1. Ribosomes
Mol. Biol. of the Cell

2. Prokaryotic ribosome
Mol. Biol. of the Gene

3. Eukaroytic ribosome
Mol. Biol. of the Gene

4. Sedimentation of ribosomes
Mol. Biol. of the Gene

5. The „ribosome cycle“
Mol. Biol. of the Gene

6. Polyribosomes (polysome)
Mol. Biol. of the Gene

7. Peptidyl transferase reaction
Mol. Biol. of the Gene

8. tRNA binding sites
Mol. Biol. of the Gene

9. Overview on translation initiation
Mol. Biol. of the Gene

10. Ribosome binding site (Shine-Dalgarno sequence) on mRNA
Mol. Biol. of the Gene

11. Internal start site are important for polycistronic mRNAs
Mol. Biol. of the Cell

12. N-formyl methionine
Mol. Biol. of the Gene

13. Initiation factors bound to the ribosome
Mol. Biol. of the Gene

14. Sequence of events during initiation in prokaryotes
Mol. Biol. of the Gene

15. Capping
Mol. Biol. of the Gene

16. Translation initiation in eukaryotes
Mol. Biol. of the Gene

17. Start codon identification in eukaryotes
Kozak sequence at start codon:
G/ANNAUGG
Mol. Biol. of the Gene

18. Circular polysomes in eukaryotes
Mol. Biol. of the Gene

19. Circular polysomes
Mol. Biol. of the Cell

20. Short upstream ORFs in eukaryotes
Mol. Biol. of the Gene

21. Internal ribosome entry sites in eukaryotes (IRES)
Mol. Biol. of the Gene

22. Overview of the elongation phase
Mol. Biol. of the Gene

23. Binding of aa-tRNA to the A-site
EF-Tu:
protection of ester from hydrolysis
check for correct codon recognition
Mol. Biol. of the Gene

24. Codon recognition by the anticodon
Mol. Biol. of the Gene

25. EF-Tu GTP hydrolysis in codon recognition
Mol. Biol. of the Gene

26. Accommodation as the second recognition control step
Mol. Biol. of the Gene

27. Translocation
Mol. Biol. of the Gene

28. Class I (stop codon recognition) prokaryotic: RF1: UAG, UAA
Release factors
Class I (stop codon recognition)
prokaryotic:
RF1: UAG, UAA
RF2: UGA, UAA
eukaryotic:
eRF1: UAA, UAG, UGA
Class II (diss. of class I factors)
RF3
eRF3
Mol. Biol. of the Gene

29. Action of release factors
Mol. Biol. of the Gene

30. Ribosome recycling factor
Mol. Biol. of the Gene