1. 7 Feb 05 Part II1 PHARMACOLOGY 545 Cardiovascular Pharmacology Cardiac SL ion transport systems Part II 7 February 2005

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4. 4 Outline Physiological functions of cardiac SL Excitation-contraction coupling Regulation of pH i Metabolism Regulatory receptors Structure of cardiac sarcolemma Excitation-contraction coupling Regulation of pH i

5. 7 Feb 05 Part II5 Na + -Ca 2+ exchanger (NCX)

6. 7 Feb 05 Part II6 NCX 3Na + Ca 2+ E NCX = 3E Na -2E Ca

7. 7 Feb 05 Part II7 E-C coupling in mammalian heart

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12. 7 Feb 05 Part II12 transcribe cDNA into cRNA in vitrotranscribe cDNA into cRNA in vitro inject cRNA into Xenopus oocytesinject cRNA into Xenopus oocytes measuring NCX activity cRN A NCX

13. 7 Feb 05 Part II13 NCX Ca 2+ (8 mM) Na (100 mM) extracellular side cytoplasmic side excised giant patch clamp 1  M

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15. 7 Feb 05 Part II15 Ca 2+ dependence of NCX

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20. 7 Feb 05 Part II20 NCX 3Na + Ca 2+ E NCX = 3E Na -2E Ca

21. 7 Feb 05 Part II21 E-C coupling in mammalian heart

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26. 7 Feb 05 Part II26 Store-operated channels (SOC)

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