1. Institutional Biosafety Committee and Biological Use Authorization

2. Biological Use Authorization (BUA) Application BSL 1, ABSL 1 and NIH-Exempt BSL 1, ABSL 1 and NIH-Exempt  Reviewed and approved by BSO BSL 2 or 3, ABSL 2 or 3 and NIH- Non-exempt BSL 2 or 3, ABSL 2 or 3 and NIH- Non-exempt  Reviewed and approved by IBC Currently, no facilities at SDSU meet the minimum criteria for BSL 4; thus this type of work is prohibited.

3. Submission of New BUA New infectious agents New infectious agents New cell lines New cell lines New vector system New vector system Enhanced replication or infectivity Enhanced replication or infectivity Expression of toxic products Expression of toxic products Partial genomes increased to more than two-thirds of whole genome Partial genomes increased to more than two-thirds of whole genome New or altered procedures that pose increased risk (e.g., aerosol or other type of exposure) New or altered procedures that pose increased risk (e.g., aerosol or other type of exposure) Work with non-human systems being changed into work with human system Work with non-human systems being changed into work with human system

4. SDSU Biosafety Approval Institutional Biosafety Committee (IBC)  Protocol Review  Biological Use Authorization (BUA) with respect to containment level (biosafety level)  Training  Inspection

5. Principal Investigator Develops specific protocols to ensure the safe use of biohazardous materials. Develops specific protocols to ensure the safe use of biohazardous materials. Submits a BUA application and obtains approval from IBC prior to commencement of work. Submits a BUA application and obtains approval from IBC prior to commencement of work. Complies with specific biosafety protocols, practices and procedures described in the biohazard control manual. Complies with specific biosafety protocols, practices and procedures described in the biohazard control manual. Ensures that all laboratory staff are appropriately trained on biosafety. Ensures that all laboratory staff are appropriately trained on biosafety. Reports any significant problems, violations of the policies, practices and procedures, or any significant research-related accidents and/or laboratory acquired infection to the BSO immediately. Reports any significant problems, violations of the policies, practices and procedures, or any significant research-related accidents and/or laboratory acquired infection to the BSO immediately.

6. Laboratory Staff Comply with the specific biosafety protocols, practices and procedures described in the biohazard control manual. Comply with the specific biosafety protocols, practices and procedures described in the biohazard control manual. Report to the PI or the lab manager all problems, spills or violations in procedure immediately. Report to the PI or the lab manager all problems, spills or violations in procedure immediately.