1. Clean Seas Tuna R & D Programme - Some early outcomes Mike Thomson – R & D Manager CRC Planning & Reporting Forum, Adelaide 12-13.May.09

2. CST will engage a full time R&D Manager who will be responsible for all aspects of the nine core activities Establishment of R & D Capacity

3. The R & D Manager will establish and enhance cost effective collaborative links between research providers (inside and outside of the CRC) via effective relationships (e.g. CRC partners) R & D Capacity - Collaborators

4. The R & D Manager will establish and enhance cost effective collaborative links between research providers (inside and outside of the CRC) via effective relationships –e.g. Kinki University MOU 22.Sep.2008 R & D Capacity - International

5. AND Ausindustry, PIRSA, the South Australian Marine Finfish Farmers Association, Northern Territory Department of Regional Development / Primary Industry / Fisheries and Resources, Panaquatic and Skretting AND Kinki University, Hellenic Center for Marine Research, Heinrich-Heine Universität, University of Maryland, EU research consortiums SELFDOTT and REPRODOTT, Inter American Tropical Tuna Commission and the Tuna Research and Conservation Center

6. R & D Capacity - Post Graduates

7. The R&D Manager will provide annual progress reports to the Seafood CRC that detail achievements against the agreed milestones for each of the nine Core Activities R & D Capacity - Reporting !

8. SBT 1. To produce quality fertilised SBT eggs for use in larval rearing, weaning trials and for commercial production of juveniles –Developed anaesthetics and handling techniques (needs improvement) –Development and refinement of hormones and delivery systems (successful !) –Initial data on levels of reproductive hormones (testosterone, 11-ketotestosterone (11KT) and 17β-estradiol (E2) in SBT have been collected –An ELISA technique for quantifying circulating blood levels of LHRH has been tested successfully in Australia for SBT –Viability of thawed samples of cryopreserved SBT sperm has been demonstrated Results versus Objectives

9. SBT 1. To produce quality fertilised SBT eggs for use in larval rearing, weaning trials and for commercial production of juveniles –SBT and YTK KiSS receptor cDNA sequences have been isolated –Ligation of SBT KiSS receptor into an expression vector is ongoing –Ultimate aim of developing an optimised hormone delivery system based on KiSS for advancing SBT and YTK puberty Results versus Objectives

10. SBT 1. To produce quality fertilised SBT eggs for use in larval rearing, weaning trials and for commercial production of juveniles –World first spawning March.2008 –Continuous spawning over a 35 day period from 12.March.2009 –Over 50 million eggs fertilised –Numerous instances of over 90% fertilisation achieved Results versus Objectives

11. SBT Egg Development

12. SBT 2. To establish protocols and methods, including feeds/feeding for the commercial production of juveniles to support a propagated tuna aquaculture industry. –30 million larvae produced –http://www.youtube.com/watch?v=J8c NmdBL73Qhttp://www.youtube.com/watch?v=J8c NmdBL73Q –Acceptance of rotifer, artemia and live fish larvae –SBT fingerling production, with fish having achieved approximately 8cm in length and >45 days in age –Progress in weaning onto moist pellets Results versus Objectives

13. Weaning onto moist pellets

14. SBT Larval Research Commercial protocols at CST Feeding protocols being developed at SARDI Eggs ozonated at CST, translocated and early larval work conducted at NSW DPI Tanks and copepods prepared at Darwin Archival health samples to be analysed at SARDI and inter- state laboratories (PCR and tissue culture) Egg biochemistry taken for TAFI

15. SBT 3. To develop / evaluate a cost effective diet for growout of wild caught and propagated SBT with FCR < 4.5 : 1.0 –2 trials conducted with Ridleys in 2008 season (CRC funded) –Follow up trials with Ridleys and Skretting in 2009 (pre- commercial) –Benchmarking relationships developed in Japan with Kinki and Skretting Results versus Objectives

16. YTK Reduce deformity rates to less than ten percent before hatchery grading –TAFI project Egg ozonation tested safe and implemented (for SBT) Egg quality confirmed as OK Live feed quality checked and improvements made Physical parameters checked and improvements made Malformation levels reduced over time (from 28 to 50% to 12 to 29%) and tanks modified Training provided in key areas: malformations, ozonation and rigour in measurements Results versus Objectives Recommendations given and most implemented and or incorporated into new research

17. YTK Reduce deformity rates to less than ten percent before hatchery grading –Current larval project Start with high rotifer densities (15-20/ml) and also artemia densities (25/ml) and maintain Artemia from 12 DPH (or as early as 10) and no need for co-feeding of rotifers past 3 days and test inert diet from day 8 Results versus Objectives

18. YTK Reduce production costs through improved fish health management and disease control Testing of potential in feed medicines Results versus Objectives

19. YTK Reduce production costs through improved fish health management and disease control Fluke prevalence against treatment Results versus Objectives

20. YTK Reduce production costs through improved fish health management and disease control Salinity trial (small fish) Statistics pending Results versus Objectives

21. YTK Reduce production costs through improved fish health management and disease control In feed cage trial data to be analysed Anti-fouling trials to be established Blood fluke and kudoa surveys to be initiated Health protocols for SBT larvae to be developed Testing of latest winter diets for performance Results versus Objectives

22. YTK Improve growth and FCR with better feeds and regimes –Concept proposal accepted by CRC RAC (within TBP) –Full proposal to next mtg –PhD started Results versus Objectives

23. YTK Improve grow out processes to reduce cycle time –“Understanding” YTK project first mtg Wednesday ! Effects of stocking size and sea temperatures Effects of maturity Describe baseline product quality parameters (flesh oil level and distribution), nutritional composition, shelf life and assess effects of new product formats and finishing diets Results versus Objectives

24. Develop a range of genetic improvement options for YTK and SBT - Genetics scoping study CST can avoid excessive inbreeding by deploying a. more tanks, b. DNA tagging c. crossbreeding (cost $5K to $50K) = the major initial outcome. 13 polymorphic loci have been verified which can be used to tag animals and identify population structure (stocks across WA, SA, Victoria, NSW, & NZ) We now have people and tools on the ground that can be used to manage stocks, control inbreeding, etc. CST should consider, over time, a realistic aim to develop an optimised cost-effective breeding program utilising the maximum number of families appropriate to the prevailing business environment Draft final report submitted and the company is considering future options including mentoring project and wider collaboration (NIWA) Results versus Objectives

25. Establish a post harvest capability to take advantage of market driven cost effective value adding opportunities Report on “European Market Development Opportunities” Short study on effects of maturation and harvest practices on product quality (report pending) Proposal recently submitted to conduct: –consumer preferences for species, packaging (MAP and VAC), product formats and pricing –Market research and plan and detailed trade research report in US and Europe –Investigate shelf life extension methods including MAP –Development and testing of new fresh and frozen product format Results versus Objectives

26. Visiting scientist – Dr. Clive Talbot Industry bursary – Morten Deichmann (NIWA) Travel grants – various conferences and hatchery tours in 2009 Marine Fish “Hub” project Generally very happy to be a major contributory partner within the Seafood CRC ! Other Initiatives

27. “ This work formed part of a project of the Australian Seafood Cooperative Research Centre, and received funds from the Australian Government’s CRCs Programme, the Fisheries R&D Corporation and other CRC Participants”. AND Ausindustry, PIRSA, the South Australian Marine Finfish Farmers Association, Northern Territory Department of Regional Development / Primary Industry / Fisheries and Resources, Panaquatic and Skretting AND Kinki University, Hellenic Center for Marine Research, Heinrich-Heine Universität, University of Maryland, EU research consortiums SELFDOTT and REPRODOTT, Inter American Tropical Tuna Commission and the Tuna Research and Conservation Center