1. Reproduction of Surubim, Pacu and Arowana ACRSP Project 2007-2008 1 School of Environment and Natural Resources, The Ohio State University, Columbus, Ohio, 43210, USA. 2 Nicolaus Copernicus University, Faculty of Biology and Earth Sciences, 87- 100 Torun, Poland 3 Universidad Nacional Agraria de la Selva, Tingo Maria, Peru 4 Sao Paulo State University, Aquaculture Center, Jaboticabal, Brazil 5 University of Tabasco, Villahermosa, Mexico Konrad Dabrowski 1, Marta Jaroszewska 1,2, Manuel Sandoval 3, Yongfang Zhang 1, Bong-Joo Lee 1, Kyle Ware 1, Rodrigo Takata 4 Maria Celia Portella 4 and Wilfrido Contreras 5 Konrad Dabrowski 1, Marta Jaroszewska 1,2, Manuel Sandoval 3, Yongfang Zhang 1, Bong-Joo Lee 1, Kyle Ware 1, Rodrigo Takata 4 Maria Celia Portella 4 and Wilfrido Contreras 5

2. Successful reproduction and rearing of surubim in North America

3. Embryonic development in Pseudoplatystoma sp. Time after fertilization in hours (h) at 27 o C. Black bars = 1 mm. (A) 1 h, 2-cells; (B) 2 h, 4-cells; (C) 4 h, ½ epiboly; (D) 6 h, blastopore closing; (E) 9 h, first somites; (F) 14 h, at hatching.

4. Surubim catfish (Pseudoplatystoma sp.) February 2007 Columbus, Ohio Yongfang Zhang

5. Growth of pacu (Piaractus mesopotamicus) in Columbus, Ohio. Environmental conditions control included: temperature, pH, and light regime fluctuations. Arrows indicate duration of water temperature cooling and short day light.

6. An immature necropsied pacu scanned and assessed via cross sections. An Aloka (Aloka Co., LTD) SSD-500V ultrasound with a Medi-capture USB100 video capture system with an attached camera, and 3.5 MHz convex array probe. AB = Air bladder; Bc = body cavity wall; F = fin; Fo = food; GB = gall bladder; In = intestine; Ki = kidney; Li = liver; M = muscle; R = ribs; St = stomach; VF = visceral fat. Bong-Joo Lee

7. Work in progress on reproduction of silver arowana (Osteoglossum bicirrhosum) Juveniles 4 weeks after yolk sac absorption, February 24, 2007. Adult, 16 months old, April 2008

8. Extension workshops on reproduction and rearing of tropical fish in Manaus and Balbina, Brasil, May 2007 in Manaus and Balbina, Brasil, May 2007 Manaus Balbina

9. Extention workshops on reproduction and rearing of tropical fish in Tingo Maria and Lima, Peru, July 2007 in Tingo Maria and Lima, Peru, July 2007 Paiche (Arapaima gigas)

10. Research publications Arslan, M., K. Dabrowski, M.C. Portella and J. Rinchard. 2008. The role of dietary lipids and fatty acids in growth and body fatty acid composition of surubim (Pseudoplatystoma fasciatum). J. World Aquacult.Soc. 39: 51-61. Dabrowski, K., M. Arslan, J. Rinchard, M.E. Palacios. 2008. Growth, maturation, induced spawning, and production of the first generation of South American catfish (Pseudoplatystoma sp.) in the North America. J. World Aquacult. Soc. 39: 174-183. Portella, M.C., Dabrowski, K. 2008: Diets, physiology, biochemistry and digestive tract development of freshwater fish larvae. Science Publishers, Inc. (in press). Jaroszewska, M. and Dabrowki, K. 2008. Semionotiformes and Amiiformes. In: (Y.W. Kunz), Development of Non-teleost Fish, Science Publishers, Inc. (in press). Tratner, S., Pickova, J., Park, K.H., Rinchard, J. Dabrowski, K. 2007. Effects of alfa-lipoic and ascorbic acid on the muscle and brain fatty acids and antioxidant profile of the South American pacu Piaractus mesopotamicus. Aquaculture 273: 158-164. Dabrowski, K., Zhang, Y., Arslan, M., Terjesen, B.F. 2007. Indispensable amino acids deprivation does no cause rapid amino acid depletion in fish body and this principle has a potential to be used as the new strategy in nutrition. Revista Colombiana de Ciencias Pecurias 20: 508-511.