1. Nanotech in Biomedicine: Biodegradable Polymer Nanoparticles for drug delivery through mucosal Membranes
By Group #3
Phillip Keller
Micheal Jones
Krista Melish
James Kancewick

2. Background and Introduction Problem and Basic Principles
Overview
Background and Introduction
Nanotech in Biomedicine
Problem and Basic Principles
Nano Particle Immobility/Transport Process
Experiment and Results
Polymer Prep/Transport Characteristics
Improvements and Future Research

3. Nanotechnology Introduction Very Small Very Powerful Many Applications
Rapid Research Growth

4. Introduction: Biomedicine
Drug delivery
Biodiagnostics
Biosensors
Bioimaging

5. Drug Delivery Selective Targeting Protects Deliverable
Organs
Pathogens
Cancer Cells
Protects Deliverable
Less Medicine Wasted
Controllable Release

6. Biodiagnostic Detection of Nucleic Acids Detection of Proteins
PCR (Polymerase chain reaction)
Detection of Proteins
High levels of certain proteins are indicative of various cancers and diseases
AB_Still_z_0022_prv.jpg

7. Biosensors

8. Bioimaging Enhance Current Imaging Techniques Compliment MRI/CT
Provide contrast in previously unobtainable areas
Reduce need for exploratory surgery

9. Problems with Nano Particle Drug Delivery
100X Salival Mucus

10. Cervicovaginal Mucus Fibers
Mucus Penetration
Cervicovaginal Mucus Fibers
Cystic Fibrosis
Thickening of lung mucosal layers
Eye
Gut
Drug delivery to impede cancer growth
Female Reproductive Diseases

11. Mucus Penetration

12. Particle Biodegradability and Drug Delivery
No Interaction with Mucus Fibers
Dense PEGylation of particle surface allows mucus penetration due to neutral charge distribution

13. Biodegradable Nanoparticle With Encapsulated Drug

14. Synthesis of PSA-PEG One Step Formulation Process Diblock Copolymers
Covalently Bonded
The PSA is hydrophobic because ____ . It is the one in the red box- sebacic acid
Synthesized by melt polycondensation

15. Mucus-Penetrating Nanoparticle
Dense Surface Coating of PEG
Low Molecular Weight
Neutral Surface Charge
Non-Penetrating particles have highly negative surface charge

16. Flexible Drug Delivery
Controlled Drug Release
Vary ratio of PEG to PSA to alter particle degradation rate

17. Physicochemical Properties of Nanoparticles
Zai Potential
Surface charge characteristic
Dw/Dm
Ratio of dispersion coefficients for particle in water compared to human cervical-vaginal mucus

18. Results & Conclusions
Rapid Transport
Can Penetrate CVM Rapidly

19. Penetration in Cystic Fibrosis Sputum
Rapid Penetration of CF Sputum

20. Improvements and Further Research
Increase Particle Transfer Rates
Adapt to more specific tissues

21. Improvements and Further Research
Develop Applications for a Wider Range of Diseases
Create nanoparticles capable of accepting a wider array of drugs
Develop more chemically gentle manufacturing processes for coating particles in PEG

22. Improvements and Further Research
Reduce Cost of Production
Make economically viable
Increased targeting reduces dosage and drug waste

23. Questions?

24. Resources
Tang, Benjamin, Michelle Dawson, Samuel Lai, Ying-Ying Wang, Jung Suk, and Ming Yang. "Biodegradable polymer nanoparticles that rapidly penetrate the human mucus barrier." Proceedings of the National Academy of Sciences (2009):
Various websites listed in slides

25. Micheal Jones Phillip Keller Krista Melish James Kancewick
Rebuttal From Group U3
Micheal Jones
Phillip Keller
Krista Melish
James Kancewick
media.podcasts.ox.ac.uk/mat/nanotechnology/nanotechnology_albumcover.jpg

26. What we did well Confidence Graphics Well Dressed Basic Intro
More in depth article

27. Areas For Improvement Elimination of filler words
Increasing presentation time and content
Better eye contact with the audience
Relabeling graphics with small words
Increase group understanding of the article

28. Biodegradable Polymer Nanoparticles for drug delivery through mucosal Membranes Group U1
The overall presentation was very good and interactive with the audience.
The nanoparticle PSA-PEG showed to be a very effective polymer since it has good diffusion through some specific organic membranes
Further research can be done on improving the efficiency in delivering drugs
Its still a problem to power this particles.
We still have to do work on targeting the right receptors because in body we may have several similar mucosal membranes with similar biological and chemical properties that are healthy.

29. Biodegradable Polymer Nanoparticles
Review of Group U3’s Presentation-
Biodegradable Polymer Nanoparticles
for Drug Delivery through Mucosal Membranes
By Group U2:
-Kyle Demel
-Keaton Hamm
-Bryan Holekamp
-Rachael Houk
images/nanoparticles.jpg

30. The presenters could have improved in the following areas:
Presentation too short:
30-minute presentation only lasted 15
Needs elaboration on further research:
Delivery with viral protein coatings
Transfer through cellular membranes
Explain the pictures on the slides; do not just add pretty pictures because you have to
Oral delivery
Practice speech
Several group members were not knowledgeable on article
Excessive use of filler words
Work on timing and transitions

31. Nanotech in Biomedicine: Biodegradable Polymer Nanoparticles for drug delivery through mucosal Membranes
Reviewed by Group U4

32. Presentation/Oral Review
Overall, the speakers sounded very confident, thus enabling the audience to stay attentive to the information presented as opposed to the flaws in speech.
Excellent slides with a good balance of text and graphics. Both were large enough for the audience in the back to see clearly
Nice hands-on example with the grapefruit/lemons for further explanation of the topic.

33. Technical Review
Perhaps this is because this research is so new, but we would have liked to have a more in depth description on how the mucus penetration would work in a variety of applications.
Also, maybe devote a little time to the future of this technology(different diseases it could fight, and different barriers the nanoparticle could help break through other than the mucus)
Overall however, good presentation that left us with few question. The questions we did have were answered well.

34. Nanotech in Biomedicine : Biodegradable Polymer Nanoparticles for drug delivery through mucosal Membranes
Review of Team U3 by Team U5 – Jaynesh Shah, Greg Pudewell, Edwin L. Youmsi Pete and John Pack. 34

35. Oral and Quality of Slides Review
Speakers did a good job in the oral aspect of the presentation. They were loud and clear confident. Implying they had done a good amount of research on the topic.
The slides clear, visible and not overcrowded. Team U5 made good use of several pictures, images and graphs. 35

36. Technical Review
The presentation was satisfying from a technical standpoint.
We would have however liked them to discuss the use of biosensors in a little more details with respect to the issue of drug delivery 36

37. Nanotech in Biomedicine: Biodegradable Polymer Nanoparticles for drug delivery through mucosal Membranes
Presented By Group #3
Phillip Keller
Micheal Jones
Krista Melish
James Kancewick
Critiqued by: Group U6 - Pavitra Timbalia, Michael Trevathan, Jared Walker

38. Review – Slides and Oral Presentation
The slides were well balanced with graphics and text.
All presenters were well dressed and maintained an appropriate tone.
The demonstration involving the fruit to
describe the receptors of drugs helped to
open the presentation and get the audiences attention.
A few of the graphs were difficult to read and understand, and were not explained carefully.
The evolution leading to the development
of PEG wasn’t explained until someone asked about it during the questions section.

39. Review - Technical
This presentation dealt with the delivery of drugs through the use of nano-particles engineered specifically to be able to penetrate through thick mucus that was previously not possible.
Future research needs to be done to test the particles ability to deliver drugs
The tests done for the paper only showed
that the particles were able to penetrate the
mucus - they didn’t contain any drug payload.
This technology could help to treat many diseases such as cystic fibrosis, provided future research is done to reduce the cost of production.

40. Review for U3 from G6
Jung Hwan Woo

41. What are the factors that affects these rates?
The table on the top left shows the diffusivity of the nanoparticle in mucus compared to the diffusivity in water. It is used to indicate the rate at which the nanoparticle travels in the body through mucus.
What are the factors that affects these rates?
What can be done to improve the particle transfer rate as suggested on the bottom left?
Jung Hwan Woo