1. Receptors and transduction 1 References: Chapter 11 – Neuron by Levitan & Kaczmarek OR Chapter 6 – Neuroscience by Purves et al 1.K. Tsuzuki and T. Ozawa. Glutamate receptors www.els.net 2.Madden, D. Structure and function of glutamate receptor ion channels (2002). Nature Revs Neurosci. 3, 91. Dr. MV Hejmadi

3. Synapse Synapse: the point where electrical conduction is converted to chemical conduction (mostly)

4. The Chemical Synapse 1) AP invades 2) Ca ++ influx 3) Depolarization  release of NT 4) NT diffusion 5) Ligand binding 6) Depolarization 7) transmitter recycling 8) vesicular membrane recycling

5. Action potential Calcium channels Calcium ions L-glutamate vesicles L-glutamate release AMPAR Na + NMDAR Mg 2+ Synaptic cleft Presynaptic Terminal Postsynaptic spine Excitatory synaptic transmission by L-glutamate

6. Glutamate Acetylcholine etc GABA

7. Specificity of responses Following activation by a NT, how does a neuron know what the response should be? Depending on the type of NT (excitatory or inhibitory), key receptors transduce this signal and dictate the nature of the response

8. Glu DA ACh Substance P GABA Output i.e. glutamatergic, GABAergic, cholinergic etci.e. glutamatergic, GABAergic, cholinergic etc Each neuron uses one NT to send a signal (usually) Synaptic inputs to a GABA-ergic medium spiny neurone

9. Each NT can activate diverse receptors e.g. acetylcholine can activate 2 classes of receptors, nicotinic or muscarinic Nicotinic AChR Mimicked by nicotine (agonist) Only in certain tissues (skeletal muscle, parts of the CNS and PNS) Muscarinic AChR Mimicked by muscarine (agonist) Cardiac muscle, cholinergic synapses of the CNS

10. How do you measure specificity? Pharmacological tools Nicotinic AChR blocked by antagonists  -bungarotoxin curare Muscarinic AChR Blocked by antagonists like atropine Bungarus multicinctus Henbane plant

11. Receptors categorised based on their transduction mechanisms A)Ionotropic receptors A)Ionotropic receptors Ionotropic receptors are directly coupled to ion channels and are activated by neurotransmitters which open the channel pore to allow movement of specific ions, creating postsynaptic potentials B) Metabotropic receptors B) Metabotropic receptors These receptors are not directly coupled to their ion channels and transduce the signal via guanyl nucleotide-binding proteins (G-proteins) that activate intracellular second messenger pathways FAST DIRECT SLOW INDIRECT PSPs

12. Fig 5.22; Purves et al FAST DIRECT PSPs SLOW INDIRECT PSPs

13. Ionotropic receptors Fig 11.8; Levitan and Kaczmarek

14. Ionotropic receptor types pentameric glutamate tetrameric Assembly of either similar subunits (homomeric) or different subunits (heteromeric)

15. Heterogeneity of nAChR in mammalian tissuesBrain addiction analgesia anxiety attention cognition  AutonomicGanglia cardiovascular gastro-intestinal  Muscle contraction                 

16. Ionotropic receptors - Generic structure Example: Nicotinic acetylcholine receptor (pentameric) Subunits Each subunit has four hydrophobic regions (TMI – IV)

17. glutamate Ionotropic receptors-Generic structure Unlike other iR, iGluR have a re-entrant loop inTMII domain extracellular N-terminus and intracellular C- terminus Long loop between TMIII and IV forms part of the binding domain with the C- terminal half of the N- terminus

18. The modular nature of iGluR subunits Extracellular NTD followed by S1 - sequence between NTD and TMI S2 - sequence between M3 and M4 TMIII and TMIV domains with an intervening re-entrant P loop,. C terminus intracellular S1 and S2 half-domains form the ligand-binding domain. The structure of this domain is shown as a ribbon diagram. It consists of two lobes (lobe I, blue; lobe II, red), separated by a ligand-binding cleft. Lobe 1- structural unit attached to the amino-terminal domain Lobe 2 - structural unit that links to M1 and M3 Madden, D. Structure and function of glutamate receptor ion channels (2002). Nature Revs Neurosci. 3, 91.

19. Modular structure of iGluRs Emerging structural explanations of ionotropic glutamate receptor function ROBERT L. McFEETERS and ROBERT E. OSWALD The FASEB Journal. 2004;18:428-438 transmembrane topology, the two extracellular domains, and the carboxyl-terminal domain. Flip/flop refers to an alternatively spliced segment that plays a role in desensitization. S1 refers to the sequence between the amino-terminal domain and M1; S2 refers to the sequence between M3 and M4. Lobe 1 is the structural unit attached to the amino-terminal domain, and Lobe 2 is the structural unit that links to M1 and M3. Note that segments of S1 and S2 are found in both Lobes 1 and 2.

20. Monomers associate most strongly through interactions between their amino-terminal domains (NTDs) (star in middle figure). Dimers undergo a secondary dimerization, mediated by interactions in the S2 and/or transmembrane domains (stars in right-hand figure). The crystallographically observed S1S2 dimer probably corresponds to this secondary dimerization interaction. The 'dimer-of-dimers' model of iGluR assembly

21. NMDA receptor (N-methyl- D-apartate) AMPA receptor (  -amino 3hydroxy 4methyl 5propionic acid) Kainate receptors (kainic acid) Epileptogenesis Synaptic plasticity? Flip/flop region- spliced variants (exon 14 –flop and Exon 15 for flip) Modulates LTP Results in calcium entry, changes in secondary messages altered protein synthesis

22. Calcium as a signal The NMDA receptor is blocked by a Mg++ ion at resting potential Glutamate directly gates NMDA and AMPAR. NMDAR controls movement of Ca 2+ Na + and K+ binding sites for glycine, zinc, phencyclidine (angel dust) and Mg 2+. Under resting conditions Mg 2+ blocks the site and keeps the receptors silent. Upon depolarisation the Mg is released and there is movement of Ca into the cell.

23. NMDA receptors GLYCINEGLUTAMATE Mg MK801 NR1-1a/b-4a/b NR2A NR2B NR2C NR2D NR3A Ca Na calmodulin PSD proteins CamKII P P NR1NR2 2+ + OUT IN P = multiple sites for phosphorylation Zn 2+ POLYAMINE NR1 NR2 ?

24. So what are the most important NT in the mammalian brain? glutamate and GABA are the most abundant which mediate synaptic transmission in the CNS via ionotropic receptors (LGICs) In general, GABA - inhibitory whereas glutamate - excitatory

25. Roles of glutamate in the CNS Synaptic transmission (EPSPs) Long term depression Long term potentiation –Dendritic sproutin –Synaptic modification –Control of gene expression Excess of glutamate release can cause neuronal death (excitotoxicity) e.g. during stroke, epilepsy, Parkinson’s disease

26. Effects of glutamate-induced excitotoxicity Nature Reviews Neuroscience 4; 399-414 (2003

27. GABA receptors GABA - major inhibitory NT in the mammalian CNS GABA receptors are pentameric in structure 3 classes GABA A and GABA C receptors are ionotropic, GABA B receptors are metabotropic

28. GABA A receptor binding sites Benzodiazepine – allosteric agonist - tranquilisers / anticonvulsants Barbiturates – prolong action of GABA - anaesthetics / hypnotics

29. So why have multiple iR? The post-synaptic response to stimulation can be modulated in the short term (i.e. for hundreds of milliseconds) or for the long term (hours, days or even weeks!!). Synaptic strength is increased or decreased by altering the level of post-synaptic depolarisation. This is achieved through changing how well receptors respond to stimulation, – by altering the length of time they are active, –the number of receptors physically present or –by altering the amount of L-glutamate that is released into the synaptic cleft