1. 2005 Plant Propagation Workshop Paul A. Thomas and Bodie. V. Pennisi The University of Georgia Southeast Greenhouse Conference Producing Plants From Seed

2. No Two Seeds Are Alike

3. A seed’s origin, in terms of climate and geographical location. This can Provenance : A seed’s origin, in terms of climate and geographical location. This can Have profound effects on seed germination and the plant’s survival. Example: Hemlocks grown from southern North Carolina seed sources are more heat tolerant than Hemlocks grown from Pennsylvania seed sources. Seed Collecting

4. Seeds collected from hybrids rarely look like the parent plant due to the random reassortment of genetic material, and the random sources of pollen. Native species tend to be more stable, but also have variation between generations. To get a clone, try vegetative propagation!

5. – Populations of plants Landraces – Populations of plants maintained over hundreds of years by farmers by holding over some of the seed crop. This allowed for local specialization and great differences in genetic makeup Terms To Know

6. Heritage Varieties Vegetables and flowers that were grown and passed on through many generations by families.

7. – Progeny from identical Inbred Lines – Progeny from identical lines that are homozygous. These are then used to make hybrids – The progeny of two Hybrid Lines – The progeny of two or more inbred lines Terms To Know

8. Seed Cleaning / Separation For many dry seed, simply crush dried material and blow gently, transferring the seed from hand to hand. Cleaning seed reduces disease and weed seed from growing along with your selection

9. Fleshy Berry Seed

10. Fermentation Fermentation Flotation Flotation Blender Separation Blender Separation Screen-Press Separation Screen-Press Separation Gravity Separators Gravity Separators Hand Separation Hand Separation Sifting Sifting Drying (Heating Pine Cones) Drying (Heating Pine Cones) Extraction Methods

11. Seed Deterioration Seeds lose half their storage life for every 1% increase in seed moisture between 5 and 14%. Seeds lose half their storage life for every 5 degrees C increase in storage temperature between 0 o and 50 o C.

12. Seed Storage Moist Storage @35 to 50 o F Dry Storage @ 35 to 50 o F* Cold Storage @ 0 o F, –18 o C * Cryopreservation @ -196 o C * *** at 3 – 8% Moisture *** at 3 – 8% Moisture

13. – These seeds are able Recalcitrant Seed – These seeds are able to germinate without dessicating. These seeds lose viability after drying and must be planted quickly. be planted quickly. Oak, Maple, Coffee – The seeds dessicate Orthodox Seed – The seeds dessicate after reaching full development to allow the seed to be quiescent or dormant until conditions are right to germinate. conditions are right to germinate. Beans Terms To Know

14. SEED STORAGE Recalcitrant Seed – Short-term Viability – Store warm and moist (ASAP) Tropical – Store warm and moist (ASAP) Coffee, Cocoa, Mango, Macadamia, Avocado, Tea – Store cool and moist (ASAP) Subtropical – Store cool and moist (ASAP) Maple, Oak, Elm, Poplar, Salix,

15. Orthodox Seed – Store dry and cold (Under 1 yr) Short-Lived – Store dry and cold (Under 1 yr) Vinca, Pansy, Begonia - Store dry and cold ( 2-5 years) Medium - Lived - Store dry and cold ( 2-5 years) Marigold, Petunia, Coleus – Store dry and cold ( 5-200 years) Long - Lived – Store dry and cold ( 5-200 years) Morning Glory, Zinnia, Hollyhock

16. Life Expectancy Of Selected Seeds Sugar Maple 2 weeks English Elm 26 weeks Cocklebur 16 years White Clover 90 years Sensitive Plant 200 years Indian Lotus 1,040 years Artic Lupine 10,000 years

17. Testing Viability - Simplified

18. Handling Tiny Seeds Mix Seed with Sand

19. Fungicides/RhyzobiaFungicides/Rhyzobia PolycoatingPolycoating Pre-germinatedPre-germinated Seed Coatings

20. Expiration Date Planting Depth Directions Important Information Source

21. GerminationPercentage Lot Number Scientific Name More Information

22. The Cycle of Life

23. 1. Imbibed water stimulates Gibberellin synthesis. 2-3. Gibberellins diffuse to the aleurone layer and stimulate the synthesis of enzymes.. 4-5. Enzymes break down the starch and the sugars are transported to the developing embryo.

24. Seed and Plant Dormancy Dormancy is the condition In which seeds will not Germinate… even when most of the environmental conditions are permissive for germination. There are many types of dormancy!

25. Advantages of Seed Dormancy Favors seedling survival Favors seedling survival Creates a seed bank Creates a seed bank Seed dispersal (birds) Seed dispersal (birds) Synchronizes germination with seasons Synchronizes germination with seasons

26. Types of Dormancy in Seed – The seeds are able to Germinate upon Quiescent – The seeds are able to Germinate upon imbibition of water at permissive temperatures. – Seeds cannot germinate Primary Dormancy – Seeds cannot germinate even if immediate conditions are right. This form of dormancy delays germination until season, or other macro-environmental issues are right for survival. – An additional level of Secondary Dormancy – An additional level of Protection to prevent germination. Can be induced under very unfavorable conditions such as drought or cold, etc.

27. - Imposed by Exogenous Dormancy - Imposed by factors outside the embryo. factors outside the embryo. Seed coat. – Imposed by Endogenous Dormancy – Imposed by factors within the embryo. Underdeveloped embryo. Types of Dormancy in Seed

28. - Factors outside Secondary Dormancy - Factors outside the seed induce dormancy after the seed was previously non-dormant. seed was previously non-dormant. High (Thermo-dormancy) Soil Temperatures (Thermo-dormancy) Types of Dormancy in Seed - Any combination Double Dormancy - Any combination of endogenous and exogenous factors

29. Physical – Impermeable seed coat : Scarification Mechanical – Seed covering restricts radical : Removal Chemical – Inhibitors in seed coat : Removal / Leaching Exogenous Dormancy

30. Morphological - Underdeveloped embryo : Warm Stratification Physiological Non-Deep – After Ripening : Dry storage Photo-dormant : Exposure to red light. Intermediate – Embryo/coat separation : Cold Stratification Epicotyl – Epicotyl dormant : Warm - Cold Stratification Endogenous Dormancy

31. Morpho-physiological – Some combination of underdeveloped embryo and physiological dormancy: Cycles of warm and cold stratification. Exo-Endodormancy – Combination of exogenous and endogenous dormancy conditions Sequential combinations of dormancy releasing treatments, Eg. Scarification followed by cold stratification Double Dormancy

32. Thermo-dormancy - High temperatures induce dormancy Growth regulators or Cold stratification Conditional – Change in ability to germinate is related to time of year. Chilling or Warm stratification Secondary Dormancy

33. Photodormancy : A type of dormancy Photodormancy : A type of dormancy where the ability of the seed to germinate is controlled by the wavelengths and durations of light received by the embryo. Lettuce, Butterflyweed, Tobacco

34. Germination Of Lettuce

35. Preconditioning Seeds (for more uniform germination) Mechanical scarification Soaking In Water Acid Scarification Moist Chilling / Freezing Double Dormancy Methods:

36. Mechanical Scarification

37. Acid Scarification An alternative to scraping the seed coat is to use acid to etch through the coat. There are many reference books that advise which acid and how long to treat.

38. Hot Water Scarification Water temperature should be over 110 O F Let soak for a few hours. Stir often. Do not re-heat the water. Plant ASAP.

39. Moist Stratification Cold or Warm

40. Seed Soaking / Leaching Seed soaking optimizes the amount of imbibed seeds and evens out the stage of imbibition by insuring 100% moisture availability Soaking too long can cause anoxia and reduced germination. 12-36 hours is a very common soaking period.

41. Clean & Organized Environment

42. Window-Box Propagation Two clear halves of sweater/storage boxes taped together with duct tape as a hinge make ideal propagation boxes! Place the box in a sunny window, and open it up for an hour at night to replace air and dry off leaves. Works for almost all tropical plants.

43. Root Zone Heating Rooting medium temperature, given control, may be applied to speed up growth. A good example is using heating pads under planted seed flats to speed up germination or rooting. The right temperature can take a week or month off of rooting schedule and can take a few days to a few weeks off of germination. Time is money.

44. Electric Heating Tapes

45. We need to provide seedlings a film of water to reduce evaporation, and the loss of water. Too much water is as bad as too little water! Mist Benches

46. Fungicides BanRot! Follow The Label Directions!

47. Seedling Production Options Community Pot Seeding Box Plug Tray Fabric Matt Hydroseeding Direct Broadcast

48. Proper seed bedProper seed bed Proper planting seasonProper planting season Proper pretreatmentProper pretreatment Proper seed handlingProper seed handling Proper sowing depthProper sowing depth Proper sowing rateProper sowing rate Proper post-sowing careProper post-sowing care Sowing Success

49. Minimize root disruption! Never press on roots or handle roots! Never compact soil around roots Work quickly and gently Re - water in transplants in minutes Transplanting Seedlings

50. Use low levels of fertilizer 50 ppm! Do not allow to become dry. Do not allow to stay saturated! Modify light levels according to species. Scout seedling several times a day! Growing Out Seedlings

51. Some of the seeds of hope Planted tentatively in the fall Have not come up They lie stillborn and unrealized Somewhere in the spring soil Decaying The Strongest and best ones Pushed up though the leaves And layers of cold, hard resistance Right into clear blue air And stand there nakedly green Breathing Some of the seeds of hope Planted tentatively in the fall Have not come up They lie stillborn and unrealized Somewhere in the spring soil Decaying The Strongest and best ones Pushed up though the leaves And layers of cold, hard resistance Right into clear blue air And stand there nakedly green Breathing It's always that way with growing things Never knowing at the start Which will make it and which will fail But the thing to hold fast to Never to lose faith in, is simply, Sowing Sowing Gardener by Ann North