1. ANNUAL MEETING WITH OUR ADVISORY PANEL MEMBERS 12 January 2012 San Diego, California

2. STRATEGIC PLANNING TO MAXIMIZE IMPACT 12 January 2012 San Diego, California

3. Outline of Presentation  Project Overview  Morning Session  Afternoon Session

4. Project Overview

5. RosBREED Mission Statement We will develop and apply marker-assisted breeding, based on improved knowledge of industry value and consumer preferences, to accelerate and increase the efficiency of rosaceous cultivar release and successful cultivar adoption. 4 yrs $7.2M federal $7.2M matching Amy Iezzoni, Michigan State Univ. Cameron Peace, Wash. State Univ. This project is supported by the Specialty Crop Research Initiative of USDA’s National Institute of Food and Agriculture

6. RosBREED designed to bridge this chasm Genomics Resources Marker- assisted breeding Genomics knowledge More efficient development of new cultivars

7. Focus on fruit quality

8. RosBREED will utilize improved knowledge Courtesy David Byrne, Texas A&M Univ.) of industry values and consumer preferences to select target traits for Marker-Assisted Breeding (MAB) Impacts: increase new cultivar adoption enlarge market potential

9. RosBREED will develop tools Marker-assisted breeding in application For those traits that have value for producers, market intermediaries and consumers

10. Marker-Assisted Breeding Which parents to use? Which combinations to create? Which seedlings to progress? Which selections to trial? Which advanced selections to commercialize? Increased breeding efficiency

11. Long-term outcomes Four year project outcomes Increased gain in fruit quality per breeding cycle due to improved parent selection and improved mean progeny value More rapid availability of new cultivars with genetically superior fruit quality Improved profitability and sustainability of US rosaceous fruit, nut, and floral crops with increased consumption and enjoyment Implementation of MAB by breeding programs RosBREED’s Target Outcomes

12. Project Assistants Joan Schneider Audrey Sebolt Extension Team Leader: Cholani Weebadde Genomics Team Leader: Dorrie Main Genotyping Team Leader: Nahla Bassil BIMS Team Leader: Gennaro Fazio Socio- Economics Team Leader: Chengyan Yue MAB Pipeline Team Leader Cameron Peace Pedigree- Based Analysis Team Leader: Eric van de Weg Breeding Team Leader Jim Luby Apple  Susan Brown  Kate Evans  Jim Luby Strawberry  Jim Hancock  Chad Finn  Tom Davis Peach  John Clark  Dave Byrne  Ksenija Gasic  Tom Gradziel Cherry  Nnadozie Oraguzie  Amy Iezzoni Executive Committee Cameron Peace Nahla Bassil Gennaro Fazio Jim Luby Dorrie Main Jim McFerson Eric van de Weg Cholani Weebadde Chengyan Yue Project Director Amy Iezzoni RosBREED Organization

13. Demonstration Breeders & Breeding Trainees

14. Morning Session Goal = provide information to AP members to set the stage for receiving advice

15. StartEnd Audience/ Group Presenter/moderatorTitle 8:008:30AllAmy IezzoniIntroductions 8:309:00AllAmy IezzoniStrategic planning to maximize impact 9:0010:00All Karina Gallardo Dorrie Main Nahla Bassil Eric van de Weg Gennaro Fazio, Jim Luby Trait Targets, Genome Scans, Pedigree- Based Breeding Analysis and Toolbox (Obj. 1-3): Goals, Deliverables, Challenges, Year 3 Deliverables, and Q & A 10:0010:30 Break (computer demonstrations) 10:3011:00All Cameron Peace Cholani Weebadde Marker-Assisted Breeding Pipeline and Extension (Obj. 4-5: Goals, Deliverables, Challenges, Year 3 Deliverables, and Q & A 11:0011:15AllMichael CoeEvaluation of extension efforts 11:1511:45AllKarina Gallardo Comparing the importance of fruit traits to different stakeholders 11:451:00 Lunch Morning session

16. Major deliverables in Year 2 Enabling computer-based accomplishments that allow us to find, polish and put the Jewels into application Computer demonstrations at breaks: Gennaro Fazio Toolbox Eric van de Weg PBA Software Umesh Rosyara PBA Software Dorrie Main GBrowse Cholani Weebadde Videos

17. External Evaluation of Extension – Dr. Michael Coe

18. Comparing the importance of fruit traits to different stakeholders: preliminary socio-economics survey results

19. Analogy of Jewels in the Genome Find An individual’s genome is the full complement of genetic information that it inherited from its parents. Within this vast repertoire of genetic information, individuals genes are being discovered that control critical production and fruit quality traits. Put into application in breeding Jewels = valuable genetic discoveries that are put into breeding application Polish

20. Examples of Jewels

21. RosBREED seeks to find, polish and put into application Marker- Assisted Breeding for valuable Jewels in the Genome Increase breeding efficiency and enhanced acceptance of new cultivars desired by industry and consumers

22. The Jewels are linked on chromosomes 56 chromosomes in strawberry!!

23. Afternoon Session Goal = Receive advice from AP members

24. 1:001:30 Discussion session 1 Group 1Industry opportunities, needs and limitations 1:001:30 Discussion session 1 Group 2Breeding opportunities, needs and limitations 1:302:15AllDS1 - Groups 1 & 2Reports & Discussion 2:153:00 Discussion session 2 Group 1 Ideas and approaches for addressing industry opportunities, needs and limitations 2:153:00 Discussion session 2 Group 2 Ideas and approaches for addressing breeding opportunities, needs and limitations 3:003:30 Break (computer demonstrations) 3:304:00AllDS2 - Groups 1 & 2Reports & Discussion 4:005:00AllCameron PeaceRound table 5:00AllDinner on your own or in working groups Afternoon Session

25. Cook Harrison van Sickle Hirst Watkins Karp Abbott Buell Gardiner Hummer Cheng Simon Ross AP Color Key Industry Extension Scientific Allen Carr Dodd Grim Korson Goldberger Olmstead Sherman Arus Avari Bierlink Curtis Perry Sparks Seavert Bliss Baugher Stokes Moffitt Advisory Panel Members

26. RosBREED’s International Partners Plant Research Intl., The Netherlands East Malling Research, United Kingdom INRA – Bordeaux, Avignon & Angers, France CRA-FRU Rome, IASMA, Italy Andres Bello University, Chile ARC, South Africa Plant & Food Research, New Zealand

27. Strategic planning to maximize impact We are midway through RosBREED... What adjustments need to be made? A possible second project?

28. Discussion session 1 1:001:30 Discussion session 1 Group 1Industry opportunities, needs and limitations 1:001:30 Discussion session 1 Group 2Breeding opportunities, needs and limitations 1:302:15AllDS1 - Groups 1 & 2Reports & Discussion 1.As we are mid-way through RosBREED, how are we doing meeting the currently defined industry and breeding opportunities, needs, and limitations? 2.Are there unanticipated opportunities, needs and limitations that we should address within the current project? 3.Thinking ahead to a possible second project, are there opportunities, needs and limitations that need to be addressed?

29. Discussion session 2 2:153:00 Discussion session 2 Group 1 Ideas and approaches for addressing industry opportunities, needs and limitations 2:153:00 Discussion session 2 Group 2 Ideas and approaches for addressing breeding opportunities, needs and limitations 3:003:30 Break (computer demonstrations) 3:304:00AllDS2 - Groups 1 & 2Reports & Discussion 1.Are there methods or plans of work that could be done differently to better meet RosBREED’s current industry and breeding project goals? 2.What are the ideas and approaches for addressing any unanticipated opportunities, needs and limitations within the current project? 3.What are the ideas and approaches for addressing any future industry and breeding opportunities, needs and limitations?

30. Acknowledgements This project is supported by the Specialty Crop Research Initiative of USDA’s National Institute of Food and Agriculture

31. MSU Amy Iezzoni (PD) Jim Hancock Dechun Wang Cholani Weebadde WSU Cameron Peace Dorrie Main Kate Evans Karina Gallardo Vicki McCracken Nnadozie Oraguzie Former WSU Raymond Jussaume Mykel Taylor Cornell Susan Brown Kenong Xu Clemson Ksenija Gasic Gregory Reighard Texas A&M Dave Byrne Univ. of CA-Davis Tom Gradziel Carlos Crisosto Univ. of New Hamp. Tom Davis Univ. of Minnesota Jim Luby Chengyan Yue Oregon State Univ. Alexandra Stone Plant Research Intl, Netherlands Eric van de Weg Marco Bink USDA-ARS Nahla Bassil Gennaro Fazio Chad Finn Univ. of Arkansas John Clark