1. Group 1 Biology - Chemistry Interface course make up: introductory course emphasizes integration of chemical & biological concepts life science majors ~100 students coreq./prereq. General Chemistry this unit is 2-3 lectures long students spend 4-6 hours outside work

2. Background previous lectures covered: chemical bonding protein structure/function previous lectures covered: chemical bonding protein structure/function

3. Enzyme Unit Overall Learning Goal: Understand what characterizes an enzyme and provide relevant biological examples Overall Learning Goal: Understand what characterizes an enzyme and provide relevant biological examples

4. Unit outcomes Students will be able to: construct a graph that demonstrates the effect of an enzyme on activation energy compare mechanisms of substrate binding compare 2 isozymes that have different affinities (=define kinetic terms, analyze and interpret kinetic data) describe ways that enzyme activity can be regulated

6. Sam, Chris, Ping and Kiran were eager to test the function of an enzyme. They purified an enzyme from living cells and added it to a test tube containing the proper substrate in a buffer. Much to their disappointment, the enzyme failed to function. Brainstorm with your neighbors some possible reasons why the enzyme that they purified failed to function.

8. Learning GoalLearning Outcome to recognize that metal ions often play a critical role in enzyme structure/function generate hypotheses related to enzyme activity during purification analyze how a specific amino acid binds to a metal ion in an enzyme list common metal ions found in enzymes and identify their sources Metal Ion-Protein Interactions

10. How might a metal ion affect enzyme function? - - apo Enzyme (no metal bound) Mg 2 + holo Enzyme (has bound metal) Mg 2 + - - N-terminus C-terminus

11. Mg 2 + - - A) 1 > 2 > 3 B) 2 > 1 > 3 C) 3 > 2 > 1 D) 1 = 2 = 3 E) 2 > 3 > 1 1 - 2 3 Rank from highest to lowest the ability of each of these amino acids to replace the circled amino acid in binding to the magnesium ion:

12. What other metals are important for enzyme structure and/or function? From Nelson and Cox “Lehninger:Principles of Biochemistry”, 5 th edition

13. Why are metalloproteins important to you? http://img.ehowcdn.com/article-page-main/ehow/images/a05/i1/3v/blood-pressure-cuff-bladder_-800x800.jpg

14. Going further... Before the next class, address the following questions on an index card: What metal is the cofactor for Angiotensin Converting Enzyme (ACE)? How could diet affect the function of this enzyme and what could be some health effects?