1. Animal Physiology and Development Insects – 4 JCS Lecture Outcome: By the end of this lecture student should have: a)Become aware of the role of the imaginal discs in imaginal development. b)Understood the evidence for their determined state as a result of transplantation experiments. c)Appreciated how the determination becomes more specific as larval development progresses and the discs grow and how this can be determined using mitotic recombination.. d)Understood the concept and evidence of compartments. e)Followed the development of one imaginal disc – the wing disc. JCS 6/11/02

2. Location of imaginal discs within the larva Transplant experiments – discs into meta- morphosing larvae give rise to specific adult parts in the abdomen of the hosts

3. Transplantation of cut pieces of discs into meta- morphosing larval hosts shows that in mature disc each part is determined. Note: that the discs are folded and the distal parts are central Wing disc Leg disc The study of disc development is very much the investigation of how this occurs. For developmental biologists the nice thing about imaginal discs is that they are discrete developmental fields and easily manipulated.

4. Relationship between disc structure/ determination and final adult structure of the wing and the leg.

5. Determination state is stable – persists on extended culture of discs in female abdomens…..but change of disc from one disc to another type can occur – Transdetermination.

6. Marking a single cell and its mitotic descendants by creating a genetic change (mitotic recombination) using X-rays The clone is visible because the cell from which the clones originates is made homozygous for recessive mutants which colour the cuticle or facets of the eye or the affect shape/number of bristles and hairs.

7. How mitotic clones are poroduced with X-rays Minute + /Minute - cells grow slowly compared to wild type Homozygous Minute - cells die. Wild-type cells thus grow to become very large clones in a Minute + /Minute - organism

8. Disc growth from clonal analysis Very early induction of Minute clones

9. Large clones can show restrictions – i.e. cells and their descendants are restricted to compartments by compartment boundaries Minute + clone Note how the large ‘Minute’ clones readily define the compartment border

10. engrailed mutation affects the wing (see below). Gene is expressed in every segment of the early embryo and then is maintained throughout disc development. engrailed is a segment polarity gene. It

11. Signalling to derive compartments and specify the determination of the wing.

12. Leg disc - gene expression patterns and eversion Wing disc – dorso-ventral compartments and eversion

14. From oogenesis to the imago