1. Safety In the Microbiology Lab
Lab Safety: Everyone Is Responsible!
Safety In the Microbiology Lab
Rules and Symbols
Mr. Norazli Ghadin

2. Lesson objectives To know the general rules in microbiology lab
To know how to maintain aseptic condition
To know how to discard microbiological waste
To apply the rules when in the labs

3. Importance of rules Ensure safety of operator (YOUR SELF!!!!!!)
Safety of samples
Successful laboratory experimentation
Minimize hazards

4. Routes of infection
Oral
Skin
Conjunctiva
Lungs

5. General Safety Guidelines
Be Responsible at All Times.
Follow all instructions carefully.
No horseplay, practical jokes, pranks, etc.
Do not play with lab equipment until instructed to do so.
Food, drink, smoke and gum are not allowed in the microbiology lab .
Lab Safety: Everyone Is Responsible!

6. General Safety Guidelines
Don’t lick labels.
Don’t drink from laboratory glassware
Don’t wander about the laboratory; cause:
Accidents
Distract others
Promote contamination
Operate centrifuges, homogenizer and shakers safely
Immunize the laboratory workers

7. General Safety Guidelines
Keep the microbiology lab clean and organized.
Notify supervisor immediately of any accidents or unsafe conditions in the microbiology lab !
Wash your hands with soap and water after work.
Lab Safety: Everyone Is Responsible!

8. General Safety Guidelines
Listen to or read instructions carefully before attempting anything.
Wear safety goggles to protect your eyes from chemicals, heated materials, or things that might be able to shatter.
Notify your teacher if any spills occur.

9. General Safety Guidelines
Keep your hands away from your face.
Tie back long hair away from shoulders .
Wear glasses rather than contact lenses.
Never put into your mouth
Pencils
Pens
fingers etc
Clean up your lab area at the conclusion of the laboratory period.

10. General Safety Guidelines
Roll up loose sleeves.
Button-up your Lab Coat
Lab coat thickening.
Know the location of the fire extinguisher, Gas isolation switch, fire blanket, eyewash station, first aid kit & Exits.
Keep your work area uncluttered.

11. General Safety Guidelines
Take to the lab station what is necessary, Left outside
Coats
Jackets
Bags and books not required for the laboratory session.
Cover any open cuts on hands and other exposed skin surfaces

12. General Safety Guidelines
Wear mask N95 4 mycobacterium.
Do not wear any jewellery.
Carry out procedures to minimize risks of :
Spills
Splashes
Production of aerosols.
No slides or cultures are to be taken from, or brought into the lab without permission.

13. General Safety Guidelines
Don’t use your Mobile.
Medical devices
Chemicals
With gloves
RF interference
Do not attend calls with gloves.
Writing
Without gloves
Working area

14. General Safety Guidelines
Avoid wearing artificial nails
Keep natural nails <1/4 inch
Foot wear that completely covers the foot is required
No Open shoes
No rings or hand accessories
Do not wear loose clothing that could catch on fire
Do not open doors with glows

15. PERSONAL PROTECTIVE EQUIPMENT and PROCEDURES
Gowns
Safety Glasses and Eye Protection
Gloves
Mask
Hand washing
High Risk Individuals /Antenatal Considerations

16. Hand washing 16

17. Chemical Safety Do not inhale the fumes.
Never pour water into a concentrated acid.
Wash hands.
Wear goggles
Lab apron
Never taste any chemicals (never taste anything).

18. Electrical Safety
Lay electrical cords where no one can trip on them or get caught in them.
hands dry before using electrical equipment.
Never poke anything into electrical outlets.
Unplug cords by pulling the plug and not the cord.
Unplug all electrical equipment at the end of the lab period.

19. Heating Safety Let burners and hotplates cool down before touching.
Use tongs and/or protective gloves.
Never reach across an open flame.
Wait until the striker is in place before you turn on the gas.
Heating a test tube, move it around slowly.
Dry glassware should be heated.
Do not hold it in your hand
Never leave a burner or hotplate unattended.

20. Hypodermic syringes and needles
Common causes of occupational infections.
Collection
Processing
25%.
Avoid quick & unnecessary hand movements.
Examine for chips and cracks, and needles for barbs and plugs.
Use needle locking syringes.
Wear gloves.
Fill syringes carefully to minimize air bubbles and frothing.

21. Hypodermic syringes and needles
Expel air, liquid & bubbles vertically into a disinfected cotton swab.
Don’t forcefully expel into an open vial for mixing.
Mixing if the tip is held below the surface of the fluid.
Don’t manipulate by hand.
Place directly into a puncture-resistant container.
Decontaminate before
Disassembly
Reuse
Disposal.

22. CLEANING & Waste Disposal
Separate the non-infectious from the infectious waste.
Dispose the infectious waste to
Minimizes the risk to both staff and students
Facilitates the recycling of reusable material.

23. Sharps “Yellow" sharps container at the back of each laboratory.
Needles
scalpel blades
other sharp materials
Always move the "sharps container" to your work place to dispose of such items.
Do not wander around the laboratory carrying sharps.

24. Biogram Buckets
Containers of general purpose phenolic, hypochlorite (10 gm/L) laboratory disinfectant
Used for disposal of small contaminated items
used swabs
capillary tubes
wet slides
Pipettes
inoculated reagent strips
glass culture tubes.

25. Biogram Buckets Disposing pipettes, place tip first into the biogram
Not for Gram stains, non contaminated paper or matches.
1/40 dilution of the commercial product 18% phenol equivalent.
Change weekly; or if grossly soiled.

26. Biohazard Bin Centre of each laboratory.
For the disposal of contaminated waste, e.g.
Used culture plates
Contaminated paper towel.
Not to be used sharps & Any non contaminated paper wastes.
Do NOT use these bins for
Paper towel discarded after hand washing
Blotting paper discarded after blotting slides.
autoclaved before disposal.
Left open to allow penetration of the steam

27. Broken Glass
When any glassware is broken, notify the instructor immediately for assistance with disposal.
Non contaminated can be disposed of in the Billy cans
Contaminated should be placed into a stainless steel Billy and autoclaved prior to disposal in the glass bin.

28. Sink Don’t place any hazardous or infectious materials.
Don’t dispose of any solid material.

29. REPORTING OF INCIDENTS
All accidents and major spills should be documented on an "incident report form" through the relevant Departmental Occupational Health and Safety Officer
Copies of any documentation relating to an incident or safety hazard must be sent to both MSO and a copy should also be retained.

30. First Aid Injury: Burns
What To Do: Immediately flush with cold water until burning sensation is lessened. 30

31. First Aid Injury: Cuts, bruises What To Do:
Do not touch without gloves.
Pressing directly on minor cuts.
Apply cold compress to bruises to reduce swelling. 31

32. First Aid Injury: Fainting To Do:
Provide fresh air and have the person recline so that their head is lower than the rest of their body. 32

33. First Aid Injury: Eyes What To Do:
Flush eyes immediately with plenty of water for several minutes.
If a foreign object is lodged in the eye, do not allow the eye to be rubbed. 33

34. First Aid Injury: Poisoning
What To Do: Find out what substance was responsible for the poisoning and alert the teacher immediately. 34

35. First Aid Injury: Spills on the skin
What To Do: Flush with large quantities of water. For acid spills, apply baking soda solution. For base spills, apply vinegar or boric acid. 35

36. First Aid Injury: Electrical shock What To Do:
Shut off the current at the source.
Remove wire with rubber gloves.
Alert the teacher immediately. 36

37. What’s Wrong With This Picture?

38. What’s Wrong With This Picture?

39. What’s Wrong With This Picture?

40. What’s Wrong With This Picture?

41. When in doubt – ASK!!!
Do not carry out a new or unfamiliar procedure until you have been fully trained & understand the precautions necessary for safe working
DO NOT GUESS!!!!

42. HAZARDS SYMBOLS

43. Explosive
Chemical that will rapidly burn and explode

44. (Highly Flammable)
‘Flash point’ – temperature where vopour of a solution will burn if ignited.
Solution with ‘Flash point’ < 32’c

45. Oxidizing Substance Chemical that readily release Oxygen.
Contoh: HNO3, KNO3, NaNO3, AgNO3

46. Toxix Harmful chemical Cyanide and mercury nitrate – nerve, kidneyte
‘Iodine’ - lung and eye,
initiate ‘impetigo’
Formaldehyde (dalam Larutan 40% dipanggil ‘formalin’) – an irritant

47. (corrosive) All acid – burn tissue in contact.
NaOH and KOH – irritate, vomit and diarhea

48. (irretant & Harmful)
Cylohexance, etanol, Methanol, Ethyl acetate, Butanol and Potassium dichromate - dermatitis.
a dan b-Naphtol - skin irritant, damage kidney
Naphthylamine - anesthetic some drawbacks, irritation pada mucous membrane

49. Carcinogenic chemicals
carcinogens, mutagens, teratogens, respiratory sensitizers and substances with target organ toxicity.
Eg:
Ehtidium bromide, benzene

50. Environmentally hazards
These substances damage or pollute the environment.

51. Radiation hazards
Ionizing radiation
E.g: Uranium 235
Rhodium isotope

52. Biohazards Living organisms that can cause infection
bacteria, fungus, virus

53. Reference Food Standards Agency - Microbiological safety. html
Guidelines on Standard Operating Procedures for MICROBIOLOGY - Safety in Laboratories. tm WHO
Microbiology Rules for Using Disinfectants eHow com. htmL
Microbiology Safety and Staff Induction Manual University of Tasmania - Launceston Campus Version 2, February 2000
Mrs. Page Kennedy Middle School
Presented by: Leonard LaFazia