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Lecture 15 Tuesday 3/4/08 Enzymes Michealis-Menten Kinetics Lineweaver-Burk Plot Enzyme Inhibition.

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Presentation on theme: "Lecture 15 Tuesday 3/4/08 Enzymes Michealis-Menten Kinetics Lineweaver-Burk Plot Enzyme Inhibition."— Presentation transcript:

1 Lecture 15 Tuesday 3/4/08 Enzymes Michealis-Menten Kinetics Lineweaver-Burk Plot Enzyme Inhibition

2 Last time 2/21/08

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7 V max =k cat * E t Turnover Number: k cat Number of substrate molecules (moles) converted to product in a given time (s) on a single enzyme molecule (molecules/molecule/time) H 2 O 2 + E  H 2 O + O + E 40,000,000 molecules of H 2 O 2 converted to product per second on a single enzyme molecule.

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22 Enzymes, Inhibition S + E → E + P 1) Mechanism and active intermediate (E·S) 2) Rates 3) Net Rates

23 4) Pseudo Steady-State

24 (Michaelis-Menten plot) V max -r s S 1/2 k m =S 1/2 therefore k m is the concentration at which the rate is half the maximum rate

25 Competitive Inhibition E + S E·S E + P + I E · I 1)Mechanisms 2)Rates k1k1 k2k2 k3k3 k4k4 k5k5

26 From before (no competition): Intercept does not change, slope increases as inhibitor concentration increases No competition Competition

27 Uncompetitive Inhibition E + S E·S P E · S· I I k1k1 k2k2 k3k3 k4k4 k5k5


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