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William S. Klug Michael R. Cummings Charlotte A. Spencer Concepts of Genetics Eighth Edition Chapter 19 Recombinant DNA Technology Copyright © 2006 Pearson Prentice Hall, Inc.
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Recombinant DNA Technology Combines Several Experimental Techniques
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Recombinant DNA Technology Is the Foundation of Genome Analysis
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Restriction Enzymes Cut DNA at Specific Recognition Sequences
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Figure 19-1 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 19-2 Copyright © 2006 Pearson Prentice Hall, Inc.
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11,294 (There are at least this many known restriction enzymes)
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Figure 19-3 Copyright © 2006 Pearson Prentice Hall, Inc. palindrome
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Figure 19-4 Copyright © 2006 Pearson Prentice Hall, Inc. Not really a gap
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Vectors Carry DNA Molecules to Be Cloned Plasmid Vectors
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Figure 19-5 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 19-6 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 19-7 Copyright © 2006 Pearson Prentice Hall, Inc.
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Lambda ( ) Phage Vectors
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Figure 19-8 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 19-9 Copyright © 2006 Pearson Prentice Hall, Inc.
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Cosmid Vectors
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Figure 19-10 Copyright © 2006 Pearson Prentice Hall, Inc.
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Bacterial Artificial Chromosomes
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Figure 19-11 Copyright © 2006 Pearson Prentice Hall, Inc.
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Expression Vectors
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Figure 19-12 Copyright © 2006 Pearson Prentice Hall, Inc.
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DNA Was First Cloned in Prokaryotic Host Cells
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Figure 19-13 Copyright © 2006 Pearson Prentice Hall, Inc.
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Yeast Cells Are Used as Eukaryotic Hosts for Cloning
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Table 19-1 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 19-14 Copyright © 2006 Pearson Prentice Hall, Inc.
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Genes Can Be Transferred to Eukaryotic Cells Plant Cell Hosts
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Figure 19-15 Copyright © 2006 Pearson Prentice Hall, Inc. From Agrobacterium tumifaciens
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Mammalian Cell Hosts
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Figure 19-16 Copyright © 2006 Pearson Prentice Hall, Inc.
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The Polymerase Chain Reaction Makes DNA Copies Without Host Cells
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Figure 19-17 Copyright © 2006 Pearson Prentice Hall, Inc.
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The Polymerase Chain Reaction Makes DNA Copies Without Host Cells Limitations of PCR Other Applications of PCR
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Libraries Are Collections of Cloned Sequences Genomic Libraries Chromosome-Specific Libraries
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Figure 19-18 Copyright © 2006 Pearson Prentice Hall, Inc. Flow cytometry
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Figure 19-19 Copyright © 2006 Pearson Prentice Hall, Inc.
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Libraries Are Collections of Cloned Sequences cDNA Libraries
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Figure 19-20 Copyright © 2006 Pearson Prentice Hall, Inc.
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Specific Clones Can Be Recovered from a Library Probes Identify Specific Clones Screening a Library
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Figure 19-21 Copyright © 2006 Pearson Prentice Hall, Inc.
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Cloned Sequences Can Be Characterized in Several Ways Restriction Mapping
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Figure 19-22 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 19-23 Copyright © 2006 Pearson Prentice Hall, Inc.
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Nucleic Acid Blotting
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Figure 19-24 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 19-25 Copyright © 2006 Pearson Prentice Hall, Inc.
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DNA Sequencing Is the Ultimate Way to Characterize a Clone
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Figure 19-26 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 19-27 Copyright © 2006 Pearson Prentice Hall, Inc. T T C G T G A A 5’-TTCGTGAA…etc
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Figure 19-28 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 19-29 Copyright © 2006 Pearson Prentice Hall, Inc.
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DNA Sequencing and Genome Projects
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