1. DNA Extraction PCR Gel Electrophoresis Not Like the Other Miscellaneous $10 $20 $30 $40 $50 Fish DNA Barcoding Game! A Jeopardy-Style Quiz Game Jeopardy is a trademark of Jeopardy Productions, Inc.

2. Answer An organelle other than the nucleus that contains DNA.

3. Question What is a mitochondrion?

4. Answer All of the chromosomal DNA found in a cell.

5. Question What is genomic DNA?

6. Answer The process of rupturing a cell to release its contents.

7. Question What is lysis?

8. Answer A suspension that traps impurities from fish tissue and prevents contamination of DNA.

9. Question What is matrix?

10. Answer The liquid portion above the pellet formed after centrifugation.

11. Question What is the supernatant?

12. Answer Of the four PCR reactions in your table setup today, this is the sample that doesn’t have any DNA in it.

13. Question What is the negative control?

14. DAILY How much do you wish to wager? DOUBLE

15. Daily Double Answer An enzyme used in PCR that is able to function at very high temperatures.

16. Daily Double Question What is Taq polymerase?

17. Answer These target the portion of DNA to be amplified.

18. Question What are primers?

19. Answer A step in PCR in which the temperature is lowered to 50–65ºC, allowing the primers to bind to the DNA.

20. Question What is annealing?

21. Answer The complementary strand of the ACTGGTTA template strand that is being copied during extension.

22. Question What is TGACCAAT?

23. Answer The name of the gene that is being amplified by PCR in the Fish Barcoding Kit.

24. Question What is COI?

25. Answer This acts as a sieve to separate DNA based on its size when an electrical field is applied.

26. Question What is an agarose gel?

27. Answer The size of the amplified portion of the COI gene.

28. Question What is 650 bp?

29. Answer It weighs down the DNA sample so that it stays in the well after loading.

30. Question What is loading dye?

31. Answer It’s used to determine the size of the DNA (in base pairs) in an agarose gel.

32. Question What is a DNA ladder or molecular weight ruler?

33. Answer Where negatively charged DNA migrates to during gel electrophoresis.

34. Question What is the positive or anode side of a gel electrophoresis chamber?

35. Answer P1000 P200 P50 P20

36. Question Which of these is not an actual pipet?

37. Answer DNA extraction RNA extraction PCR Gel electrophoresis

38. Question What is RNA extraction? (not part of today’s experiment)

39. Answer Cod Trout Catfish Mussel

40. Question What is mussel? (not likely to work with fish barcoding kit)

41. Answer Denaturation Annealing Extension Sequencing

42. Question What is sequencing? (not a step in PCR)

43. Answer Thermal cycler Cathode Anode Electrical current

44. Question What is a thermal cycler? (not used in gel electrophoresis)

45. Answer The identification of an organism at the species level using a several hundred base pair sequence of DNA.

46. Question What is barcoding?

47. Answer The order of bases that make up a DNA strand.

48. Question What is a sequence?

49. Answer The database used to identify a mystery fish at the species level.

50. Question What is BOLD?

51. Answer A group of organisms capable of interbreeding and producing fertile offspring.

52. Question What is a species?

53. Answer A place where specimens are stored to support future scientific investigations.

54. Question What is a biorepository?