1. PowerPoint ® Lecture Slide Presentation by Patty Bostwick-Taylor, Florence-Darlington Technical College Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings PART B 3 Cells and Tissues

2. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Cell Physiology: Membrane Transport  Membrane transport—movement of substances into and out of the cell  Two basic methods of transport  Passive transport  No energy is required  Active transport  Cell must provide metabolic energy (ATP)

3. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Solutions and Transport  Solution—homogeneous mixture of two or more components  Solvent—dissolving medium; typically water in the body  Solutes—components in smaller quantities within a solution  Intracellular fluid—nucleoplasm and cytosol  Interstitial fluid—fluid on the exterior of the cell

4. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Selective Permeability  The plasma membrane allows some materials to pass while excluding others  This permeability influences movement both into and out of the cell

5. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Passive Transport Processes  Diffusion  Particles tend to distribute themselves evenly within a solution  Movement is from high concentration to low concentration, or down a concentration gradient Figure 3.9

6. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Passive Transport Processes  Types of diffusion  Simple diffusion  An unassisted process  Solutes are lipid-soluble materials or small enough to pass through membrane pores

7. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Passive Transport Processes Figure 3.10a

8. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Passive Transport Processes  Types of diffusion (continued)  Osmosis—simple diffusion of water  Highly polar water molecules easily cross the plasma membrane through aquaporins

9. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Passive Transport Processes Figure 3.10d

10. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Passive Transport Processes  Facilitated diffusion  Substances require a protein carrier for passive transport  Transports lipid-insoluble and large substances

11. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Passive Transport Processes Figure 3.10b–c

12. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Passive Transport Processes  Filtration  Water and solutes are forced through a membrane by fluid, or hydrostatic pressure  A pressure gradient must exist  Solute-containing fluid is pushed from a high-pressure area to a lower pressure area

13. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes  Substances are transported that are unable to pass by diffusion  Substances may be too large  Substances may not be able to dissolve in the fat core of the membrane  Substances may have to move against a concentration gradient  ATP is used for transport

14. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes  Two common forms of active transport  Active transport (solute pumping)  Vesicular transport  Exocytosis  Endocytosis  Phagocytosis  Pinocytosis

15. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes  Active transport (solute pumping)  Amino acids, some sugars, and ions are transported by protein carriers called solute pumps  ATP energizes protein carriers  In most cases, substances are moved against concentration gradients

16. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Figure 3.11 Extracellular fluid Cytoplasm Loss of phosphate restores the original conformation of the pump protein. K + is released to the cytoplasm and Na + sites are ready to bind Na + again; the cycle repeats. Binding of cytoplasmic Na + to the pump protein stimulates phosphorylation by ATP, which causes the pump protein to change its shape. The shape change expels Na + to the outside. Extracellular K + binds, causing release of the phosphate group. ADP Na + K+K+ K+K+ K+K+ K+K+ P P P ATP

17. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Figure 3.11, step 1 Extracellular fluid Cytoplasm Binding of cytoplasmic Na + to the pump protein stimulates phosphorylation by ATP, which causes the pump protein to change its shape. ADP Na + P ATP

18. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Figure 3.11, step 2 Extracellular fluid Cytoplasm Binding of cytoplasmic Na + to the pump protein stimulates phosphorylation by ATP, which causes the pump protein to change its shape. The shape change expels Na + to the outside. Extracellular K + binds, causing release of the phosphate group. ADP Na + K+K+ K+K+ P P P ATP

19. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Figure 3.11, step 3 Extracellular fluid Cytoplasm Loss of phosphate restores the original conformation of the pump protein. K + is released to the cytoplasm and Na + sites are ready to bind Na + again; the cycle repeats. Binding of cytoplasmic Na + to the pump protein stimulates phosphorylation by ATP, which causes the pump protein to change its shape. The shape change expels Na + to the outside. Extracellular K + binds, causing release of the phosphate group. ADP Na + K+K+ K+K+ K+K+ K+K+ P P P ATP

20. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes  Vesicular transport  Exocytosis  Moves materials out of the cell  Material is carried in a membranous vesicle  Vesicle migrates to plasma membrane  Vesicle combines with plasma membrane  Material is emptied to the outside

21. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes: Exocytosis Figure 3.12a

22. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes: Exocytosis Figure 3.12b

23. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes  Vesicular transport (continued)  Endocytosis  Extracellular substances are engulfed by being enclosed in a membranous vescicle  Types of endocytosis  Phagocytosis—“cell eating”  Pinocytosis—“cell drinking”

24. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes: Endocytosis Figure 3.13a Recycling of membrane and receptors (if present) to plasma membrane Cytoplasm Extracellular fluid Plasma membrane Detachment of vesicle Vesicle containing ingested material Vesicle Vesicle fusing with lysosome for digestion Release of contents to cytoplasm Lysosome Transport to plasma membrane and exocytosis of vesicle contents Plasma membrane Ingested substance Pit (a)

25. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes: Endocytosis Figure 3.13a, step 1 Cytoplasm Extracellular fluid Plasma membrane Ingested substance Pit (a)

26. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes: Endocytosis Figure 3.13a, step 2 Cytoplasm Extracellular fluid Plasma membrane Detachment of vesicle Vesicle containing ingested material Plasma membrane Ingested substance Pit (a)

27. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes: Endocytosis Figure 3.13a, step 3 Cytoplasm Extracellular fluid Plasma membrane Detachment of vesicle Vesicle containing ingested material Vesicle Vesicle fusing with lysosome for digestion Lysosome Plasma membrane Ingested substance Pit (a)

28. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes: Endocytosis Figure 3.13a, step 4 Cytoplasm Extracellular fluid Plasma membrane Detachment of vesicle Vesicle containing ingested material Vesicle Vesicle fusing with lysosome for digestion Release of contents to cytoplasm Lysosome Plasma membrane Ingested substance Pit (a)

29. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes: Endocytosis Figure 3.13a, step 5 Cytoplasm Extracellular fluid Plasma membrane Detachment of vesicle Vesicle containing ingested material Vesicle Vesicle fusing with lysosome for digestion Release of contents to cytoplasm Lysosome Transport to plasma membrane and exocytosis of vesicle contents Plasma membrane Ingested substance Pit (a)

30. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes: Endocytosis Figure 3.13a, step 6 Recycling of membrane and receptors (if present) to plasma membrane Cytoplasm Extracellular fluid Plasma membrane Detachment of vesicle Vesicle containing ingested material Vesicle Vesicle fusing with lysosome for digestion Release of contents to cytoplasm Lysosome Transport to plasma membrane and exocytosis of vesicle contents Plasma membrane Ingested substance Pit (a)

31. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes: Endocytosis Figure 3.13b–c

32. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes

33. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Cell Life Cycle  Cells have two major periods  Interphase  Cell grows  Cell carries on metabolic processes  Cell division  Cell replicates itself  Function is to produce more cells for growth and repair processes

34. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings DNA Replication  Genetic material is duplicated and readies a cell for division into two cells  Occurs toward the end of interphase  DNA uncoils and each side serves as a template

35. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings DNA Replication Figure 3.14

36. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Events of Cell Division  Mitosis—division of the nucleus  Results in the formation of two daughter nuclei  Cytokinesis—division of the cytoplasm  Begins when mitosis is near completion  Results in the formation of two daughter cells

37. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis  Prophase  First part of cell division  Centrioles migrate to the poles to direct assembly of mitotic spindle fibers  DNA appears as double-stranded chromosomes  Nuclear envelope breaks down and disappears

38. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis  Metaphase  Chromosomes are aligned in the center of the cell on the metaphase plate

39. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis  Anaphase  Chromosomes are pulled apart and toward the opposite ends of the cell  Cell begins to elongate

40. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis  Telophase  Chromosomes uncoil to become chromatin  Nuclear envelope reforms around chromatin  Spindles break down and disappear

41. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis  Cytokinesis  Begins during late anaphase and completes during telophase  A cleavage furrow forms to pinch the cells into two parts

42. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis Figure 3.15 Centrioles Plasma membrane Interphase Early prophase Late prophase Nucleolus Nuclear envelope Spindle pole Chromatin Centrioles Forming mitotic spindle Centromere Chromosome, consisting of two sister chromatids Fragments of nuclear envelope Centromere Spindle microtubules MetaphaseAnaphase Telophase and cytokinesis Daughter chromosomes Sister chromatids Nuclear envelope forming Nucleolus forming Spindle Metaphase plate Cleavage furrow

43. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis Figure 3.15, step 1 Centrioles Plasma membrane Interphase Nucleolus Nuclear envelope Chromatin

44. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis Figure 3.15, step 2 Centrioles Plasma membrane InterphaseEarly prophase Nucleolus Nuclear envelope Chromatin Centrioles Forming mitotic spindle Centromere Chromosome, consisting of two sister chromatids

45. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis Figure 3.15, step 3 Centrioles Plasma membrane InterphaseEarly prophaseLate prophase Nucleolus Nuclear envelope Spindle pole Chromatin Centrioles Forming mitotic spindle Centromere Chromosome, consisting of two sister chromatids Fragments of nuclear envelope Centromere Spindle microtubules

46. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis Figure 3.15, step 4 Metaphase Sister chromatids Spindle Metaphase plate

47. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis Figure 3.15, step 5 MetaphaseAnaphase Daughter chromosomes Sister chromatids Spindle Metaphase plate

48. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis Figure 3.15, step 6 MetaphaseAnaphaseTelophase and cytokinesis Daughter chromosomes Sister chromatids Nuclear envelope forming Nucleolus forming Spindle Metaphase plate Cleavage furrow

49. Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis Figure 3.15, step 7 Centrioles Plasma membrane Interphase Early prophase Late prophase Nucleolus Nuclear envelope Spindle pole Chromatin Centrioles Forming mitotic spindle Centromere Chromosome, consisting of two sister chromatids Fragments of nuclear envelope Centromere Spindle microtubules Metaphase Anaphase Telophase and cytokinesis Daughter chromosomes Sister chromatids Nuclear envelope forming Nucleolus forming Spindle Metaphase plate Cleavage furrow