1. Microbiology Unknown Lab BIO 732

2. TSI agar Triple Sugar Iron Agar (a) Red/red (b) control (c) Red/yellow (d) Yellow/yellow (e) Red/yellow with H 2 S A B C D E

3. Penol Red broths ABCDEABCDE A (acid with gas)…..B (acid)……C (uninoculated control)……D (no recation)… …E (alkaline reaction)

4. O-F tests Control E. coli Pseudomonas Alcaligenes Fermenter Oxidizer Nonsaccharolytic

5. Motility Test

6. Decarboxylation Positive Negative uninoculated

7. Gelatin Hydrolysis

8. Simmons Citrate A B C A: Positive…Enterobacter B: Negative…E. coli C: Control-uninoculated

9. Phenylalanine Deaminase Test neg pos

10. Urea agar pos uninoculated neg

11. Methyl Red Test A B A: Positive (E. coli)B: Negative (Enterobacter aerogenes)

12. Voges Proskauer Test A B A: Negative (E. coli)B: Positive (Enterobacter aerogenes)

13. Indole Test Negative Positive - +

14. Catalase Test + - Positive Negative

15. GRAM STAIN

16. Urea Hydrolysis Positive uninoculated Negative