2. Pharmacology of the Endocrine System

3. Topics Pituitary and hypothalamic hormones Pituitary and hypothalamic hormones Thyroid and antithyroid Drugs Thyroid and antithyroid Drugs Adrenal Drugs Adrenal Drugs Pancreatic Drugs Pancreatic Drugs Gonadal hormones and inhibitors Gonadal hormones and inhibitors

4. The Endocrine System Controls many body functions Controls many body functions –exerts control by releasing special chemical substances into the blood called hormones –Hormones affect other endocrine glands or body systems

5. Endocrine System

7. Hormone A substance that is released in one tissue and travels through the circulation (usually) to the target tissue. A substance that is released in one tissue and travels through the circulation (usually) to the target tissue. Hormones reach all parts of the body, but only target cells are equipped to respond Hormones reach all parts of the body, but only target cells are equipped to respond Hormones are secreted in small amounts and often in bursts (pulsatile secretion)

8. Endocrine Functions Maintain Internal Homeostasis Support Cell Growth Coordinate Development Coordinate Reproduction, fertility, sexual function Facilitate Responses to External Stimuli

9. 4 Classes of Hormones 1. Peptide/ Protein (Range from 3 amino acids to hundreds of amino acids in size. ) 2. Steroid 3. Amine (Thyroid hormones and Catecholamines) 4. Eicosanoid (Fatty acid derivatives )

10.   The hormones fall into two general classes based on their solubility in water.   The water soluble { amine (epinephrine) and peptide/protein hormones} are secreted by exocytosis, travel freely in the bloodstream, and bind to cell-surface receptors.   The lipid soluble hormones { thyroid hormone, steroid hormones and Vitamin D3}. diffuse across cell membranes, travel in the bloodstream bound to transport proteins, and diffuse through the membrane of target cells.

11. NUCLEUS Signal receptor (a)(b) TARGET CELL Signal receptor Transport protein Water- soluble hormone Fat-soluble hormone

12. Location of receptors - On cell surface Peptides and proteins Peptides and proteins - In cytoplasm Steroids Steroids - In nucleus Thyroid hormones Thyroid hormones

13. Mechanisms of endocrine disease Endocrine disorders result from: Hormone deficiency,(HRT) Hormone excess (Specific antagonists or release inhibitors ) Hormone resistance (sensitizers (sensitizers)

14. Sources of hormones: - - Natural Human (GH; LH & FSH; hCG); Animal (Insulin, T 3 & T 4 ) - - Biosynthetic Insulin (Porcine & Bovine) - - Synthetic Most hormones and their antagonists DNA recombinant technology

15. Hypothalamic & Pituitary Hormones

16. Spinal cord Posterior pituitary Cerebellum Pineal gland Anterior pituitary Hypothalamus Pituitary gland Hypothalamus Thalamus Cerebrum

17. Hypothalamus and Pituitary The output of the hypothalamus-pituitary unit regulates the function of the thyroid, adrenal and reproductive glands and also controls somatic growth, lactation, milk secretion and water metabolism. Hypothalamic Hs can have effect of stimulating or inhibiting the release of ant. Pit. Hs. Called RELEASING HORMONES “RH” or INHIBITING HORMONES “IH” respectively, reflecting their influence on ant. Pit. Hs.

18. The Pituitary Gland The Pituitary Gland is divided into 2 areas, with separate types of hormone production. The anterior pituitary makes and releases H under regulation of the hypothalamus   Growth Hormone (GH)   Thyroid-stimulating Hormone (TSH)   Adrenocorticotropin (ACTH)   Follicle-stimulating Hormone (FSH) ),   Leutinizing Hormone (LH),   Prolactin – –The posterior pituitary stores and secretes H that are made in the hypothalamus: oxytocin and antidiuretic hormone (ADH)

20. Hypothalumus Ant. pituitary Post. Pituitary Growth hormone-releasing hormone(GHRH) + Growth Hormone (GH), Antidiuretic Hormone Antidiuretic Hormone Growth hormone-releasing inhibiting hormone (GHRIH)_ Somatostatin Somatostatin Thyrotropin-releasing hormone (TRH)+ Thyroid Stimulating Hormone (TSH), Oxytocin Corticotropin-releasing hormone (CRH)+ Adrenocorticotropic Hormone (ACTH), Gonadotropin-releasing hormone (GnRH)+ Follicle-stimulating Hormone (FSH), Luteinizing Hormone(LH). Dopamine _ Prolactin,

21. Hypothalamic hormones: 1- Growth Hormone- Releasing Hormone(GHRH): Together with somatostatin controls release of the GH from the ant. pit. Together with somatostatin controls release of the GH from the ant. pit. It is released from hypothalamus in a pulsatile fashion, with 5-9 major pulses detected per day. GHRH release is enhanced by α2-adrenergic agonists (e.g. clonidine) and opioids. GHRH release is increased by vigorous exercise.

22. Diagnostic Uses of GHRH (Sermorelin) To test pituitary function in patients with GH deficiency. GH deficiency could reflect either a hypothalamic or a pit. defect. GH deficiency could reflect either a hypothalamic or a pit. defect. If the primary defect is hypothalamic, as is most common, GHRH will elicit an increase in GH release. If the primary defect is hypothalamic, as is most common, GHRH will elicit an increase in GH release. If the defect is at the level of the pituitary, there will be no increase in GH following GHRH administration. If the defect is at the level of the pituitary, there will be no increase in GH following GHRH administration. Therapeutic Uses of GHRH to enhance GH secretion Pulsatile subcutaneous delivery of GHRH, mimicking the normal endogenous patterns (e.g. ~ every 3 hours) has been used to stimulate GH release in patients with GH deficiency that is not of pituitary origin. IV, SC, intranasal IV, SC, intranasal

23. Inhibits GH release and TSH from the ant. pituitary. Inhibits release of most GI hormones, reduces gastric acids and pancreatic secretion. (glucagon, insulin & gastrin), Therapeutic Uses Somatostatin Somatostatin is of no clinical value because of it's short half-life (<3 min) Octreotide,a synthetic somatostatin analogue with a longer duration of action Lanreotide is much longer acting, and is administered only twice a month. used to treat: Acromegaly, Gastrinoma,Glucagonoma & Other Endocrine Tumors, and esophageal varices bleeding. ( Inhibits Mesenteric vasodilatation induced by glucagon) A/E: GI disturb. postprandial hyperglycemia. Gall stones often form as a result of decreased biliary flow and gall bladder contraction. 2- Somatostatin (Growth hormone-releasing inhibiting hormone (GHRIH):

24. 3- Thyrotropin-Releasing Hormone(TRH): Stimulates release of thyrotropin (TSH) from the ant pit. Is used in diagnostic testing of thyroid dysfunction Protirelin: IV 4- Corticotropin Releasing Hormone(CRH) It It stimulates secretion of both ACTH & beta –endorphin (a closely related peptide ) from the ant. pituitary. CRH can be used in the diagnoses of abnormalities of ACTH secretion.

25. 5- Gonadotropin-Releasing Hormone:(GnRH): Stimulate the gonadotroph cell to produce and release LH and FSH, Stimulate the gonadotroph cell to produce and release LH and FSH, Gonadorelin, Buserelin, Nafarelin Gonadorelin, Buserelin, Nafarelin GnRH agonists, SC infusion in pulses mimic physiological GnRH, stimulates ovulation. GnRH agonists, SC infusion in pulses mimic physiological GnRH, stimulates ovulation. In contrast, steady dosing inhibits gonadotropin release by causing down –regulation (desensitization) of GnRH receptors in pituitary cells that normally release gonadotropins. In contrast, steady dosing inhibits gonadotropin release by causing down –regulation (desensitization) of GnRH receptors in pituitary cells that normally release gonadotropins. GnRH is used in the diagnosis & treatment (by pulsatile administration) of hypogonadal states in females & males. GnRH is used in the diagnosis & treatment (by pulsatile administration) of hypogonadal states in females & males. Continuous GnRH agonists are used in sex H-dependent conditions: prostate & breast cancers, uterine fibroids, endometriosis or precocious puberty. Continuous GnRH agonists are used in sex H-dependent conditions: prostate & breast cancers, uterine fibroids, endometriosis or precocious puberty.

26. 6- Prolactin-Inhibiting Hormone (PIH,dopamine): Dopamine is the physiologic inhibitor of prolactin release Dopamine is the physiologic inhibitor of prolactin release Because of its peripheral effects & the need for parenteral administration, dopamine is not useful in the control of hyperprolactinemia, but bromocrptine & other orally active ergot –derivatives (eg. Cabergoline, pergoline) are effective in reducing prolactin secretion from the normal glands as well as from prolactinomas. Because of its peripheral effects & the need for parenteral administration, dopamine is not useful in the control of hyperprolactinemia, but bromocrptine & other orally active ergot –derivatives (eg. Cabergoline, pergoline) are effective in reducing prolactin secretion from the normal glands as well as from prolactinomas. Also used in treatment of acromegaly Also used in treatment of acromegaly A/E: orthostatic hypotension, Psychiatric manifestations

27. Pituitary Hormones

28. Anterior pituitary Hormones

29. Growth Hormone Derived from the somatotroph cells Its secretion is controlled by GHRH and somatostatin; GH secretion is high in newborn, deceasing at 4 yr to an intermediate level, which is then maintained until puberty, when there is further decline. Insulin-like growth factor 1 (IGF-1) released from the liver inhibits GH secretion by stimulating somatostatin secretion from the hypothalamus,

30. Growth Hormone Activity 1.Increases plasma free fatty acids (source of energy for muscle tissue) 2.Increases hepatic glucose output 3.Decreases insulin sensitivity in muscle 4.Is protein anabolic hormone Growth Hormone Deficiency Can have a genetic basis or can be acquired as a result of damage to the pituitary or hypothalamus by a tumor, infection, surgery, or radiation therapy. In childhood: short stature and adiposity, hypoglycemia. Adults : generalized obesity, reduced muscle mass.

31. GROWTH HORMONE EXCESS Mainly benign pituitary tumor In adults causes acromegaly, If this occurred before the long bone epiphyses close, it leads to the rare condition, gigantism. Treatment of excess GH disorders: - Synthetic Somatostatin (Octreotide) - DA agonists (Bromocriptine) - Surgical removal / Radiotherapy of the tumor - GH Antagonists (Pegvisomant)

32. An excess of GH can cause gigantism, while a lack of GH can cause dwarfism An excess of GH can cause gigantism, while a lack of GH can cause dwarfism

33. Clinical uses of GH Somatotropin GH deficiency in children & adults. GH deficiency in children & adults. Children with short stature that is due to factors other than GH deficiency: Children with short stature that is due to factors other than GH deficiency:  Idiopathic short stature, Turner syndrome, Chronic renal failure A/E: Hypothyroidism, Pancreatitis, Gynecomastia, Hypothyroidism, Pancreatitis, Gynecomastia, Possibilities of abuse have also arisen, e.g. creation of “super” sports people.

34. Thyroid-stimulating Hormone (TSH) Also called thyrotrophin Also called thyrotrophin Stimulates secretion of thyroid hormone & growth of thyroid gland. Diagnostic Uses of TSH In patients who have been treated surgically for thyroid carcinoma, to test for recurrence In patients who have been treated surgically for thyroid carcinoma, to test for recurrence

35. Adrenocorticotropin (ACTH) Stimulates cortisol secretion by the adrenal cortex & promotes growth of adrenal cortex Diagnostic use Diagnostic use : as a test of the capacity of the adrenal cortex to produce cortisol;

36. Follicle – stimulating hormone (FSH) Females: stimulates growth & development of ovarian follicles, promotes secretion of estrogen by ovaries. Males: required for sperm production 3 preparations are available for clinical use: Urofollitropin,purified from of the urine of post menopausal women, 2 recombinant forms, follitropin alpha & follitropin beta. These products are used in combination with other drugs to treat infertility in women & men.

37. Leutinizing hormone (LH) Females: responsible for ovulation, formation of corpus luteum in the ovary, and regulation of ovarian secretion of female sex hormones. Males: stimulates cell in the testes to secrete testosterone.Lutropin alfa, approved for use in combination with follitropin alfa for stimulation of follicular development in infertile women with profound LH deficiency.

38. Prolactin Secreted by lactotroph cells of the ant. Pit., which increase in number during pregnancy. Its secretion is stimulated by estrogen Females: stimulates breast development and milk production. Males: involved in testicular function No preparation of prolactin is available for use in prolactin- deficient patients. For patients with symptomatic hyperprolactinemia, inhibition of prolactin secretion can be achieved with dopamine agonists, which act in the pituitary to inhibit prolactin release.

39. Posterior pituitary Hormones

40. Oxytocin It is synthesized in the hypothalamus & transported to the post. Pit. It is synthesized in the hypothalamus & transported to the post. Pit. It is an effective stimulant of uterine contractions & is used intravenously to induce or reinforce labor. It is an effective stimulant of uterine contractions & is used intravenously to induce or reinforce labor. Induces the release of milk Induces the release of milk Suckling sends a message to the hypothalamus via the nervous system to release oxytocin, which further stimulates the milk glands Suckling sends a message to the hypothalamus via the nervous system to release oxytocin, which further stimulates the milk glands

41. Clinical uses of oxyticin IV, IM Induction of labor Induction of labor Control of postpartum bleeding Control of postpartum bleeding A/E : fetal distress, placental abruption, or uterine rupture excessive fluid retention

42. Vasopressin (antidiuretic hormone ADH) It is synthesized in the hypothalamus & transported to the post. Pit. It is synthesized in the hypothalamus & transported to the post. Pit. The function of ADH is to increase water conservation by the kidney. The function of ADH is to increase water conservation by the kidney.  If there is a high level of ADH secretion, the kidneys reabsorb water.  If there is a low level of ADH secretion, the kidneys release water in dilute urine. ADH release increases if blood pressure falls or blood becomes too salty. ADH causes peripheral blood vessel constriction to help elevate blood pressure.

43. Clinical uses Diabetes insipidus, Diabetes insipidus, Nocturnal enuresis (by decreasing nocturnal urine production) Nocturnal enuresis (by decreasing nocturnal urine production) A/E: hyponatremia and seizures Synthetic ADH drugs – Vasopressin: IV, IM –Desmopressin: IV, IM. PO, intranasal