1. PCR Overview Lifted from various powerpoint presentations on the internet

2. PCR (Polymerase Chain Reaction) Invented by Dr. Kary Mullis in 1983 In vitro amplification of specific DNA fragment Uses two oligonucleotide primers complementary to opposite strands of a duplex DNA. Taq DNA polymerase –Thermus aquaticus Thermostable polymerases

3. Invented by Kary Mullis Mullis and Faloona, 1987. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Nobel Prize 1993

8. PCR DNA replication of a desired region of the genome in a test tube.

14. Templates for PCR

15. Determine your target region

18. PCR Animation

19. PCR strip tubes

20. Thermal Cycler

21. Gel Electrophoresis