Presentation on theme: "LATE STAGE LYME DISEASE"— Presentation transcript:
1 LATE STAGE LYME DISEASE Kenny L. De Meirleir, internal medicineEmeritus Gewoon Hoogleraar V.U.B.Clinical Professor of Pathology, University of Nevada, Reno, U.S.A.Medical Director Himmunitas VZW (Brussels) & WPI, University of Nevada, Reno, Nevada, U.S.A.
5 DIAGNOSTIC TESTSBorrelia burgdorferi antibody tests (Elisa, C6 peptide, Western Blot→ mostly negative in LSLDBorrelia PCR tests:Low sensitivity in bloodHigh specificity when combined and confirmed by sequencing (U.L.)Borrelia burgdorferi LTT*:Indirect testValuable in LSLDLTT accepted by FDA in 2011Borrelia culture→ not widely used; still investigationalMicroscopic detection of Borrelia spirochetes:SemenVaginal swap
6 Early treatment (< 2 months): no chronic disease Late treatment: outcome uncertain – many develop “chronic diseases”: ME/CFS, ALS, MS, ...Chronic microglial activation in LSLD – low grade neuroinflammationMarkers blood:sCD14 (LPS)PGE2IL-8Number of CD57+ lymphocytes in the bloodOther pro-inflammatory cytokines and chemokinesNo vaccine available
7 Bartonella – gramnegative bacteria Bartonella henselae – cat scratch diseaseBartonella quintana – trench fever31 other species (8 subspecies are known to infect humans)Only commercial antibody tests for Bartonella henselaePCR with sequencing (only in Belgium)Culture of Bartonella – not approved by FDA yet for clinical useInfects endothelial cells in humansInfect red blood cells – 3-4 Bartonella / millions red blood cellsCats = reservoir: Bartonella / million red blood cells
8 Symptoms Bartonella positive vs. Bartonella negative Pearson Chi-Squareweight change0,005anxiety - which may include panic attacks0,007emotional labiality (mood swings)frequent, unusual nightmares0,002difficulty moving your tongue to speak0,024alcohol intolerance0,015Rash of Herpes Simplex of Shinglesimpotence
13 so all three increased medians for CFS group. Non-parametric Mann – Whitney test performed for two independent groups :Conclusion : for all three variables : MW is significant with p-value < ,so all three increased medians for CFS group.