1. Gene Expression: From Gene to Protein14
Gene Expression: From Gene to Protein
Questions prepared by
Brad Stith, University of Colorado Denver
Louise Paquin, McDaniel College

2. The final product of an expressed gene can be which of the following
The final product of an expressed gene can be which of the following? Also note the function of each.
mRNA
tRNA
rRNA
polypeptide
Answer: D

3. The final product of an expressed gene can be which of the following
The final product of an expressed gene can be which of the following? Also note the function of each.
mRNA
tRNA
rRNA
polypeptide 3

4. Of the following, which pertain to replication, transcription, modification, or translation? Each can be used once or more than once.
origin
anticodon
stop codon
promoter
peptide
terminator sequence
P site
tRNA
GTP
spliceosome
amino acid synthetase 4

5. A. What is the sequence of the nontemplate strand?
The template strand of a given gene includes the sequence 3-G C C A C G T A T C A G-5
A. What is the sequence of the nontemplate strand?
B. What is the mRNA sequence made?
For each one, be sure to indicate 5 and 3 ends.
Answer:
Nontemplate: 5-C G G T G C A T A G T-3
mRNA: 5-C G G U G C A U G U-3 5

6. Which of the following is the best example of gene expression?
A frog adapts to variation in its environmental temperature.
Mouse fur color results from pigment formed by gene-encoded enzymes.
DNA is replicated during the S phase of the cell cycle.
The percent of A versus a alleles in a population is altered by natural selection.
Mutation alters the sequence of a region of DNA.
Answer: B
Gene expression is the first concept of Chapter 14 (albino deer example); also see Concept 14.1. 6

7. Which of the following is the best example of gene expression?
A frog adapts to variation in its environmental temperature.
Mouse fur color results from pigment formed by gene-encoded enzymes.
DNA is replicated during the S phase of the cell cycle.
The percent of A versus a alleles in a population is altered by natural selection.
Mutation alters the sequence of a region of DNA. 7

8. Give some examples that demonstrate the ability of genes from one species to be expressed in a different species. This is possible because of which property of the genetic code?
Answer: Near universality
Figure 14.7 8

9. Describe what is occurring at each of the stages of transcription (1, 2, and 3 in the figure). How does each stage move the process forward?
Answer:
Figure 14.8 9

10. What components form the transcription complex at a eukaryotic promoter?
Answer: TATA box, start point, template, transcription factors, RNA polymerase
Figure 14.9 10

11. How and where are modifications made to eukaryotic mRNA before it goes to the cytosol?
Answer:
Concept 14.3 11

12. What is represented at each of the lettered sites, and what occurs at each of these sites during translation?
Answer:
Figures and 14.17 12

13. On this model of a tRNA molecule, identify each of the following:
5 and 3 ends
hydrogen bonds
amino acid attachment site
anticodon loop
Answer:
Figure 14.15 13

14. Aminoacyl-tRNA synthetase is an enzyme whose function is to ________.
To do so, it binds a molecule of amino acid, and two other molecules: ________ and ________.
After dephosphorylation, which two molecules are joined by a covalent bond?
For the appropriate tRNA to be joined to the amino acid, what must occur?
Answer:
Figure 14.16 14