1. Engineering of E.coli for production of poly(3-hydroxypropionate)
Tallyne Vasconcelos

2. What are polyhydroxyalkanoates (PHAs)?
biodegradable and biocompatible thermoplastics used in several applications, such as medicine, pharmacy and agriculture
the production was a way of using the glycerol in excess originated from the biodiesel industry
poly(3-hydroxypropionate) was the type of PHA used in this experiment

3. Why to use E.coli?
E.coli strains are widely used as hosts for microbial production of valuable compounds, like biofuels, chemicals and polymers
It is highly adaptable and widely know genetic and metabolically

4. The process

5. Results

6. Results

7. Conclusion
the microbial P3HP production from glycerol was improved greatly by constructing a genetically stable E.coli recombinant strain
To overcome the instability due to plasmid loss, two strategies were tested and combined
Better results were obtained: the recombinant strain Q1738 produced g/L of P3HP from glycerol in aerobic fermentation

8. Thank you!