1. The VANASYL Bags Vanasyl LLC.

2. Areas for Bag Technology Applications  Bioreactor sampling  Bioreactor additions (antifoam etc.)  Media/buffer prep & storage  Product storage & transfer  Lab & pilot scale bioreactors  Aseptic connections

3. Vanasyl Bag Material: Multilayer Ethylene-Vinylacetate (EVA) With A Barrier Film. (Film Gauge 400 μm @ 23°C) Examples of Suitable Uses: Cell Culture Amino Acids Lipophylic Solutions Product Storage and Transportation Medium Storage and Delivery Sampling Collection and more.

4. Issues with Accessing Samples in Bottles and Non-Vanasyl Bags  Requires Safety Cabinet  Requires replacing a Bottle with a Septum Risks contamination Risks contamination Time Consuming Time Consuming  Sample withdrawal requires syringe with needle Needle-stick risk to personnel Needle-stick risk to personnel Insurance factor Insurance factor  Time required compromises sample viability pH change pH change Contamination Contamination

5. Benefits of Vanasyl Bags  No needles- eliminating needle injuries and disposal issues.  No need for crimping- minimizing human error.  No need for a safety cabinet.  No need to empty the bag.  Faster sampling. A sample can be taken, anywhere from 10 to 30 seconds after filling the sampling bag via a sterile syringe- ensuring sample integrity.  Bag can be stored, then frozen down to 90°C, then brought back to room temperature without glass transition and samples can continue being taken.  Pre-sterilized (gamma-irradiated).  No particles.  No leakage.  Non-fragile.

6. Technical Data (more available upon request) Physical PropertiesDimensionValueProcedure Water Vapor Transmission Rate g/(m2.day.bar)1.6ASTM F- 1249 Oxygen Permeabilitycc(m2.day.bar).0000022ASTM D- 3985 CO2 Permeabilitycc(m2.day.bar).000006Mocon Permatran C-IV Cryogenic SuitabilityCelsius<-90

7. UV Transmission Rate

8. Available Bag Sizes   150cc, 250cc, 500cc, and 1,000cc. (As standard with the Clave® fitting; otherwise to the end user’s specifications)   2L to 25L and larger (The exact bag size, tubes, and fittings are to the end user’s specifications )

9. CONTACT INFO Vanasyl LLC. Director: Tamara Fridman 347-675-5452