1. Bioinformatics On Genomics Hsueh-Fen Yuki Juan April 28, 2003

2. Outline Gene Sequence Searching Cutting Site for a Specific Sequence Sequence Alignment Search for Conserved Domains ORF Finding

3. Gene Sequence Searching Cutting Site for a Specific Sequence Sequence Alignment Search for Conserved Domains ORF Finding

4. Accession Number Gene Sequence Gene Sequence Searching

5. http://www.ncbi.nlm.nih.gov/UniGene/ AA046701 AA069414 AA070289 AA446013 AA425102

8. GGGGGGGGGAAGCTGAGCGCTGAGACCAAGGGCTAAAGCTGGGAGACTGAAAAAATGCAG ACCGCCGGGGCATTATTCATTTCTCCAGCTCTGATCCGCTGTTGTACCAGGGGTCTAATC AGGCCTGTGTCTGCCTCCTTCTTGAATAGCCCAGTGAATTCATCTAAACAGCCTTCCTAC AGCAACTTCCCACTCCAGGTGGCCAGACGGGAGTTCCAGACCAGTGTTGTCTCCCGGGAC ATTGACACAGCAGCCAAGTTTATTGGTGCTGGGGCAGCCACAGTTGGTGTGGCTGGTTCA GGGGCTGGCATTGGAACCGTGTTTGGCAGCTTGATCATTGGCTATGCCAGGAACCCGTCT CTCAAGCAGCAGCTCTTCTCCTATGCCATTCTTGGCTTTGCCCTGTCTGAGGCCATGGGG CTTTTCTGTTTGATGGTCGCCTTCCTCATCCTCTTCGCCATGTGAGGCTCCATGGGGGGT CACCGGCCTGTTGCTACTGCAACTCCACACCATTCTTGGTGCTGGGGTGTGTTAAGCTTT ACCATTAAACACAACGTTTCTCTAAAAAAAAAAAAAAAAAAAAC

9. Gene Sequence Searching Cutting Site for a Specific Sequence Sequence Alignment Search for Conserved Domains ORF Finding

10. Cutting Site for a Specific Sequence Sequence Cut by Restriction Enzymes 1.RestrictionMapper 2.REBASE 3.NEBcutter 4.DARWIN

11. RestrictionMapper http://www.restrictionmapper.org

14. REBASE Rebase.neb.com/ rebase.html

19. DARWIN http://darwin.bio.geneseo.edu/~yin/ WebGene/RE.html

20. Gene Sequence Searching Cutting Site for a Specific Sequence Sequence Alignment Search for Conserved Domains ORF Finding Primary Structure Analysis: Amino acid composition, pI and MW Protein Sequence Searching Proteomics Tools

21. Input Query Amino Acid Sequence DNA Sequence Blastptblastn blastn blastxtblastx Compares Against protein Sequence Database Compares Against Translated Nucleotide Sequence Database Compares Against Nucleotide Sequence Database Compares Against protein Sequence Database Compares Against Translated Nucleotide Sequence Database Sequence Alignment

22. http://www.ncbi.nlm.nih.gov/ BLAST

25. Copy Sequence

29. Pairwise BLAST

32. Gene Sequence Searching Cutting Site for a Specific Sequence Sequence Alignment Search for Conserved Domains ORF Finding

33. Search for Conserved Domains

36. Gene Sequence Searching Cutting Site for a Specific Sequence Sequence Alignment Search for Conserved Domains ORF Finding

37. ORF Finder (Open Reading Frame Finder) http://www.ncbi.nlm.nih.gov/gorf/

40. Useful Bio-Websites euGene http://iubio.bio.indiana.edu:8089/ Proteome Analysis @EBI http://www.ebi.ac.uk/proteome/ GeneCards http://genecards.ym.edu.tw/