1. Introduction to Bio-Engineering HSSP 2402 Leigh Casadaban and Alina Gatowski HSSP 2402 Leigh Casadaban and Alina Gatowski

2. Introductory Stuff Basic info Ice-Breaking learning Basic info Ice-Breaking learning

3. What is Bioengineering?

4. Course 20 says… “ biological engineering, at least as it’s viewed at MIT, is the discipline of using engineering principles and quantitative measurements to be able to both understand and engineer biological systems and molecules.”

5. Engineering + Biology (and Medicine) Anything that involves technology and engineering applied to living systems Not just designing medical devices (Biomedical engineering) Anything that involves technology and engineering applied to living systems Not just designing medical devices (Biomedical engineering)

6. Specialty Areas Biological Imaging and Functional Measurement Biomaterials Biomolecular Engineering Cell and Tissue Engineering Computational Modeling of Biological and Physiological Systems Discovery and Delivery of Molecular Therapeutics Infectious Disease and Immunology Molecular, Cell and Tissue Biomechanics Molecular Epidemiology Nanoscale Engineering of Biological Systems Synthetic Biology Biological Imaging and Functional Measurement Biomaterials Biomolecular Engineering Cell and Tissue Engineering Computational Modeling of Biological and Physiological Systems Discovery and Delivery of Molecular Therapeutics Infectious Disease and Immunology Molecular, Cell and Tissue Biomechanics Molecular Epidemiology Nanoscale Engineering of Biological Systems Synthetic Biology

7. But what does all this mean??

8. To the internet! http://tiny.cc/d6ODL http://parts2.mit.edu/wiki/index.php/ MIT_2006 http://parts2.mit.edu/wiki/index.php/ MIT_2006 http://www.youtube.com/watch?v=n0 UzdYRnMtY http://www.youtube.com/watch?v=n0 UzdYRnMtY http://tiny.cc/d6ODL http://parts2.mit.edu/wiki/index.php/ MIT_2006 http://parts2.mit.edu/wiki/index.php/ MIT_2006 http://www.youtube.com/watch?v=n0 UzdYRnMtY http://www.youtube.com/watch?v=n0 UzdYRnMtY

9. Why these examples are relevant Schmeat Tissue Engineering “Applies the principles of engineering and the life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function” - Prof. Robert Langer Tissue Engineering “Applies the principles of engineering and the life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function” - Prof. Robert Langer

10. Why these examples are relevant iGEM iGEM addresses the question: Can simple biological systems be built from standard, interchangeable parts and operated in living cells? Or is biology simply too complicated to be engineered in this way? Engineer biological devices Bio Bricks Registry of Standard Biological Parts iGEM addresses the question: Can simple biological systems be built from standard, interchangeable parts and operated in living cells? Or is biology simply too complicated to be engineered in this way? Engineer biological devices Bio Bricks Registry of Standard Biological Parts

11. Why these examples are relevant GFP mouse Nobel Prize 2008! In vivo marker Nobel Prize 2008! In vivo marker

12. What kind of stuff do we need to know? The internet again: http://xkcd.com/435/ The internet again: http://xkcd.com/435/

13. Scientific Foundations Biochemistry Organic Chemistry Genetics Cell Biology Thermodynamics Biomechanics Programming + Math, Physics, etc. Biochemistry Organic Chemistry Genetics Cell Biology Thermodynamics Biomechanics Programming + Math, Physics, etc.

14. What We’ll be talking about Biochemistry The study of life in its chemical processes Chemistry, applied to life Biochemistry The study of life in its chemical processes Chemistry, applied to life

15. What do you think this means? or What do you know about biochemistry?

16. why biochemistry is important Do you recognize this?

17. why biochemistry is important How about this?

18. Biochemistry II- Proteins Structure isn’t just important for DNA… How do we go from this… Structure isn’t just important for DNA… How do we go from this…

19. Biochemistry II- Proteins To this… Structure = Function To this… Structure = Function

20. Genetics Why is it important? or What do you know? Why is it important? or What do you know?

21. What is genetics, and why do bioengineers care? At MIT… The topics include: structure and function of genes, chromosomes and genomes, biological variation resulting from recombination, mutation, and selection, population genetics, use of genetic methods to analyze protein function, gene regulation and inherited disease. At MIT… The topics include: structure and function of genes, chromosomes and genomes, biological variation resulting from recombination, mutation, and selection, population genetics, use of genetic methods to analyze protein function, gene regulation and inherited disease.

22. Why is Genetics Important (for bioengineers)? Remember GFP mouse? And the scented E. Coli? Bioengineers use genetic tools like transgenic mice An underlying understanding of the genetic machinery is key Remember GFP mouse? And the scented E. Coli? Bioengineers use genetic tools like transgenic mice An underlying understanding of the genetic machinery is key

23. Thermodynamics What is It?

24. Thermodynamics for Bioengineers http://tiny.cc/LPqzj Wikipedia says… “the study of the conversion of energy into work and heat and its relation to macroscopic variables such as temperature and pressure.” But why is this relevant for us? http://tiny.cc/LPqzj Wikipedia says… “the study of the conversion of energy into work and heat and its relation to macroscopic variables such as temperature and pressure.” But why is this relevant for us?

25. Thermodynamics for Bioengineers Protein folding (Levinthal’s paradox) DNA binding- cooperativity Protein folding (Levinthal’s paradox) DNA binding- cooperativity

26. Programming in Python What do you think programming actually is?? What do you think programming actually is??

27. Programming in Python Solving problems with the help of a computers How to think analytically Learn a new language! Solving problems with the help of a computers How to think analytically Learn a new language!

28. Other Topics Biomechanics and Fluid Dynamics Electromagnetic Forces Biomechanics and Fluid Dynamics Electromagnetic Forces