1. Planaria Inquiry -do now 1.Are there specific drinks that companies market to young people to purchase? 2.How do you know? 1.Have any of you ever consumed these products?

2. What do these images say to you? What should we expect when consuming these products? What kind of ingredients are there in energy drinks?

3. Let’s think…. What kind of ingredients are there in energy drinks? list: What do those ingredients do to humans?

4. Energy Drink Nutrition Facts MONSTER ENERGY DRINK

5. Testing the Ingredients Is it better to test on humans or another organism? Why? Model Organism definition: a non-human species that is extensively studied to understand particular biological phenomena, with the expectation that discoveries made in the organism model will provide insight into the workings of other organisms such as humans Source: Wikipedia.org

6. Let’s Begin Let’s observe some planaria in petri dishes Watch their movement, observe using magnifying glasses As a group, decide what 4 solutions (some can be combinations or different concentrations of the same substance) that your group would like to test On your labs, record a “Hypothesis” that includes your “cause” and “effect”

7. Some important things to consider: – Each group will receive 3 planaria – You will need to record baseline data of each individual planaria in spring water over the course of 2 minutes. This is your control. All run times are 2 mins. – One at a time, place in a Petri dish. CAREFULLY flip over the closer petri dish so that the grid is on the bottom. – Count how many times the planaria crosses the line in 2 minutes. Class Discussion: What is our classroom standard unit for measurement of line crosses?

8. Other items: – The planaria should remain in the experimental solutions for 4 mins. prior to line-cross test. – Design your experimental protocol ALL experiments testing different chemical solutions need to have the same concentration (must also not exceed 40%). DEMO DILUTION – Be sure to detox planaria for four minutes in spring water between experiments – How should you collect data? Average?

9. Popular Drinks’ Nutrition Facts 9.5 fl. oz. Amt Per Serving Calories180 Total fat (g)3 Cholesterol (mg)5 Sodium (mg)95 Total Carbohydrates (g)33 Sugars (g)31 Protein (g)7 Calcium (%DV)20 Caffeine (mg)75 Calories140 Total fat (g)0 Sodium (mg)45 Total Carbohydrates (g)39 Sugars (g)39 Protein (g)0 Caffeine (mg)34 12 fl. oz. Amt Per Serving What about other drinks that you enjoy?