum, tug of war.

38. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Types of muscle contraction Isotonic Muscles contact at speed controlled by the performer Motor unit recruitment is at the speed required for the specific sports activity. Develops aerobic and anaerobic fitness Most physical activities are isotonic Can occur in two ways: concentric and eccentric.

39. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Contraction Concentric contraction – Muscle shortens under tension – Insertion moves towards origin – Occurs in agonist muscle – e.g. Chin-ups – use of bicep brachii in upward phase

40. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Contraction Eccentric contraction Muscle lengthens under tension Insertion moves away from origin Occurs in antagonist muscle Only occurs if the antagonist is acting as a brake to help control the joint movement E.g. Chin-ups – use of biceps in downward phase.