1. Chapter 8 Cell Adhesion Molecules (CAM)

2. Chapter 8 Cell Adhesion Molecules
Contents
PartⅠ Membrane molecules of immune cell
PartⅡ Definition of leukocyte differentiation
antigen and CD
PartⅢ Definition, classification and functions
of adhesion molecules
PartⅣ Clinical application of CD and
adhesion molecules

3. PartⅠ Membrane molecules of immune cell
Receptors: TCR, BCR, CR, CKR, FcR
ClassⅠand classⅡ MHC molecules
CD molecules: CD1~339
Cell Adhesion Molecules

4. PartⅡ Definition of leukocyte differentiation antigen and CD
Leukocyte differentiation antigen: Cell surface molecules expressed (or disappeared) during different developmental and differential phases, activation or inactivation process of blood cells.

6. PartⅡ Definition of leukocyte differentiation antigen and CD
CD: cluster of differentiation.
The same differentiation antigen recognized by different monoclonal antibody from different lab are called CD.

7. CDs which take part in T cell recognition, adhesion and activation

8. CDs which take part in B cell recognition, adhesion and activation

9. PartⅢ Definition, classification and functions of adhesion molecules

10. Ⅰ. Definition
Cell adhesion molecules (CAMs) are cell surface proteins involved in the interaction of cell-cell or cell-extracellular matrix.
CAMs take effect by the binding of receptor and ligand.

11. Ⅱ. Classification Integrin family Selectin family
Immunoglobulin (Ig) superfamily
Cadherin family
Mucin-like family
Other adhesion molecules

12. 1. Integrin family Integrins consist of α and β chains.
According to β subunits, Integrins are divided into eight groups: β1- β8
VLA-4(Very Late Antigen-4)------VCAM-1
LFA-1(Lymphocyte Function-associated Antigen-1)------ICAM-1,2,3
MAdCAM-1 (Mucosal Addressin Cell Adhesion Molecule-1)
TSP-1 ((Thrombospondin一1):凝血酶敏感蛋白一1

17. Integrin and platelet aggregation
(Karp, 2001)

18. Integrins promote immune response

19. 2. Selectin family Selectins consist of one peptide chain.
The three family members include: E-selectin, L-selectin, and P-selectin.

24. 3. Ig superfamily(IgSF)
The structure of these adhesion molecules resemble that of Ig.
CD4, CD8, CD2(LFA-2), CD58(LFA-3), VCAM-1, ICAM-1,2,3

30. 4. Cadherin family E-cadherin------ Epithelia cell
N-cadherin Nerve cell
P-cadherin Placenta

32. 5. Mucin-like family CD34, GlyCAM-1(glycosylation dependent cell adhesion molecule-1) PSGL-1(P-selectin glycoprotein ligand-1) Other adhesion molecules CD44

33. Ⅲ. Functions
Participate in development and differentiation of immune cells
CD2----LFA-3
LFA-1----ICAM-1
Participate in development and maturation of thymocytes.

34. 2.Participate in immune response and regulation

36. 3. Participate in the adhesion of leukocyte and vascular endothelial cell during inflammation.

37. 4. Participate in lymphocyte homing
Lymphoid stem cell migrate to central lymphoid organs.
Mature lymphocyte migrate to peripheral lymphoid organs
Recirculation of lymphocytes
Lymphocyte migrate to the sites of inflammation

39. 5. Participate in regulation of apoptosis
Integrins inhibit cell apoptosis