1. 900-MHz MRI Probe for In Vivo Rats+: flexibility
safety
belts
respiratory
monitor
vacuum
exhaust
anesthesia
Bite bar
detachable
RF coils
animal
cradle
In vivo MRI of rodent heads
Fits up to 350-g rat
Animal is stabilized with bite bar and body restraints
Gaseous anesthesia & vacuum exhaust
Precise climate control
Respiration monitor
Rectal thermometer
ECG monitor
Detachable probe heads
Single or double resonance
Detachable RF probe heads
View of the rat cradle and RF coil

2. In vivo Adult Rat Images with 900-MHz Rat MRI Probe
Dr. Jeong-Su Kim, Department of Nutrition, Food and Exercise Sciences, FSU
In vivo 1H 2D Multi-slice FLASH (GRE).
TE = 4 ms, TR = 500 ms, NEX = 4, Acq time = 8.5 min,
Resolution = 234x469x500 μm, Fat Suppression.
In vivo 1H 2D Multi-slice RARE at 21.1 T. Large 350-g rat.
Top row: No fat suppression;
Bottom row: Fat suppressed.
Eff TE = 31 ms, TR = 3.5 s, NEX = 4, RARE factor = 4,
Acq time = 15 min, Resolution = 117x117x500 μm.
Aim: Evaluate an aged rat model to muscle and neurological disease of aging.

3. Ex vivo Adult Rat Head Images with 900-MHz Rat MRI Probe
Dr. Peter Thanos, Stony Brook University and Brookhaven National Laboratory
23Na 3D GRE at 21.1 T (ex vivo).
TE = 2.5 ms, TR = 50 ms, NEX = 10,
Acq time = 34 min, Iso. resolution = 500 μm.
1H 3D GRE of ex vivo rat head.
TE = 7.5 ms, TR = 150 ms, NEX = 2, Acq time = 12.5 h Isotropic resolution = 80 μm.
Aim: To correlate morphological changes in neuroanatomy with function PET during chronic exposure to mephamphetamines.

4. Chemotherapeutic Efficacy:
Implanted 9L Glioma with BCNU (2x) treatment
Drs. Victor Schepkin (NHMFL), Tom Chenevert & Brian Ross (U. Michigan)
In vivo ADC maps
Before Therapy
4 Days after Therapy
Aim: Utilize ADC and 23Na imaging as biomarkers for initial cancer screening, as well as quantification of therapeutic response & prediction of therapeutic efficacy.

5. Chemotherapeutic Efficacy:
Implanted 9L Glioma with BCNU (2x) treatment
Drs. Victor Schepkin (NHMFL), Tom Chenevert & Brian Ross (U. Michigan)
In vivo 23Na Images
Before Therapy
4 Days after Therapy
Aim: Utilize ADC and 23Na imaging as biomarkers for initial cancer screening, as well as quantification of therapeutic response & prediction of therapeutic efficacy.

6. Traumatic Brain Injury
Drs. Cathy Levinson (FSU College of Medicine) and Victor Schepkin (NHMFL)
in vivo Rat 1H MRI
in vivo Rat 21Na MRI
TBI
2D RARE: TR = 2 s; TE = 25 ms
Resolution = 0.1x0.1x0.5 mm
Time = 8 min
3D BP: TR = 100 ms; TE = 1 ms
Resolution = 0.75x0.75x0.75 mm
Time = 57 min
Aim: Utilize ADC and 23Na imaging to categorize the severity of injury and the impact of hormonal and dietary regulation in promoting TBI recovery.

7. Traumatic Brain Injury
Drs. Cathy Levinson (FSU College of Medicine) and Victor Schepkin (NHMFL)
RARE MRI
ADC Map
Aim: Utilize ADC and 23Na imaging to categorize the severity of injury and the impact of hormonal and dietary regulation in promoting TBI recovery.

8. High Resolution 1H MRI of Neurodegeneration K. Schweitzer, P
High Resolution 1H MRI of Neurodegeneration K. Schweitzer, P. Foroutan, D. Dickson, D. Broderick, U. Klose, D. Berg, Z. Wszolek, S. Grant Mayo Clinic-Florida, FSU and NHMFL
AIM: Utilize the SNR and contrast unique to high magnetic fields to examine postmortem human tissue representing different neurodegenerative diseases.
Demonstrating the first high resolution MR images of human hippocampal brain sections acquired at 21.1 T (900 MHz), this comparison presents hippocampal sections: a control (a) versus hippocampal sclerosis (b).
Control images display strong cell layer delineation, with hippocampal regions (CA 1-3) clearly visible. Sclerotic images lack hippocampal definition and displays significantly reduced volume and cell layer compression.

9. High Resolution 1H MRI of Neurodegeneration K. Schweitzer, P
High Resolution 1H MRI of Neurodegeneration K. Schweitzer, P. Foroutan, D. Dickson, D. Broderick, U. Klose, D. Berg, Z. Wszolek, S. Grant Mayo Clinic-Florida, FSU and NHMFL
AIM: Utilize the SNR and contrast unique to high magnetic fields to examine postmortem human tissue and neurodegenerative diseases.
Early Alzheimer’s Disease
Argyrophilic grain disease
&
Cortico-basal degeneration

10. The development of a novel sliding ring coil for 900 MHz
C. Qian, I. Masad, S. Grant and P. Gorkov
National Institutes of Health, Chemical & Biomedical Engineering,
The National High Magnetic Field Laboratory, The Florida State University
Sliding Ring design
1H Birdcage Coil (sliding ring)
for rat head imaging at 21.1 T
Standard design
Aim: To improve B1 uniformity over a range of potential user samples
for high field imaging applications

11. Sliding Ring Volume Coil for 21 T MRI
Broadband homogeneous tuning
Different loads – birds to rat heads
Symmetric tuning with sliding ring
Fixed field of view
Single & double resonant prototypes
Good isolation without using baluns 1H
Single resonance 1H
23Na
Double resonance 1H/23Na
Single resonance 1H rat head SR coil

12. Dielectric Resonators for MR Microscopy at 21.1 T
User Collaborative Group Project (UCGP)
SC Grant, Chemical & Biomedical Eng, FSU
AG Webb, Bioengineering, Penn State U.
Dielectric resonators (DRs), which are compact magnetic field storage devices that typically operate in the GHz range, are a promising new approach for high-field MR applications. In fact, the higher the frequency, the more compact the DR and easier the manufacturing. The use of high permittivity, low loss materials yields DRs with Q values much greater than 1000, which translate to higher signal-to-noise ratios for MR applications.
AIM: Test DRs versus similarly sized copper coils for MR applications at 900 MHz (21.1 T) with respect to sensitivity & RF homogeneity.
DR resonance is tuned using permittivity & dimensions
Previous publications by collaborators: T. Neuberger, V. Tyag, E. Semouchkina, M. Lanagan, A. Baker, K. Haines, A.G. Webb. Concepts in Magnetic Resonance Part B (Magnetic Resonance Engineering), Vol. 33B(2) 109–114 (2008)
K. Haines, et al. J. Magnetic Resonance. In press
4.76 mm
radius
28 mm
14 mm
height
21.1-T CaTiO3 DR mm 3D MR images of excised mouse spinal cord (L) & rat muscle

13. Dielectric Resonators for MR Microscopy at 21.1 T
B1 Maps
Graduate students
Kristina Haines (PSU) &
Jose Muñiz (FSU) and their work are supported by the UCGP & NHMFL
Based on 21.1 T results, DRs will be extended to the 25-T Keck resistive and SCH magnets.
Dielectric AG LG

14. Dielectric Resonators: Science Enabled
Fly Samples: B. Null, Stanford DR: A. Webb, H. Haines, M Lanagan, PSU
J. Muniz, S. Grant, FSU
Larva Late-stage pupa
Adult Fly
3D FLASH acquired with 900-MHz
CaTiO3 dielectric resonator
30-micron isotropic resolution
temp = 4 C
Acquisition time ~ 1 hr
<= 3D rendering of adult fly

15. Bimodal Intracellular In/P Quantum Dots for MRI J. Rosenberg, J
Bimodal Intracellular In/P Quantum Dots for MRI J. Rosenberg, J. Kogot, D. Lovingood, G. Strouse, S. Grant, FSU and NHMFL
Multimodal, bio-compatible contrast agents for high magnetic field applications represent a new class of nanomaterials with significant potential for tracking of fluorescence and MR contrast in vitro and in vivo.
AIM: Optimized for high field MRI, this nanoparticle capitalizes on the improved performance of chelated Dy3+ with increasing magnetic field coupled to a non-cytotoxic InP/ZnS quantum dot
fluorescence detection
MR responsiveness
payload delivery

16. 1Chemical & Biomedical Engineering, The Florida State University
Intracellular MRI Contrast by SPIOs at and 21.1 T
J.T. Rosenberg1,2, J. Kogot3, C. Ridel3, G. Strouse3 and S.C. Grant1,2
1Chemical & Biomedical Engineering, The Florida State University
2The National High Magnetic Field Laboratory, 3Chemistry & Biochemistry, The Florida State University
Aim: To assess the sensitivity of SPIO agents as a function of field strength
Quantitative relaxation measurements were made on SPIO loaded Bv2 rat neuroglia cells, varying either the agent used to dose the cells or the number of cells immobilized in agarose.
Cell-Dosing
11.75T
21.1T