1. LB145 Sec 001-004

2. Today’s Outline HAND IN Homework1– front of classroom. StudyNotes2 due on Thursday – see website. Announcements: – LB145 Tutorial! – Field Trip! Review: Membranes Prokaryotes versus Eukaryotes Organelles – Nucleus, RER, Golgi Apparatus, Cytoskeleton, SER, Ribosomes, Lysosomes, Peroxisomes, Vacuoles, Mitochondria, Chloroplasts Evaluate Homework

3. Announcement 1: LB145 Tutorial Purpose: to help students catch up! – Weak chemistry background. – A bit lost with where we are right now. Format: – Teams of 2-4. Working on problems with the help of two senior LAs. (subject to change) Friday 3-5pm in Lab room C-3. Accommodates 20-25. Email me to reserve a place – otherwise, first come, first serve.

4. Announcement 2: Field Trip Field Museum in Chicago Friday March 22 nd : 7am – 12 midnight Honors option for this course: the biology of bioluminescence. Open to all students, but honors-option students allowed to sign up first. Remaining places determined by lottery. Cost: ~$12 per student.

5. Which of the following statements about saturated phospholipids is true? a)They are more common in plants than animals. b)They have double bonds in the carbon chains of their fatty acids. c)They are more fluid at room temperature d)They contain more hydrogen that unsaturated phospholipids. e)They make membranes more permeable. Saturated vs. Unsaturated

6. The Shape of Phospholipids… Why is one leg of the phospholipid bent?

9. Forging Ahead: Cells and Organelles

10. Prokaryotes vs Eukaryotes

11. Prokaryotes  Structure Single celled Plasma membrane Cell wall Cilia/flagella (sometimes) Simple cytoskeleton (sometimes)  Other features Plasmids Ribosomes Single circular chromosome Organelles (few and only sometimes)

13. Eukaryotes  Structure Single or multicellular Membrane and wall (sometimes) Cytoskeleton  Other features Nucleus Ribosomes Many chromosomes Extensive internal membranes… Membrane bound organelles

14. Prokaryotes vs Eukaryotes

15. -Nucleus -Ribosomes -Rough Endoplasmic Reticulum (RER) -Smooth Endoplasmic Reticulum (SER) -Golgi Apparatus -Peroxisomes -Lysosomes -Vacuoles -Mitochondria -Chloroplasts -Cytoskeleton Eukaryotes: Organelles to Live By

16. Discuss: Pick three organelles and write down everything you know about them. Turn to your neighbor and compare your lists. Debrief –

17. The Nucleus

18. Campbell 8e, Fig. 6.10 The Nucleus

19. The Nuclear Envelope n Allows movement of molecular structures into and out of the nucleus mRNA and other molecules move to the cytoplasm for encounters with ribosomes Proteins, both structural and regulatory, are shipped into the nucleus

20. mRNA is made in the nucleus (Transcription) n mRNA molecules move to the cytoplasm where they encounter ribosomes n Together, mRNA and ribosomes make proteins (Translation)

21. Ribosomes

22. Ribosome and rRNA rRNA is made in the nucleus. Small and Large Subunit proteins are transported INTO the nucleus. Where the ribosome is assembled. (below: protein in purple, rRNA in orange and yellow) Eukaryotes have FOUR strands of rRNA. 5S 5.8S 28S 18S Prokaryotes have THREE strands of rRNA. 5S 23S 16S

23. rRNA and tRNA are made in the nucleus (Transcription) http://alienvisions.blogspot.com/ n rRNA molecules are packaged with proteins to make ribosomal subunits Nucleolus site of synthesis Ribosomal subunits move to cytoplasm through nuclear pores n tRNA molecules move to the cytoplasm and helps translate mRNA into protein

24. Keeping RNAs Straight mRNA – MESSANGER RNA – Transcribed from DNA in nucleus. – Transported out into cytosol. – Interacts with ribosome where translation takes place and a protein is formed. rRNA – RIBOSOMAL RNA – Transcribed from DNA in nucleus – Binds to large and small ribosomal subunits in nucleus – acts as structural support. – Exits nucleus (bound to ribosomal subunits) via nuclear pore. tRNA – TRANSFER RNA – Transcribed from DNA in nucleus – Exits into cytosol via nuclear pore – Picks up a specific amino acid (covalent bond) in cytosol and transfers it to growing polypeptide chain.

25. Write it Down: How many different kinds of mRNA do we have? How many different kinds of rRNA do we have? How many different kinds of tRNA do we have?

26. Coming Up: StudyNotes #2 is due on Thursday – StudyNotes #2 is due on Thursday. It’s a big one! 7.0, 7.1, 7.2, 7.5 – If you have Freeman 3, the sections are different – see website. Homework #2 (Cystic Fibrosis) will be posted tomorrow. (due Tues Jan. 22) Exam #1 is on the 24 th !